Enzymatic peracid production using a cosolvent

ABSTRACT

Disclosed herein are two-component enzymatic peracid generation systems and methods of using such systems wherein the first component comprises a formulation of at least one enzyme catalyst having perhydrolysis activity, a carboxylic acid ester substrate, and a cosolvent and wherein the second component comprises a source of peroxygen in water. The two components are combined to produce an aqueous peracid formulation useful as, e.g., a disinfecting or bleaching agent. Specifically, organic cosolvents are used to control the viscosity of a substrate-containing component and to enhance the solubility of the substrate in an aqueous reaction formulation without causing substantial loss of perhydrolytic activity of the enzyme catalyst.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims the benefit of U.S. Provisional Application Nos. 61/102,505; 61/102,512; 61/102,514; 61/102,520; 61/102,531; and 61/102,539; each filed Oct. 3, 2008, each of which incorporated by reference herein in their entireties.

FIELD OF THE INVENTION

The following relates to the field of enzymatic peracid synthesis and in situ enzyme catalysis using multicomponent systems. Specifically, processes are provided to produce and efficaciously deliver peroxycarboxylic acids using the perhydrolysis activity of enzymes identified structurally as belonging to the CE-7 family of carbohydrate esterases, including cephalosporin acetyl hydrolases (CAHs; E.C. 3.1.1.41) and acetyl xylan esterases (AXEs; E.C. 3.1.1.72) using multi-component systems (i.e., in the present context, systems that involve the production of peroxycarboxylic acid using at least two reaction components that are separately stored prior to a desired time of reaction). At least one peroxycarboxylic acid is produced at sufficient concentrations as to be efficacious for the disinfection or sanitization of surfaces, medical instrument sterilization, food processing equipment sterilization, and suitable for use in laundry care applications such as disinfecting, bleaching, destaining, deodorizing, and sanitizing.

BACKGROUND OF THE INVENTION

Peracid compositions have been reported to be effective antimicrobial agents. Methods to clean, disinfect, and/or sanitize hard surfaces, meat products, living plant tissues, and medical devices against undesirable microbial growth have been described (e.g., U.S. Pat. No. 6,545,047; U.S. Pat. No. 6,183,807; U.S. Pat. No. 6,518,307; U.S. Pat. No. 5,683,724; and U.S. Patent Application Publication No, 2003/0026846). Peracids have also been reported to be useful in preparing bleaching compositions for laundry detergent applications (U.S. Pat. No. 3,974,082; U.S. Pat. No. 5,296,161; and U.S. Pat. No. 5,364,554).

Peroxycarboxylic acids can be prepared by the chemical reaction of a carboxylic acid alkyl ester and a peroxide reagent, such as hydrogen peroxide (see Organic Peroxides, Daniel Swern, ed., Vol. 1, pp 313-516; Wiley Interscience, New York, 1971), However, under slightly basic to acidic pH (from about 8 to about 4), the reaction often does not proceed rapidly enough to produce a peroxycarboxylic acid concentration that is suitable for many commercial disinfecting and/or bleaching applications.

One way to overcome the disadvantages of chemical peroxycarboxylic acid production is to use an enzyme catalyst having perhydrolysis activity. U.S. patent application Ser. No. 11/638,635 and U.S. Patent Application Publication Nos. 2008/0176783; 2008/0176299; and 2009/0005590 to DiCosimo et al. disclose enzymes structurally classified as members of the CE-7 family of carbohydrate esterases (e.g., cephalosporin C deacetylases [CAHs] and acetyl xylan esterases [AXEs]) that are characterized by significant perhydrolysis activity for converting carboxylic acid esters (in the presence of a suitable source of peroxygen, such as hydrogen peroxide) into peroxycarboxylic acids at concentrations sufficient for use as a disinfectant and/or a bleaching agent. Some members of the CE-7 family of carbohydrate esterases have been demonstrated to have perhydrolytic activity sufficient to produce 4000-5000 ppm peracetic acid from acetyl esters of alcohols, diols, and glycerols in 1 minute and up to 9000 ppm between 5 minutes and 30 minutes once the reaction components were mixed (DiCosimo of al., U.S. 2009/0005590). The enzymatic peracid generation system described by DiCosimo et al. in each of the cited patent application publications may be based on the use of multiple reaction components that remain separated until the peracid solution is needed.

It has been observed that, when using a multi-component system comprising a first enzyme catalyst/substrate component and a second component comprising an aqueous source of peroxygen, the use of one or more substrates that are insoluble or partially insoluble in water after mixing of the two components can result in at least three conditions that interfere with the ability to efficaciously produce and deliver a peroxycarboxylic acid product: first, the viscosity of the enzyme catalyst/substrate constituent can be too high to permit efficient mixing with a second constituent comprising a source of peroxygen, which decreases the rate of production of peroxycarboxylic acid; second, the viscosity of the enzyme catalyst/substrate constituent can be too high to permit certain modes of delivery of a product comprising a mixture of the enzyme catalyst/substrate constituent and the source of peroxygen, such as spraying; third, the dissolution rate of the substrate in the enzyme/substrate component after mixing with a second component comprising a source of peroxygen in aqueous solution is too low to permit a satisfactory rate of production of peroxycarboxylic acid. These problems also become evident in situations where use of a particular ratio of a component comprising an aqueous source of peroxygen to a component comprising an enzyme catalyst/substrate constituent is desired. As such, commercial uses of multi-component systems that involve the storage of the enzyme catalyst having perhydrolysis activity and substrate separately from a source of peroxygen until a desired time of reaction have remained impracticable for some applications.

The problem to be solved is to provide a method to enzymatically produce peracids when using a multi-component generation system characterized by at least one first component comprising a formulation of a carboxylic acid ester substrate and an enzyme catalyst comprising a CE-7 carbohydrate esterase having perhydrolysis activity, wherein the carboxylic acid ester substrate is (1) partially or substantially insoluble in an aqueous matrix, and/or (2) slow to dissolve into an aqueous reaction matrix and/or (3) has a viscosity that does not facilitate easy mixing for some commercial applications (e.g., use of a two compartment spray bottle designed to mix two liquid components having different viscosities and/or solubilities), and additionally characterized by at least one second component comprising an aqueous solution comprising a source of peroxygen (e.g., an aqueous formulation of hydrogen peroxide).

SUMMARY OF THE INVENTION

The problem has been solved by providing a method and a system to enzymatically produce a peracid that incorporates the use of at least one cosolvent in the first component of a two component system, wherein said first component is a substantially non-aqueous formulation of a carboxylic acid ester and at least one enzyme catalyst comprising a CE-7 carbohydrate esterase having perhydrolysis activity, wherein the addition of the at least one cosolvent improves the viscosity of the first component for delivery and mixing in a two component system, allows for the desired adjustment of the volume of the first component so as to enable mixing with the second component with a desired ratio of the two components, and improves the solubility and/or dissolution rate of the first component when combined with an aqueous second component (i.e., an aqueous formulation providing a source of peroxygen) to form an aqueous peracid formulation.

It has been discovered that the inclusion of a cosolvent comprising an organic solvent having a log P of less than about 2, wherein log P is defined as the logarithm of the partition coefficient of a substance between octanol and water, expressed as log P=log [[solute]_(octanol)/[solute]_(water)], resolves the aforementioned conditions that otherwise interfere with the ability to efficaciously produce and deliver a peroxycarboxylic acid product in a form that can be delivered by means that are conventional to or otherwise suitable in consumer, industrial, and medical contexts. The cosolvent is preferably inert and nonreactive in the formulation and miscible with the carboxylic acid ester substrate, wherein the cosolvent is not a substrate for said enzyme catalyst. The cosolvent is preferably an organic alcohol lacking an enzymatically perhydrolyzable ester group. As used herein, an “alcohol” is molecule comprising at least one hydroxyl moiety. The cosolvent also is preferably soluble at its final concentration following mixing or contacting the components of the multi-component system. However, it has been reported that biocatalysis in organic solvents having the having a log P of less than about 2 often adversely affects enzyme activity (for example, by inactivating the enzyme constituent (see, e.g., C. Laane at, Biotechnol. Bioeng. 30:81-87 (1987) and Cowan, D. A. and Plant, A., Biocatalysis in Organic Phase Systems., Ch 7 in Biocatalysis at Extreme Temperatures, Kelly, R. W. W. and Adams, M., eds., Amer. Chem. Soc. Symposium Series, Oxford University Press, New York, N.Y., pp 86-107 (1992)). As such, the beneficial results provided by the inclusion of a cosolvent comprising an alcohol represent an unexpectedly positive outcome. These and other benefits of the present methods and systems are discussed more fully infra.

In one embodiment, a method for producing a peroxycarboxylic acid is provided comprising

(a) providing a first component comprising:

-   -   (i) a carboxylic acid ester substrate;     -   (ii) an enzyme catalyst having perhydrolysis activity, wherein         said enzyme catalyst comprises an enzyme having a carbohydrate         esterase family 7 (CE-7) signature motif that aligns with SEQ ID         NO: 2 using CLUSTALW, said signature motif comprising;         -   (1) an RGQ motif at amino acid positions aligning with             118-120 of SEQ ID NO:2;         -   (2) a GXSQG motif at amino acid positions aligning with             179-183 of SEQ ID NO:2; and         -   (3) an HE motif at amino acid positions aligning with             298-299 of SEQ ID NO:2;     -   said enzyme comprising at least 30% amino acid identity to SEQ         ID NO: 2; and     -   (iii) at least one cosolvent comprising an organic solvent         having a log P of less than about 2, wherein log P is defined as         the logarithm of the partition coefficient of a substance         between octanol and water, expressed as P         [solute]_(octanol)/[solute]_(water) and wherein the at least one         cosolvent is not a substrate for said enzyme catalyst;

wherein said first component is a substantially non-aqueous mixture of (i)-(iii);

(b) providing a second component comprising a source of peroxygen in water; and (c) combining said first component and said second component to form an aqueous reaction mixture,

wherein said cosolvent solubilizes the carboxylic acid ester substrate in the aqueous reaction mixture without substantial loss of perhydrolytic activity of the enzyme catalyst and whereby peroxycarboxylic acid is produced.

In another aspect, a method for disinfecting a surface comprising performing the method above, further comprising the step of applying said aqueous reaction mixture comprising peroxycarboxylic acid to a surface for disinfection or bleaching.

In another aspect, a method for treating an article of clothing is provided comprising performing the method above, further comprising the step of applying said aqueous reaction mixture comprising peroxycarboxylic acid to the article of clothing for bleaching, stain removal, odor reduction, sanitization, disinfection, or a combination thereof.

Another aspect of the invention is a multi-component system for producing a peroxycarboxylic acid comprising

(a) providing a first component comprising:

-   -   (i) a carboxylic acid ester substrate;     -   (ii) an enzyme catalyst having perhydrolysis activity, wherein         said enzyme catalyst comprises an enzyme having a CE-7 signature         motif that aligns with SEQ ID NO: 2 using CLUSTALW, said         signature motif comprising;         -   (1) an RGQ motif at amino acid positions aligning with             118-120 of SEQ ID NO: 2;         -   (2) a GXSQG motif at amino acid positions aligning with             179-183 of SEQ ID NO: 2; and         -   (3) an HE motif at amino acid positions aligning with             298-299 of SEQ ID NO: 2;     -   said enzyme comprising at least 30% amino acid identity to SEQ         ID NO: 2; and     -   (iii) at least one cosolvent comprising an organic solvent         having a log P of less than about 2, wherein log P is defined as         the logarithm of the partition coefficient of a substance         between octanol and water, expressed as         P=[solute]_(octanol)/[solute]_(water) and wherein the cosolvent         is not a substrate for said enzyme catalyst;

wherein said first component is a substantially non-aqueous mixture of (i)-(iii); and

(b) providing a second component comprising a source of peroxygen in water;

wherein said first component and said second component are combined to produce an aqueous reaction mixture comprising a peroxycarboxylic acid and wherein said cosolvent solubilizes the carboxylic acid ester substrate in the aqueous reaction mixture without substantial loss of perhydrolytic activity of the enzyme catalyst.

The design of the multi-component packaging/delivery systems for separating and combining the first and second components described herein will generally depend upon the physical form of the individual reaction components. In one embodiment, any delivery system suitable for liquid-liquid systems may be used. In another embodiment, the present two-component peracid generation system comprises a first container comprising the present first component and a second container comprising the present second component. Typical examples of containers and/or packing system may include individual bottles, individual bottles packaged together in a single kit, dual chamber dispenser bottles (such as squeeze bottles, spray bottles, and the like.), rigid or non-rigid dual chamber dispenser packets, individual packets, and dissolvable or degradable dual chamber dispenser packets, to name a few. (See U.S. Pat. Nos. 4,678,103; 4,585,150; 6,223,942; 5,954,213; 6,758,411; 5,862,949; 5,398,846; 6,995,125; and 6,391,840; U.S. Published Patent Application Nos. 2005/0139608 and 2002/0030063; and PCT Publication Nos. WO00/61713; WO02/22467; and WO2005/035705). In one embodiment, the delivery system is a dual compartment spray bottle.

In further embodiments, the enzyme catalyst comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO:14, SEQ ID NO: 16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO: 26, SEQ ID NO: 54, SEQ ID NO: 60, SEQ ID NO: 64, SEQ ID NO: 68, SEQ ID NO: 72, SEQ ID NO: 73, and SEQ ID NO: 74 or a substantially similar enzyme having perhydrolase activity derived by substituting, deleting or adding one or more amino acids to said amino acid sequence.

In a further embodiment, the substantially similar enzyme having perhydrolase activity is at least 30%, preferably at least 33%, more preferably at least 40%, more preferably at least 50%, even more preferably at least 60%, yet even more preferable at least 70%, yet even more preferably at least 80%, yet even more preferably at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to one or more amino acid sequences selected from the group consisting of SEQ ID NO: 2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO:14, SEQ ID NO: 16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO: 26, SEQ ID NO: 54, SEQ ID NO: 60, SEQ ID NO: 64, SEQ ID NO; 68, SEQ ID NO: 72, SEQ ID NO: 73, and SEQ ID NO: 74.

In one aspect, the carboxylic acid ester substrate used in the present methods and multi-component systems is selected from the group consisting of:

-   -   (a) one or more esters having the structure

[X]_(m)R₅

-   -   wherein         -   X is an ester group of the formula R₆C(O)O;     -   R₆ is a C1 to C7 linear, branched or cyclic hydrocarbyl moiety,         optionally substituted with a hydroxyl group or C1 to C4 alkoxy         group, wherein R₆ optionally comprises one or more ether         linkages where R₆ is C2 to C7;         -   R₅ is a C1 to C6 linear, branched, or cyclic hydrocarbyl             moiety optionally substituted with a hydroxyl group, wherein             each carbon atom in R₅ individually comprises no more than             one hydroxyl group or no more than one ester group, and             wherein R₅ optionally comprises one or more ether linkages;     -   m is 1 to the number of carbon atoms in R₅,     -   said one or more esters having solubility in water of at least 5         ppm at 25° C.;     -   (b) one or more glycerides having the structure

-   -   -   wherein R₁ is a C1 to C7 straight chain or branched chain             alkyl optionally substituted with a hydroxyl or a C1 to C4             alkoxy group and R₃ and R₄ are individually H or R₁C(O);

    -   (c) one or more esters of the formula

-   -   wherein R₁ is a C1 to C7 straight chain or branched chain alkyl         optionally substituted with an hydroxyl or a C1 to C4 alkoxy         group and R₂ is a C1 to C10 straight chain or branched chain         alkyl, alkenyl, alkynyl, aryl, alkylaryl, alkylheteroaryl,         heteroaryl, (CH₂CH₂O)_(n), or (CH₂CH(CH₃)—O)_(n)H and n is 1 to         10;     -   (d) one or more acetylated monosaccharides, acetylated         disaccharides, or acetylated polysaccharides; and     -   (e) any combination of (a) through (d);

In one embodiment, the carboxylic acid ester substrate is selected from the group consisting of; monoacetin; triacetin; monopropionin; dipropionin; tripropionin; monobutyrin; dibutyrin; tributyrin; glucose pentaacetate; xylose tetraacetate; acetylated xylan; acetylated xylan fragments; β-D-ribofuranose-1,2,3,5-tetraacetate; tri-O-acetyl-D-galactal; tri-O-acetyl-glucal; propylene glycol diacetate, ethylene glycol diacetate; monoesters or diesters of 1,2-ethanediol; 1,2-propanediol; 1,3-propanediol; 1,2-butanediol; 1,3-butanediol; 2,3-butanediol; 1,4-butanediol; 1,2-pentanediol; 2,5-pentanediol; 1,6-pentanediol; 1,2-hexanediol; 2,5-hexanediol; 1,6-hexanediol; and mixtures thereof. In another embodiment the substrate is selected from the group consisting of propylene glycol diacetate, ethylene glycol diacetate, and mixtures thereof. In further embodiment, the substrate preferably comprises triacetin.

Unless otherwise specified, disclosure of a particular embodiment applies equally to the present methods for producing peroxycarboxylic acid in a multi-component system, the present methods for disinfecting a surface, the present multi-component formulation compositions, and the present generation systems for producing peroxycarboxylic acid.

In a further aspect, the present method and systems may also be used in laundry care applications to produce a beneficial effect including, but not limited to, bleaching, destaining, deodorizing, and sanitizing.

BRIEF DESCRIPTION OF THE FIGURE

FIG. 1, sheets A-F, show the results of a CLUSTALW alignment (version 1.83) of several enzymes structurally classified as carbohydrate esterase family 7 members including SEQ ID NOs 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 54, 60, 64, 68, 72, 73, and 74. All of the enzymes share the conserved motifs (underlined) that together form the signature motif for CE-7 carbohydrate esterases (see Vincent of al., J. Mol. Biol., 330:593-606 (2003) and U.S. Patent Application Publication No. 2008/0176783 to DiCosimo et al.). An additional motif that may be used to further characterize members of the CE-7 family of enzymes is underlined and bold (i.e., the LXD motif).

BRIEF DESCRIPTION OF THE BIOLOGICAL SEQUENCES

The following sequences comply with 37 C.F.R. §§1.821-1.825 (“Requirements for Patent Applications Containing Nucleotide Sequences and/or Amino Acid Sequence Disclosures—the Sequence Rules”) and are consistent with World Intellectual Property Organization (WIPO) Standard ST.25 (1998) and the sequence listing requirements of the European Patent Convention (EPC) and the Patent Cooperation Treaty (PCT) Rules 5.2 and 49.5(a-bis), and Section 208 and Annex C of the Administrative Instructions. The symbols and format used for nucleotide and amino acid sequence data comply with the rules set forth in 37 C.F.R. §1.822.

SEQ ID NO: 1 is the nucleic acid sequence of the cephalosporin C deacetylase (cah) coding region from Bacillus subtilis ATCC® 31954™.

SEQ ID NO: 2 is the deduced amino acid sequence of the cephalosporin C deacetylase from Bacillus subtilis ATCC® 31954™.

SEQ ID NO: 3 is the nucleic acid sequence of the cephalosporin C deacetylase coding region from B. subtilis subsp. subtilis str. 168.

SEQ ID NO: 4 is the deduced amino acid sequence of the cephalosporin C deacetylase from B. subtilis subsp. subtilis str. 168, and is identical to the deduced amino acid sequence of the cephalosporin C deacetylase from B. subtilis BE1010.

SEQ ID NO: 5 is the nucleic acid sequence of the cephalosporin acetylesterase coding region from B. subtilis ATCC® 6633™.

SEQ ID NO: 6 is the deduced amino acid sequence of the cephalosporin acetylesterase from B. subtilis ATCC® 6633™.

SEQ ID NO: 7 is the nucleic acid sequence of the cephalosporin C deacetylase coding region from B. licheniformis ATCC® 14580™.

SEQ ID NO: 8 is the deduced amino acid sequence of the cephalosporin C deacetylase from B. licheniformis ATCC® 14580™.

SEQ ID NO: 9 is the nucleic acid sequence of the acetyl xylan esterase coding region from B. pumilus PS213.

SEQ ID NO: 10 is the deduced amino acid sequence of the acetyl xylan esterase from B. pumilus PS213.

SEQ ID NO: 11 is the nucleic acid sequence of the acetyl xylan esterase coding region from Clostridium thermocellum ATCC® 27405™.

SEQ ID NO: 12 is the deduced amino acid sequence of the acetyl xylan esterase from Clostridium thermocellum ATCC® 27405™.

SEQ ID NO: 13 is the nucleic acid sequence of the acetyl xylan esterase coding region from Thermotoga neapolitana.

SEQ ID NO: 14 is the deduced amino acid sequence of the acetyl xylan esterase from Thermotoga neapolitana.

SEQ ID NO: 15 is the nucleic acid sequence of the acetyl xylan esterase coding region from Thermotoga maritima MSB8.

SEQ ID NO: 16 is the deduced amino acid sequence of the acetyl xylan esterase from Thermotoga maritima MSB8.

SEQ ID NO: 17 is the nucleic acid sequence of the acetyl xylan esterase coding region from Thermoanaerobacterium sp. JW/SL YS485.

SEQ ID NO: 18 is the deduced amino acid sequence of the acetyl xylan esterase from Thermoanaerobacterium sp. JW/SL YS485.

SEQ ID NO: 19 is the nucleic acid sequence encoding the cephalosporin C deacetylase from Bacillus sp. NRRL B-14911 as reported in GENBANK® Accession number ZP_(—)01168674. However, the reported sequence appears to have a 15 amino acid N-terminal addition that is likely incorrect based on sequence alignments with other cephalosporin C deacetylases and a comparison of the reported length (340 amino acids) versus the observed length of other CAH enzymes (typically 318-325 amino acids in length).

SEQ ID NO: 20 is the deduced amino acid sequence of the cephalosporin C deacetylase from Bacillus sp. NRRL B-14911 without the N-terminal 15 amino acids reported under GENBANK® Accession number ZP_(—)01168674.

SEQ ID NO: 21 is the nucleic acid sequence of the cephalosporin C deacetylase coding region from Bacillus halodurans C-125.

SEQ ID NO: 22 is the deduced amino acid sequence of the cephalosporin C deacetylase from Bacillus halodurans C-125.

SEQ ID NO: 23 is the nucleic acid sequence of the cephalosporin C deacetylase coding region from Bacillus clausii KSM-K16.

SEQ ID NO: 24 is the deduced amino acid sequence of the cephalosporin C deacetylase from Bacillus clausii KSM-K16.

SEQ ID NO: 25 is the nucleic acid sequence of the Bacillus subtilis ATCC 29233™ cephalosporin C deacetylase (cab) gene cloned into pSW190.

SEQ ID NO: 26 is the deduced amino acid sequence of the Bacillus subtilis ATCC® 29233™ cephalosporin C deacetylase (CAH).

SEQ ID NOs: 27 and 28 are primers used to PCR amplify the Thermotoga neapolitana acetyl xylan esterase coding region (GENBANK® U58632) for construction of pSW196.

SEQ ID NO: 29 is the nucleic acid sequence of the codon-optimized version of the Thermotoga neapolitana acetyl xylan esterase gene in plasmid pSW196.

SEQ ID NO: 30 is the nucleic acid sequence of the kanamycin resistance gene (kan).

SEQ ID NO: 31 is the nucleic acid sequence of plasmid pKD13.

SEQ ID NOs: 32 and 33 are primers used to generate a PCR product encoding the kanamycin gene flanked by regions having homology to the katG catalase gene in E. coli MG1655. The product was used to disrupt the endogenous katG gene.

SEQ ID NO: 34 is the nucleic acid sequence of the PCR product encoding the kanamycin resistance gene flanked by regions having homology to the katG catalase gene in E. coli MG1655. The product was used to disrupt the endogenous katG gene.

SEQ ID NO: 35 is the nucleic acid sequence of the katG catalase gene in E. coli MG1655.

SEQ ID NO: 36 is the deduced amino acid sequence of the KatG catalase in E. coli MG1655.

SEQ ID NO: 37 is the nucleic acid sequence of plasmid pKD46.

SEQ ID NOs: 38 and 39 are primers used to confirm the disruption of the katG gene.

SEQ ID NO: 40 is the nucleic acid sequence of plasmid pCP20.

SEQ ID NOs: 41 and 42 are primers used to generate a PCR product encoding the kanamycin gene flanked by regions having homology to the katE catalase gene in E. coli MG1655. The product was; used to disrupt the endogenous katE gene.

SEQ ID NO: 43 is the nucleic acid sequence of the PCR product encoding the kanamycin resistance gene flanked by regions having homology to the katE catalase gene in E. coli MG1655. The product was used to disrupt the endogenous katE gene.

SEQ ID NO: 44 is the nucleic acid sequence of the katE catalase gene in E. coli MG1655.

SEQ ID NO: 45 is the deduced amino acid sequence of the KatE catalase in E. coli MG1655.

SEQ ID NOs: 46 and 47 are primers used to confirm disruption of the katE gene in the single knockout strain E. coli MG1655 ΔkatE, and in the double-knockout strain E. coli MG1655 ΔkatG ΔkatE, herein referred to as E. coli KLP18.

SEQ ID NO: 48 is the nucleic acid sequence of the codon optimized version of the Bacillus pumilus PS213 encoding the amino acid sequence SEQ ID NO: 10.

SEQ ID NO: 49 is the amino acid sequence of the region encompassing amino acids residues 118 through 299 of SEQ ID NO: 2.

SEQ ID NO: 50 is the nucleic acid sequence of the PCR product encoding the codon-optimized version of the Bacillus clausii KSM-K16 cephalosporin-C deacetylase coding sequence.

SEQ ID NO: 51 is the nucleic acid sequence of the codon-optimized Bacillus clausii KSM-K16 cephalosporin-C deacetylase coding sequence.

SEQ ID NO: 52 is the nucleic acid sequence of the PCR product encoding the codon-optimized version of the Thermoanaerobacterium saccharolyticum acetyl xylan esterase coding sequence.

SEQ ID NO: 53 is the nucleic acid sequence of the codon-optimized version of the Thermoanaerobacterium saccharolyticum acetyl xylan esterase coding sequence.

SEQ ID NO: 54 is the deduced amino acid sequence of the acetyl xylan esterase from Thermoanaerobacterium saccharolyticum (GENBANK®Accession No. S41858).

SEQ ID NO: 55 is the nucleic acid sequence of the PCR product encoding the codon-optimized version of the Thermotoga maritima MSB8 acetyl xylan esterase coding sequence.

SEQ ID NO: 56 is the nucleic acid sequence of the codon-optimized version of the Thermotoga maritima MSB8 acetyl xylan esterase coding sequence.

SEQ ID NO: 57 is the nucleic acid sequence of the PCR product encoding the codon-optimized version of the Thermotoga lettingae acetyl xylan esterase coding sequence.

SEQ ID NO: 58 is the nucleic acid sequence of the PCR product encoding the codon-optimized version of the Thermotoga lettingae acetyl xylan esterase coding sequence.

SEQ ID NO: 59 is the nucleic acid sequence of the acetyl xylan esterase coding region from Thermotoga lettingae.

SEQ ID NO: 60 is the deduced amino acid sequence of the acetyl xylan esterase from Thermotoga lettingae.

SEQ ID NO: 61 is the nucleic acid sequence of the PCR product encoding the codon-optimized version of the Thermotoga petrophila acetyl xylan esterase coding sequence.

SEQ ID NO: 62 is the nucleic acid sequence of the PCR product encoding a codon-optimized version of the Thermotoga petrophila acetyl xylan esterase coding sequence.

SEQ ID NO: 63 is the nucleic acid sequence of the acetyl xylan esterase coding region from Thermotoga petrophila.

SEQ ID NO: 64 is the deduced amino acid sequence of an acetyl xylan esterase from Thermotoga petrophila

SEQ ID NO: 65 is the nucleic acid sequence of the PCR product encoding the codon-optimized version of the Thermotoga sp. RQ2 “RQ2(a)” acetyl xylan esterase coding sequence.

SEQ ID NO: 66 is the nucleic acid sequence of the PCR product encoding a codon-optimized version of the Thermotoga sp. RQ2 “RQ2(a)” acetyl xylan esterase coding sequence.

SEQ ID NO: 67 is the nucleic acid sequence of the acetyl xylan esterase coding region from Thermotoga sp. RQ2 identified herein as “RQ2(a)”.

SEQ ID NO: 68 is the deduced amino acid sequence of an acetyl xylan esterase (GENBANK® Accession No. ACB09222) from Thermotoga sp. RQ2 identified herein as “RQ2(a)”.

SEQ ID NO: 69 is the nucleic acid sequence of the PCR product encoding the codon-optimized version of the Thermotoga sp. RQ2 “RQ2(b)” acetyl xylan esterase coding sequence.

SEQ ID NO: 70 is the nucleic acid sequence of the PCR product encoding a codon-optimized version of the Thermotoga sp. RQ2 “RQ2(b)” acetyl xylan esterase coding sequence.

SEQ ID NO: 71 is the nucleic acid sequence of the acetyl xylan esterase coding region from Thermotoga sp. RQ2 identified herein as “RQ2(b)”.

SEQ ID NO: 72 is the deduced amino acid sequence of an acetyl xylan esterase (GENBANK® Accession No. ACB08860) from Thermotoga sp. RQ2 identified herein as “RQ2(b)”.

SEQ ID NO: 73 is the deduced amino acid sequence of a Thermotoga neapolitana acetyl xylan esterase variant from co-owned, co-filed, and copending U.S. patent application Attorney Docket No. CL4392 US NA (incorporated herein by reference in its entirety), where the Xaa residue at position 277 is Ala, Val, Ser, or Thr.

SEQ ID NO: 74 is the deduced amino acid sequence of a Thermotoga maritima MSB8 acetyl xylan esterase variant from co-owned, co-filed, and copending U.S. patent application Attorney Docket No. CL4392 US NA, where the Xaa residue at position 277 is Ala, Val, Ser, or Thr.

DETAILED DESCRIPTION OF THE INVENTION

In certain applications for a multicomponent in situ peracid disinfectant formulation, it may be desirable for the ratio of the second component (comprising an aqueous source of peroxygen) to the first component (comprising the enzyme and the enzyme substrate) to be within a range of from 1:1 to 10:1, where from 10 parts to 1 part (by weight) of the second component is mixed with one part (by weight) of the first component to produce a peracid at a concentration efficacious for disinfection.

The stated problems have been solved by the discovery that, in multicomponent methods and systems in the present context, methods and systems that involve the production of peroxycarboxylic acid using at least two reaction components that are separately stored prior to a desired time of reaction), substrates of perhydrolases can be efficaciously mixed with a source of peroxygen in water and satisfactorily delivered, for example, to a surface, through the inclusion of a cosolvent. As described herein, addition of an organic solvent to a catalyst composition (Component A; herein also referred to as the “first” component) and removal of an equal volume of water from the aqueous source of peroxygen (Component B; herein also referred to as the “second” component), such that the ratio of Component B to Component A is within a range of from 1:1 to 10:1, allows production of a peracid at a concentration efficacious for disinfection. The organic solvent is preferably inert and non-reactive in the formulation. The cosolvent also is preferably completely miscible with the enzyme substrate (e.g., triacetin). The cosolvent also is preferably soluble at its final concentration in the combined Components A and B after mixing. Pursuant to the present invention, the CE-7 carbohydrate esterase family of structurally-related enzymes can be used in multicomponent systems to generate concentrations of peracids with high efficiency for disinfection and/or bleaching applications, and, as described for fully infra, cosolvents comprising organic solvents can surprisingly be used to enhance the solubility of the substrate in an aqueous reaction formulation without substantial loss of perhydrolytic activity of the enzyme catalyst. In a further aspect, the present invention includes methods and multi-component systems for use in laundry care applications wherein an article of clothing or a textile is contacted with peracids at concentrations suitable for bleaching, stain removal, odor reduction, sanitization, disinfection, or a combination thereof.

The present invention may be understood more readily by reference to the following detailed description taken in connection with the accompanying FIGURE, the sequence listing, and examples, which form a part of this disclosure. It is to be understood that this invention is not limited to the specific products, methods, conditions or parameters described and/or shown herein, and that the terminology used herein is for the purpose of describing particular embodiments by way of example only and is not intended to be limiting of the claimed invention.

In this disclosure, a number of terms and abbreviations are used. The following definitions apply unless specifically stated otherwise.

As used herein, the articles “a”, “an”, and “the” preceding an element or component of the invention are intended to be nonrestrictive regarding the number of instances (i.e., occurrences) of the element or component. Therefore “a”, “an” and “the” should be read to include one or at least one, and the singular word form of the element or component also includes the plural unless the number is obviously meant to be singular.

As used herein, the term “comprising” means the presence of the stated features, integers, steps, or components as referred to in the claims, but that it does not preclude the presence or addition of one or more other features, integers, steps, components or groups thereof. The term “comprising” is intended to include embodiments encompassed by the terms “consisting essentially of” and “consisting of”. Similarly, the term “consisting essentially of” is intended to include embodiments encompassed by the term “consisting of”.

As used herein, the term “about” modifying the quantity of an ingredient or reactant employed refers to variation in the numerical quantity that can occur, for example, through typical measuring and liquid handling procedures used for making concentrates or use solutions in the real world; through inadvertent error in these procedures; through differences in the manufacture, source, or purity of the ingredients employed to make the compositions or carry out the methods; and the like. The term “about” also encompasses amounts that differ due to different equilibrium conditions for a composition resulting from a particular initial mixture. Whether or not modified by the term “about”, the claims include equivalents to the quantities.

Where present, all ranges are inclusive and combinable. For example, when a range of “1 to 5” is recited, the recited range should be construed as including ranges “1 to 4”, “1 to 3”, “1-2”, “1-2 & 4-5”, “1-3 & 5”, and the like.

As used herein, the term “peracid” is synonymous with peroxyacid, peroxycarboxylic acid, peroxy acid, percarboxylic acid and peroxoic acid.

As used herein, the term “peracetic acid” is abbreviated as “PAA” and is synonymous with peroxyacetic acid, ethaneperoxoic acid and all other synonyms of CAS Registry Number 79-21-0.

As used herein, the terms “substrate”, “suitable substrate”, and “carboxylic acid ester substrate” interchangeably refer specifically to:

-   -   (a) one or more esters having the structure

[X]_(m)R₅

-   -   wherein     -   X is an ester group of the formula R₆C(O)O;     -   R₆ is a C1 to C7 linear, branched or cyclic hydrocarbyl moiety,         optionally substituted with a hydroxyl group or C1 to C4 alkoxy         group, wherein R₅ optionally comprises one or more ether         linkages where R₆ is C2 to C7;     -   R₅ is a C1 to C6 linear, branched, or cyclic hydrocarbyl moiety         optionally substituted with a hydroxyl group, wherein each         carbon atom in R₅ individually comprises no more than one         hydroxyl group or no more than one ester group, and wherein R₅         optionally comprises one or more ether linkages;     -   m is 1 to the number of carbon atoms in R₅,     -   said one or more esters having solubility in water of at least 5         ppm at 25° C.; or     -   (b) one or more glycerides having the structure

-   -   wherein R₁ is a C1 to C7 straight chain or branched chain alkyl         optionally substituted with an hydroxyl or a C1 to C4 alkoxy         group and R₃ and R₄ are individually H or R₁C(O); or     -   (c) one or more esters of the formula

-   -   wherein R₁ is a C1 to C7 straight chain or branched chain alkyl         optionally substituted with an hydroxyl or a C1 to C4 alkoxy         group and R₂ is a C1 to C10 straight chain or branched chain         alkyl, alkenyl, alkynyl, aryl, alkylaryl, alkylheteroaryl,         heteroaryl, (CH₂CH₂O)_(n), or (CH₂CH(CH₃)—O)_(n)H and n is 1 to         10; or     -   (d) one or more acetylated monosaccharides, acetylated         disaccharides, or acetylated polysaccharides; or     -   (e) any combination of (a) through (d).

Examples of said carboxylic acid ester substrate may include monoacetin; triacetin; monopropionin; dipropionin; tripropionin; monobutyrin; dibutyrin; tributyrin; glucose pentaacetate; xylose tetraacetate; acetylated xylan; acetylated xylan fragments; β-D-ribofuranose-1,2,3,5-tetraacetate; tri-O-acetyl-D-galactal; tri-O-acetyl-glucal; propylene glycol diacetate; ethylene glycol diacetate; monoesters or diesters of 1,2-ethanediol; 1,2-propanediol; 1,3-propanediol; 1,2-butanediol; 1,3-butanediol; 2,3-butanediol; 1,4-butanediol; 1,2-pentanediol; 2,5-pentanediol; 1,6-pentanediol, 1,2-hexanediol; 2,5-hexanediol; 1,6-hexanediol; or any combination thereof.

As used herein, the term “monoacetin” is synonymous with glycerol monoacetate, glycerin monoacetate, and glyceryl monoacetate.

As used herein, the term “diacetin” is synonymous with glycerol diacetate; glycerin diacetate, glyceryl diacetate, and all other synonyms of CAS Registry Number 25395-31-7.

As used herein, the term “triacetin” is synonymous with glycerin triacetate; glycerol triacetate; glyceryl triacetate, 1,2,3-triacetoxypropane, 1,2,3-propanetriol triacetate and all other synonyms of GAS Registry Number 102-76-1.

As used herein, the term “monobutyrin” is synonymous with glycerol monobutyrate, glycerin monobutyrate, and glyceryl monobutyrate.

As used herein, the term “dibutyrin” is synonymous with glycerol dibutyrate and glyceryl dibutyrate.

As used herein, the term “tributyrin” is synonymous with glycerol tributyrate, 1,2,3-tributyrylglycerol, and all other synonyms of GAS Registry Number 60-01-5.

As used herein, the term “monopropionin” is synonymous with glycerol monopropionate, glycerin monopropionate, and glyceryl monopropionate.

As used herein, the term “dipropionin” is synonymous with glycerol dipropionate and glyceryl dipropionate.

As used herein, the term “tripropionin” is synonymous with glyceryl tripropionate, glycerol tripropionate, 1,2,3-tripropionylglycerol, and all other synonyms of CAS Registry Number 13945-7.

As used herein, the term “ethyl acetate” is synonymous with acetic ether, acetoxyethane, ethyl ethanoate, acetic acid ethyl ester, ethanoic acid ethyl ester, ethyl acetic ester and all other synonyms of CAS Registry Number 141-78-6.

As used herein, the term “ethyl lactate” is synonymous with lactic acid ethyl ester and all other synonyms of CAS Registry Number 97-64-3.

As used herein, the terms “acetylated sugar” and “acetylated saccharide” refer to mono-, di- and polysaccharides comprising at least one acetyl group. Examples include, but are not limited to, glucose pentaacetate, xylose tetraacetate, acetylated xylan, acetylated xylan fragments, β-D-ribofuranose-1,2,3,5-tetraacetate, tri-O-acetyl-D-galactal, and tri-O-acetyl-glucal.

As used herein, the terms “hydrocarbyl”, “hydrocarbyl group”, and “hydrocarbyl moiety” is meant a straight chain, branched or cyclic arrangement of carbon atoms connected by single, double, or triple carbon to carbon bonds and/or by ether linkages, and substituted accordingly with hydrogen atoms. Such hydrocarbyl groups may be aliphatic and/or aromatic. Examples of hydrocarbyl groups include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, t-butyl, cyclopropyl, cyclobutyl, pentyl, cyclopentyl, methylcyctopentyl, hexyl, cyclohexyl, benzyl, and phenyl. In a preferred embodiment, the hydrocarbyl moiety is a straight chain, branched or cyclic arrangement of carbon atoms connected by single carbon to carbon bonds and/or by ether linkages, and substituted accordingly with hydrogen atoms.

As used herein, the terms “monoesters” and “diesters” of 1,2-ethanediol, 1,2-propanediol, 1,3-propanediol, 1,2-butanediol, 1,3-butanediol, 2,3-butanediol, 1,4-butanediol, 1,2-pentanedial, 2,5-pentanediol, 1,6-pentanedial, 1,2-hexanediol, 2,5-hexanediol, 1,6-hexanediol, and mixtures thereof refer to said compounds comprising at least one ester group of the formula RC(O)O, wherein R is a C1 to C7 linear hydrocarbyl moiety. In one embodiment, the carboxylic acid ester substrate comprises propylene glycol diacetate (PGDA), ethylene glycol diacetate (EDGA) or mixtures thereof.

As used herein, the term “propylene glycol diacetate” is synonymous with 1,2-diacetoxypropane, propylene diacetate, 1,2-propanediol diacetate, and all other synonyms of CAS Registry Number 623-84-7.

As used herein, the term “ethylene glycol diacetate” is synonymous with 1,2-diacetoxyethane, ethylene diacetate, glycol diacetate, and all other synonyms of CAS Registry Number 111-55-7.

As used herein, the terms “suitable enzymatic reaction mixture”, “components suitable for in situ generation of a peracid”, “suitable reaction components”, “suitable aqueous reaction mixture”, and “reaction mixture” refer to the materials and water in which the reactants and enzyme catalyst come into contact. The components of the suitable aqueous reaction mixture are provided herein and those skilled in the art appreciate the range of component variations suitable for this process. In one embodiment, the suitable enzymatic reaction mixture produces peracid in situ upon combining the reaction components. As such, the reaction components may be provided as a multi-component system wherein the reaction components remains separated until use. The design of systems and means for separating and combining multiple active components are known in the art and generally will depend upon the physical form of the individual reaction components. For example, multiple active fluids (liquid-liquid) systems typically use multichamber dispenser bottles or two-phase systems (e.g., U.S. Patent Application Publication No. 2005/0139608; U.S. Pat. No. 5,398,846; U.S. Pat. No. 5,624,634; U.S. Pat. No. 6,391,840; E.P. Patent 0807156B1; U.S. Patent Application Publication No. 2005/0008526; and PCT Publication No. WO 00/61713) such as found in some bleaching applications wherein the desired bleaching agent is produced upon mixing the reactive fluids. Other forms of multi-component systems used to generate peracid may include, but are not limited to, those designed for one or more solid components or combinations of solid-liquid components, such as powders (e.g., U.S. Pat. No. 5,116,575), multi-layered tablets (e.g., U.S. Pat. No. 6,210,639), water dissolvable packets having multiple compartments (e.g., U.S. Pat. No. 6,995,125) and solid agglomerates that react upon the addition of water (e.g., U.S. Pat. No. 6,319,888).

In multi-component systems, active constituents are initially separated from each other in one or more respective components and then combined to form a reaction formulation. A multi-component system can face such problems as the failure of the active components to satisfactorily combine, the neutralization or reduction of the activity of one or more components, and/or the formation of a reaction formulation that is not compatible with delivery requirements. For example, in a multi-component system including at least one component comprising an enzyme and a substrate for such enzyme that is insoluble or partially insoluble in a second component comprising water, at least three conditions may arise that interfere with the ability to efficaciously produce and deliver a peroxycarboxylic acid product: first, the viscosity of the enzyme/substrate constituent can be too high to permit efficient mixing with a second constituent comprising a source of peroxygen, which decreases the rate of production of peroxycarboxylic acid; second, the viscosity of the enzyme/substrate constituent can be too high to permit certain modes of delivery of a product comprising a mixture of the enzyme/substrate constituent and the source of peroxygen, such as spraying; third, the dissolution rate of the substrate in the enzyme/substrate component after mixing with a second component comprising a source of peroxygen in aqueous solution is too low to permit a satisfactory rate of production of peroxycarboxylic acid. These problems also become evident in situations where use of a particular ratio of a component comprising an aqueous source of peroxygen to a component comprising an enzyme/substrate constituent is desired.

It is well known to those skilled in the art that organic solvents can be deleterious to the activity of enzymes, either when enzymes are suspended directly in organic solvents, or when miscible organic/aqueous single phase solvents are employed. Two literature publications that review the effects of organic solvents on enzyme activity and structure are: (a) C. Laane et al., supra and (b) D. A. Cowan and A. R. Plant, supra. Cowan and Plant note (on page 87) that the art generally recognizes that there is little or no value in using organic solvents having a log P≦2 to stabilize intracellular enzymes in an organic phase system, where log P is defined as the logarithm of the partition coefficient of a substance between octanol and water, expressed as P=[solute]_(octanol)/[solute]_(water). Organic solvents having a log P between 2 and 4 can be used on a case-by-case basis dependent on enzyme stability, and those having a log P>4 are generally useful in organic phase systems.

Cowan and Plant further note (on page 91) that the effect of direct exposure of an enzyme dissolved in a single-phase organic-aqueous solvent depends on solvent concentration, solvent/enzyme surface group interactions, and solvent/enzyme hydration shell interactions. Because a solvent's log P must be sufficiently low so that the solvent is fully miscible with the aqueous phase to produce a single-phase, a single-phase organic-aqueous solvent containing a low log P organic solvent usually has a negative effect on enzyme stability except in low organic solvent concentration applications. Thus, organic solvents having a low log P are traditionally thought to be detrimental to enzyme stability at anything but unworkably low concentrations.

Triacetin is reported to have a log P of 0.25 (Y. M. Gunning, et al., J. Agric. Food Chem. 48:395-399 (2000)), similar to that of ethanol (log P −0.26) and isopropanol (log P 0.15) (Cowan and Plant); therefore the storage of enzyme powder in triacetin would be expected to result in unacceptable loss of enzyme activity, as would the use of additional cosolvents with log P<2 (e.g., cyclohexanone, log P=0.94) (Cowan and Plant); 1,2-propanediol, log P=−1.41 (Gunning, et al.); 1,3-propanediol, log P=−1.3 (S-J, Kuo, et al., J. Am. Oil Chem. Soc. 73:1427-1433 (1996); diethylene glycol butyl ether, log P=0.56 (N. Funasaki, et al., J. Phys. Chem. 68:5786-5790 (1984); triethyleneglycol, log P=−1.75 (L. Braeken, et al., ChemPhysChem 6:1606-1612 (2005)). Applying the above-referenced teachings of Cowan and Plant, it would be expected that the solvents listed above, having low log P values, could not suitably be included in an enzyme-containing first component of a multicomponent system without inactivation of the enzyme (either prior to or after mixing of the first component with a second component comprising a source of peroxygen in water).

However, it has surprisingly been discovered that the inclusion of a cosolvent comprising an organic solvent having a log P of less than about 2 may function to aid in the dissolution of enzyme substrate with poor solubility in water and/or may function as a diluent of Component A to enable mixing of a desired ratio of Components A and B. In other words, the cosolvent may resolve conditions (e.g., unacceptably high viscosity of an enzyme/substrate component, poor mixing of this component with a source of peroxygen in water) that otherwise interfere with the ability to efficaciously produce and deliver a peroxycarboxylic acid product in a form that can be delivered by means that are conventional to (or otherwise suitable in) consumer, industrial, and medical contexts.

In the methods and systems described herein, the cosolvent comprising an organic solvent having a log P of less than about 2, wherein log P is defined as the logarithm of the partition coefficient of a substance between octanol and water, expressed as P=[solute]_(octanol)/[solute]_(water), solubilizes the substrate in the aqueous reaction formulation without substantial loss of perhydrolytic activity of the enzyme catalyst; wherein the cosolvent is not a substrate for said enzyme catalyst.

In some embodiments, the first component comprising the formulation of the enzyme catalyst and the carboxylic acid ester substrate optionally comprises an inorganic or organic buffer, a stabilizer, a corrosion inhibitor, a wetting agent, or combinations thereof. In some embodiments, the source of peroxygen comprises a hydrogen peroxide stabilizer.

As used herein, the term “perhydrolysis” is defined as the reaction of a selected substrate with peroxide to form a peracid. Typically, inorganic peroxide is reacted with the selected substrate in the presence of a catalyst to produce the peracid. As used herein, the term “chemical perhydrolysis” includes perhydrolysis reactions in which a substrate (i.e., a peracid precursor) is combined with a source of hydrogen peroxide wherein peracid is formed in the absence of an enzyme catalyst.

As used herein, the term “perhydrolase activity” refers to the catalyst activity per unit mass (for example, milligram) of protein, dry cell weight, or immobilized catalyst weight.

As used herein, “one unit of enzyme activity” or “one unit of activity” or “U” is defined as the amount of perhydrolase activity required for the production of 1 μmol of peracid product per minute at a specified temperature.

As used herein, the terms “enzyme catalyst” and “perhydrolase catalyst” refer to a catalyst comprising an enzyme having perhydrolysis activity and may be in the form of a whole microbial cell, permeabilized microbial cell(s), one or more cell components of a microbial cell extract, partially purified enzyme, or purified enzyme. The enzyme catalyst may also be chemically modified (e.g., by pegyfation or by reaction with cross-linking reagents). The perhydrolase catalyst may also be immobilized on a soluble or insoluble support using methods well-known to those skilled in the art; see for example, Immobilization of Enzymes and Cells; Gordon F, Bickerstaff, Editor, Humana Press, Totowa, N.J., USA; 1997. As described herein, all of the present enzymes having perhydrolysis activity are structurally members of the carbohydrate esterase family 7 (“CE-7” family) of enzymes (see Coutinho, P. M., Henrissat, B. “Carbohydrate-active enzymes: an integrated database approach” in Recent Advances in Carbohydrate Bioengineering, H. J. Gilbert, G. Davies, B, Henrissat and B. Svensson eds., (1999) The Royal Society of Chemistry, Cambridge, pp. 3-12). The CE-7 family of enzymes has been demonstrated to be particularly effective for producing peracids from a variety of carboxylic acid ester substrates when combined with a source of peroxygen (See PCT publication No. WO2007/070609 and U.S. Patent Application Publication Nos. 2008/0176299, 2008/176783, and 2009/0005590 to DiCosimo at al.; each herein incorporated by reference in their entireties).

Members of the CE-7 family include cephalosporin C deacetylases (CAHs; E.C. 3.1.1.41) and acetyl xylan esterases (AXEs; E.C. 3.1.1.72), Members of the CE-7 esterase family share a conserved signature motif (Vincent et al., supra). Perhydrolases comprising the CE-7 signature motif and/or a substantially similar structure are suitable for use in the present invention. Means to identify substantially similar biological molecules are well known in the art (e.g., sequence alignment protocols, nucleic acid hybridizations, and/or presence of a conserved signature motif). In one aspect, the enzyme catalyst in the present processes and systems comprises a substantially similar enzyme having at feast 30%, preferably at least 33%, more preferably at least 40%, more preferably at least 50%, even more preferably at least 60%, yet even more preferable at least 70%, yet even more preferably at least 80%, yet even more preferably at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% amino acid identity to the sequences provided herein. The nucleic acid molecules encoding the present CE-7 carbohydrate esterases are also provided herein. In further embodiments, the perhydrolase catalyst useful in the present processes and systems is encoded by a nucleic acid molecule that hybridizes under highly stringent conditions to one of the present nucleic acid molecules.

As used herein, the terms “cephalosporin C deacetylase” and “cephalosporin C acetyl hydrolase” refer to an enzyme (E.C. 3.1.1.41) that catalyzes the deacetylation of cephalosporins such as cephalosporin C and 7-aminocephalosporanic acid (Mitsushima et al., (1995) Appl. Env. Microbiol. 61(6):2224-2229). As described herein, several cephalosporin C deacetylases are provided having significant perhydrolysis activity.

As used herein, “acetyl xylan esterases” refers to an enzyme (E.C. 3.1.172; AXEs) that catalyzes the deacetylation of acetylated xylans and other acetylated saccharides. As illustrated herein, several enzymes classified as acetyl xylan esterases are provided having significant perhydrolase activity.

As used herein, the term “Bacillus subtilis ATCC® 31954™” refers to a bacterial cell deposited to the American Type Culture Collection (ATCC®) having international depository accession number ATCC® 31954™. Bacillus subtilis ATCC® 31954™ has been reported to have an ester hydrolase (“diacetinase”) activity capable of hydrolyzing glycerol esters having 2-carbon to 8-carbon acyl groups, especially diacetin (U.S. Pat. No. 4,444,886; herein incorporated by reference in its entirety). As described herein, an enzyme having significant perhydrolase activity has been isolated from B. subtilis ATCC® 31954™ and is provided as SEQ. ID NO: 2. The amino acid sequence of the isolated enzyme has 100% amino acid identity to the cephalosporin C deacetylase provided by GENBANK® Accession No, BAA01729.1 (Mitsushima et al., supra).

As used herein, the term “Bacillus subtilis BE1010” refers to the strain of Bacillus subtilis as reported by Payne and Jackson (J. Bacteriol. 173:2278-2282 (1991)). Bacillus subtilis BE101D is a derivative of Bacillus subtilis subsp. subtilis strain BR151 (ATCC® 33677™) having a chromosomal deletion in the genes encoding subtilisin and neutral protease. As described herein, an enzyme having significant perhydrolase activity has been isolated from B. subtilis BE1010 and is provided as SEQ ID NO: 4. The amino acid sequence of the isolated enzyme has 100% amino acid identity to the cephalosporin deacetylase reported in Bacillus subtilis subsp. subtilis strain 168 (Kunst et al., Nature 390:249-256 (1997)).

As used herein, the term “Bacillus subtilis ATCC® 29233™” refers to a strain of Bacillus subtilis deposited to the American Type Culture Collection (ATCC®) having international depository accession number ATCC® 29233™. As described herein, an enzyme having significant perhydrolase activity has been isolated and sequenced from B. subtilis ATCC® 29233™ and is provided as SEC) ID NO: 26.

As used herein, the term “Clostridium thermocellum ATCC® 27405™” refers to a strain of Clostridium thermocellum deposited to the American Type Culture Collection (ATCC®) having international depository accession number ATCC® 27405™. The amino acid sequence of the enzyme having perhydrolase activity from C. thermocellum ATCC® 27405™ is provided as SEQ ID NO: 12.

As used herein, the term “Bacillus subtilis ATCC® 6633™” refers to a bacterial cell deposited to the American Type Culture Collection (ATCC®) having international depository accession number ATCC® 6633™. Bacillus subtilis ATCC® 6633™ has been reported to have cephalosporin acetylhydrolase activity (U.S. Pat. No. 6,465,233). The amino acid sequence of the enzyme having perhydrolase activity from B. subtilis ATCC® 6633™ is provided as SEQ ID NO: 5.

As used herein, the term “Bacillus licheniformis ATCC®14580™” refers to a bacterial cell deposited to the American Type Culture Collection (ATCC®) having international depository accession number ATCC® 14580™. Bacillus licheniformis ATCC® 14580™ has been reported to have cephalosporin acetylhydrolase activity (GENBANK® YP_(—)077621). The amino acid sequence of the enzyme having perhydrolase activity from B. licheniformis ATCC® 14580™ is provided as SEQ ID NO: 8.

As used herein, the term “Bacillus pumilus PS213” refers to a bacterial cell reported to have acetyl xylan esterase activity (GENBANK® AJ249957). The amino acid sequence of the enzyme having perhydrolase activity from Bacillus pumilus PS213 is provided as SEQ ID NO: 10.

As used herein, the term “Thermotoga neapolitana” refers to a strain of Thermotoga neapolitana reported to have acetyl xylan esterase activity (GENBANK® AAB70869). The amino acid sequence of the enzyme having perhydrolase activity from Thermotoga neapolitana is provided as SEQ ID NO: 14. A variant enzyme derived from SEQ ID NO: 14 has recently been reported having improved perhydrolysis activity and is provided as SEQ ID NO: 73 (See co-owned, co-filed, and copending U.S. patent application Attorney Docket No. CL4392 US NA entitled “IMPROVED PERHYDROLASES FOR ENZYMATIC PERACID GENERATION”; incorporated herein by reference in its entirety), where the Xaa residue at position 277 is Ala, Val, Ser, or Thr.

As used herein, the term “Thermotoga maritima MSB8” refers to a strain of Thermotoga maritima reported to have acetyl xylan esterase activity (GENBANK® NP_(—)227893.1). The amino acid sequence of the enzyme having perhydrolase activity from Thermotoga maritima is provided as SEQ ID NO; 16. A variant enzyme derived from SEQ ID NO: 16 has recently been reported having improved perhydrolysis activity and is provided as SEQ ID NO: 74 (See co-owned, co-filed, and copending U.S. patent application Attorney Docket No. CL4392 US NA), where the Xaa residue at position 277 is Ala, Val, Ser, or Thr.

As used herein, the term “Bacillus clausii KSM-K16” refers to a bacterial cell reported to have cephalosporin-C deacetylase activity (GENBANK® YP_(—)175265). The amino acid sequence of the enzyme having perhydrolase activity from Bacillus clausii KSM-K16 is provided as SEQ ID NO: 24.

As used herein, the term “Thermoanearobacterium saccharolyticum” refers to a bacterial strain reported to have acetyl xylan esterase activity (GENBANK® S41858). The amino acid sequence of the enzyme having perhydrolase activity from Thermoanearobacterium saccharolyticum is provided as SEQ ID NO: 54.

As used herein, the term “Thermotoga lettingae” refers to a bacterial cell reported to have acetyl xylan esterase activity (GENBANK® CP000812). The deduced amino acid sequence of the enzyme having perhydrolase activity from Thermotoga lettingae is provided as SEQ ID NO: 60.

As used herein, the term “Thermotoga petrophila” refers to a bacterial cell reported to have acetyl xylan esterase activity (GENBANK® CP000702). The deduced amino acid sequence of the enzyme having perhydrolase activity from Thermotoga lettingae is provided as SEQ ID NO: 64.

As used herein, the term “Thermotoga sp. RQ2” refers to a bacterial cell reported to have acetyl xylan esterase activity (GENBANK® CP000969). Two different acetyl xylan esterases have been identified from Thermotoga sp RQ2 and are referred to herein as “RQ2(a)” (the deduced amino acid sequence provided as SEQ ID NO: 68) and “RQ2(b)” (the deduced amino acid sequence provided as SEQ ID NO: 72).

As used herein, an “isolated nucleic acid molecule” and “isolated nucleic acid fragment” will be used interchangeably and refer to a polymer of RNA or DNA that is single- or double-stranded, optionally containing synthetic, non-natural or altered nucleotide bases. An isolated nucleic acid molecule in the form of a polymer of DNA may be comprised of one or more segments of cDNA, genomic DNA or synthetic DNA.

The term “amino acid” refers to the basic chemical structural unit of a protein or polypeptide. The following abbreviations are used herein to identify specific amino acids:

Three-Letter One-Letter Amino Acid Abbreviation Abbreviation Alanine Ala A Arginine Arg R Asparagine Asn N Aspartic acid Asp D Cysteine Cys C Glutamine Gln Q Glutamic acid Glu E Glycine Gly G Histidine His H Isoleucine Ile I Leucine Leu L Lysine Lys K Methionine Met M Phenylalanine Phe F Proline Pro P Serine Ser S Threonine Thr T Tryptophan Trp W Tyrosine Tyr Y Valine Val V Any amino acid Xaa X (or as defined herein)

As used herein, “substantially similar” refers to nucleic acid molecules wherein changes in one or more nucleotide bases results in the addition, substitution, or deletion of one or more amino acids, but does not affect the functional properties (i.e., perhydrolytic activity) of the protein encoded by the DNA sequence. As used herein, “substantially similar” also refers to an enzyme having an amino acid sequence that is at least 30%, preferably at least 33%, more preferably at least 40%, more preferably at least 50%, even more preferably at least 60%, even more preferably at least 70%, even more preferably at least 80%, yet even more preferably at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to the sequences reported herein wherein the resulting enzyme retains the present functional properties (i.e., perhydrolytic activity). “Substantially similar” may also refer to an enzyme having perhydrolytic activity encoded by nucleic acid molecules that hybridize under highly stringent conditions to the nucleic acid molecules reported herein. It is therefore understood that the invention encompasses more than the specific exemplary sequences.

For example, it is well known in the art that alterations in a gene which result in the production of a chemically equivalent amino acid at a given site, but do not affect the functional properties of the encoded protein are common. For the purposes of the present invention substitutions are defined as exchanges within one of the following five groups:

-   -   1. Small aliphatic, nonpolar or slightly polar residues: Ala,         Ser, Thr (Pro, Gly);     -   2, Polar, negatively charged residues and their amides: Asp,         Asn, Glu, Gln;     -   3. Polar, positively charged residues: His, Arg, Lys;     -   4. Large aliphatic, nonpolar residues: Met, Leu, Ile, Val (Cys);         and     -   5. Large aromatic residues: Phe, Tyr, and Trp.         Thus, a codon for the amino acid alanine, a hydrophobic amino         acid, may be substituted by a codon encoding another less         hydrophobic residue (such as glycine) or a more hydrophobic         residue (such as valine, leucine, or isoleucine). Similarly,         changes which result in substitution of one negatively charged         residue for another (such as aspartic acid for glutamic acid) or         one positively charged residue for another (such as lysine for         arginine) can also be expected to produce a functionally         equivalent product. In many cases, nucleotide changes which         result in alteration of the N-terminal and C-terminal portions         of the protein molecule would also not be expected to alter the         activity of the protein.

Each of the proposed modifications is well within the routine skill in the art, as is determination of retention of biological activity of the encoded products. Moreover, the skilled artisan recognizes that substantially similar sequences are encompassed by the present invention. In one embodiment, substantially similar sequences are defined by their ability to hybridize, under highly stringent conditions (0.1×SSC, 0.1% SDS, 65° C. and washed with 2×SSC, 0.1% SDS followed by 0.1×SSC, 0.1% SDS, 65° C.) with the sequences exemplified herein. In some embodiments, the present methods and systems may include enzymes having perhydrolase activity encoded by isolated nucleic acid molecules that hybridize under stringent conditions to the nucleic acid molecules reported herein. In preferred embodiments, the present methods and systems employ an enzyme having perhydrolase activity encoded by isolated nucleic acid molecule that hybridize under stringent conditions to a nucleic acid molecule having a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO:3, SEQ ID NO:5; SEQ ID NO: 7; SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO: 17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO: 25; SEQ ID NO:29; SEQ ID NO:48, SEQ ID NO: 51, SEQ ID NO: 53, SEQ ID NO: 56, SEQ ID NO: 57, SEQ ID NO: 58, SEQ ID NO: 59, SEQ ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 63, SEQ ID NO: 65, SEQ ID NO: 66, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 70, and SEQ ID NO: 71.

As used herein, a nucleic acid molecule is “hybridizable” to another nucleic acid molecule, such as a cDNA, genomic DNA, or RNA, when a single strand of the first molecule can anneal to the other molecule under appropriate conditions of temperature and solution ionic strength. Hybridization and washing conditions are well known and exemplified in Sambrook, J, and Russell, D., T. Molecular Cloning: A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor (2001). The conditions of temperature and ionic strength determine the “stringency” of the hybridization. Stringency conditions can be adjusted to screen for moderately similar molecules, such as homologous sequences from distantly related organisms, to highly similar molecules, such as genes that duplicate functional enzymes from closely related organisms. Post-hybridization washes typically determine stringency conditions. One set of preferred conditions uses a series of washes starting with 6×SSC, 0.5% SDS at room temperature for 15 min, then repeated with 2×SSC, 0.5% SDS at 45° C. for 30 min, and then repeated twice with 0.2×SSC, 0.5% SDS at 50° C. for 30 min. A more preferred set of conditions uses higher temperatures in which the washes are identical to those above except for the temperature of the final two 30 min washes in 0.2×SSC, 0.5% SDS was increased to 60° C. Another preferred set of stringent hybridization conditions is 0.1×SSC, 0.1% SDS, 65° C. and washed with 2×SSC, 0.1% SDS followed by a final wash of 0.1×SSC, 0.1% SDS, 65° C. with the sequences exemplified herein.

Hybridization requires that the two nucleic acids contain complementary sequences, although depending on the stringency of the hybridization, mismatches between bases are possible. The appropriate stringency for hybridizing nucleic acids depends on the length of the nucleic acids and the degree of complementation, variables well known in the art. The greater the degree of similarity or homology between two nucleotide sequences, the greater the value of Tm for hybrids of nucleic acids having those sequences. The relative stability (corresponding to higher Tm) of nucleic acid hybridizations decreases in the following order: RNA:RNA, DNA:RNA, DNA:DNA. For hybrids of greater than 100 nucleotides in length, equations for calculating Tm have been derived (Sambrook and Russell, supra). For hybridizations with shorter nucleic acids, i.e., oligonucleotides, the position of mismatches becomes more important, and the length of the oligonucleotide determines its specificity (Sambrook and Russell, supra). In one aspect, the length for a hybridizable nucleic acid is at least about 10 nucleotides. Preferably, a minimum length for a hybridizable nucleic acid is at least about 15 nucleotides in length, more preferably at least about 20 nucleotides in length, even more preferably at least 30 nucleotides in length, even more preferably at least 300 nucleotides in length, and most preferably at least 800 nucleotides in length. Furthermore, the skilled artisan will recognize that the temperature and wash solution salt concentration may be adjusted as necessary according to factors such as length of the probe.

As used herein, the term “percent identity” is a relationship between two or more polypeptide sequences or two or more polynucleotide sequences, as determined by comparing the sequences. In the art, “identity” also means the degree of sequence relatedness between polypeptide or polynucleotide sequences, as the case may be, as determined by the match between strings of such sequences. “Identity” and “similarity” can be readily calculated by known methods, including but not limited to those described in: Computational Molecular Biology (Lesk, A. M., ed.) Oxford University Press, NY (1988); Biocomputing: Informatics and Genome Projects (Smith, D. W., ed.) Academic Press, NY (1993); Computer Analysis of Sequence Data, Part I (Griffin, A M., and Griffin, H. G., eds.) Humana Press, NJ (1994); Sequence Analysis in Molecular Biology (von Heinje, G., ed.) Academic Press (1987); and Sequence Analysis Primer (Gribskov, M. and Devereux, J., eds.) Stockton Press, NY (1991). Methods to determine identity and similarity are codified in publicly available computer programs. Sequence alignments and percent identity calculations may be performed using the Megalign program of the LASERGENE bioinformatics computing suite (DNASTAR Inc., Madison, Wis.), the AlignX program of Vector NTI v. TO (Informax, Inc., Bethesda, Md.), or the EMBOSS Open Software Suite (EMBL-EBI; Rice et al., Trends in Genetics 16, (6) pp 276-277 (2000)). Multiple alignment of the sequences can be performed using the CLUSTAL method (such as CLUSTALW; for example version 1.83) of alignment (Higgins and Sharp, CABIOS, 5:151-153 (1989); Higgins et al., Nucleic Acids Res. 22:4673-4680 (1994); and Chenna et al., Nucleic Acids Res 31 (13):3497-500 (2003)), available from the European Molecular Biology Laboratory via the European Bioinformatics Institute) with the default parameters. Suitable parameters for CLUSTALW protein alignments include GAP Existence penalty=15, GAP extension=0.2, matrix=Gonnet (e.g., Gonnet250), protein ENDGAP=−1, Protein GAPDIST=4, and KTUPLE=1. In one embodiment, a fast or slow alignment is used with the default settings wherein a slow alignment is preferred. Alternatively, the parameters using the CLUSTALW method (version 1.83) may be modified to also use KTUPLE=1, GAP PENALTY=10, GAP extension=1, matrix=BLOSUM (e.g. BLOSUM64), WINDOW=5, and TOP DIAGONALS SAVED=5.

In one aspect of the present methods and systems, suitable isolated nucleic acid molecules (isolated polynucleotides of the present invention) encode a polypeptide having an amino acid sequence that is at least about 30%, preferably at least 33%, preferably at least 40%, preferably at least 50%, preferably at least 60%, more preferably at least 70%, more preferably at feast 80%, even more preferably at least 85%, even more preferably at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to the amino acid sequences reported herein. Suitable nucleic acid molecules of the present invention not only have the above homologies, but also typically encode a polypeptide having about 300 to about 340 amino adds, more preferably about 310 to about 330 amino acids, and most preferably about 318 to about 325 amino acids in length.

As used herein, the terms “signature motif” and “diagnostic motif” refer to conserved structures shared among a family of enzymes having a defined activity. The signature motif can be used to define and/or identify the family of structurally related enzymes having similar enzymatic activity for a defined family of substrates. The signature motif can be a single contiguous amino acid sequence or a collection of discontiguous, conserved motifs that together form the signature motif. Typically, the conserved motif(s) is represented by an amino acid sequence. As described herein, the present perhydrolases belong to the family of CE-7 carbohydrate esterases. This family of enzymes can be defined by the presence of a CE-7 “signature motif” (Vincent et al., supra).

As used herein, “codon degeneracy” refers to the nature of the genetic code permitting variation of the nucleotide sequence without affecting the amino acid sequence of an encoded polypeptide. Accordingly, the present invention relates to any nucleic acid molecule that encodes all or a substantial portion of the amino acid sequences encoding the present microbial polypeptide. The skilled artisan is well aware of the “codon-bias” exhibited by a specific host cell in usage of nucleotide codons to specify a given amino acid. Therefore, when synthesizing a gene for improved expression in a host cell, it is desirable to design the gene such that its frequency of codon usage approaches the frequency of preferred codon usage of the host cell.

As used herein, the term “codon optimized”, as it refers to genes or coding regions of nucleic acid molecules for transformation of various hosts, refers to the alteration of codons in the gene or coding regions of the nucleic acid molecules to reflect the typical codon usage of the host organism without altering the polypeptide for which the DNA codes.

As used herein, “synthetic genes” can be assembled from oligonucleotide building blocks that are chemically synthesized using procedures known to those skilled in the art. These building blocks are ligated and annealed to form gene segments that are then enzymatically assembled to construct the entire gene. “Chemically synthesized”, as pertaining to a DNA sequence, means that the component nucleotides were assembled in vitro. Manual chemical synthesis of DNA may be accomplished using well-established procedures, or automated chemical synthesis can be performed using one of a number of commercially available machines. Accordingly, the genes can be tailored for optimal gene expression based on optimization of nucleotide sequences to reflect the codon bias of the host cell. The skilled artisan appreciates the likelihood of successful gene expression if codon usage is biased towards those codons favored by the host. Determination of preferred codons can be based on a survey of genes derived from the host cell where sequence information is available.

As used herein, “gene” refers to a nucleic acid molecule that expresses a specific protein, including regulatory sequences preceding (5′ non-coding sequences) and following (3′ non-coding sequences) the coding sequence. “Native gene” refers to a gene as found in nature with its own regulatory sequences. “Chimeric gene” refers to any gene that is not a native gene, comprising regulatory and coding sequences that are not found together in nature. Accordingly, a chimeric gene may comprise regulatory sequences and coding sequences that are derived from different sources, or regulatory sequences and coding sequences derived from the same source, but arranged in a manner different from that found in nature. “Endogenous gene” refers to a native gene in its natural location in the genome of an organism. A “foreign” gene refers to a gene not normally found in the host organism, but that is introduced into the host organism by gene transfer. Foreign genes can comprise native genes inserted into a non-native organism, or chimeric genes. A “transgene” is a gene that has been introduced into the genome by a transformation procedure.

As used herein, “coding sequence” refers to a DNA sequence that codes for a specific amino acid sequence. “Suitable regulatory sequences” refer to nucleotide sequences located upstream (5′ non-coding sequences), within, or downstream (3′ non-coding sequences) of a coding sequence, and which influence the transcription, RNA processing or stability, or translation of the associated coding sequence, Regulatory sequences may include promoters, translation leader sequences, RNA processing site, effector binding site and stem-loop structure.

As used herein, “promoter” refers to a DNA sequence capable of controlling the expression of a coding sequence or functional RNA. In general, a coding sequence is located 3′ to a promoter sequence. Promoters may be derived in their entirety from a native gene, or be composed of different elements derived from different promoters found in nature, or even comprise synthetic DNA segments. It is understood by those skilled in the art that different promoters may direct the expression of a gene at different stages of development, or in response to different environmental or physiological conditions. Promoters that cause a gene to be expressed at most times are commonly referred to as “constitutive promoters”. It is further recognized that since in most cases the exact boundaries of regulatory sequences have not been completely defined, DNA fragments of different lengths may have identical promoter activity.

As used herein, the “3′ non-coding sequences” refer to DNA sequences located downstream of a coding sequence and include polyadenylation recognition sequences (normally limited to eukaryotes) and other sequences encoding regulatory signals capable of affecting mRNA processing or gene expression. The polyadenylation signal is usually characterized by affecting the addition of polyadenylic acid tracts (normally limited to eukaryotes) to the 3′ end of the mRNA precursor.

As used herein, the term “operably linked” refers to the association of nucleic acid sequences on a single nucleic acid molecule so that the function of one is affected by the other. For example, a promoter is operably linked with a coding sequence when it is capable of affecting the expression of that coding sequence, i.e., that the coding sequence is under the transcriptional control of the promoter. Coding sequences can be operably linked to regulatory sequences in sense or antisense orientation.

As used herein, the term “expression” refers to the transcription and stable accumulation of sense (mRNA) or antisense RNA derived from the nucleic acid molecule described herein. Expression may also refer to translation of mRNA into a polypeptide.

As used herein, “transformation” refers to the transfer of a nucleic acid molecule into the genome of a host organism, resulting in genetically stable inheritance. In the present invention, the host cell's genome includes chromosomal and extrachromosomal plasmid) genes. Host organisms containing the transformed nucleic acid molecules are referred to as “transgenic” or “recombinant” or “transformed” organisms.

As used herein, the terms “plasmid”, “vector” and “cassette” refer to an extrachromosomal element often carrying genes which are not part of the central metabolism of the cell, and usually in the form of circular double-stranded DNA molecules. Such elements may be autonomously replicating sequences, genome integrating sequences, phage or nucleotide sequences, linear or circular, of a single- or double-stranded DNA or RNA, derived from any source, in which a number of nucleotide sequences have been joined or recombined into a unique construction which is capable of introducing a promoter fragment and DNA sequence for a selected gene product along with appropriate 3′ untranslated sequence into a cell. “Transformation cassette” refers to a specific vector containing a foreign gene and having elements in addition to the foreign gene that facilitate transformation of a particular host cell. “Expression cassette” refers to a specific vector containing a foreign gene and having elements in addition to the foreign gene that allow for enhanced expression of that gene in a foreign host.

As used herein, the term “sequence analysis software” refers to any computer algorithm or software program that is useful for the analysis of nucleotide or amino acid sequences. “Sequence analysis software” may be commercially available or independently developed. Typical sequence analysis software will include, but is not limited to, the GCG suite of programs (Wisconsin Package Version 9.0, Genetics Computer Group (GCG), Madison, Wis.), BLASTP, BLASTN, BLASTX (Altschul et al., J. Mol. Biol. 215:403-410 (1990), and DNASTAR (DNASTAR, Inc. 1228 S. Park St. Madison, Wis. 53715 USA), CLUSTALW (for example, version 1.83; Thompson et al., Nucleic Acids Research, 22(22):4673-4680 (1994), and the FASTA program incorporating the Smith-Waterman algorithm (W. R. Pearson, Cornput. Methods Genome Res., [Proc. Int. Symp.] (1994), Meeting Date 1992, 111-20. Editor(s): Suhai, Sander. Publisher: Plenum, New York, N.Y.), Vector NTI (Informax, Bethesda, Md.) and Sequencher v. 4.05. Within the context of this application it will be understood that where sequence analysis software is used for analysis, that the results of the analysis will be based on the “default values” of the program referenced, unless otherwise specified. As used herein “default values” will mean any set of values or parameters set by the software manufacturer that originally load with the software when first initialized.

As used herein, the term “biological contaminants” refers to one or more unwanted and/or pathogenic biological entities including, but not limited to, microorganisms, spores, viruses, prions, and mixtures thereof. The process produces an efficacious concentration of at least one percarboxylic acid useful to reduce and/or eliminate the presence of the viable biological contaminants. In a preferred embodiment, the biological contaminant is a viable pathogenic microorganism.

As used herein, the term “disinfect” refers to the process of destruction of or prevention of the growth of biological contaminants. As used herein, the term “disinfectant” refers to an agent that disinfects by destroying, neutralizing, or inhibiting the growth of biological contaminants. As used herein, the term “disinfection” refers to the act or process of disinfecting. Typically, disinfectants are used to treat inanimate objects or surfaces. As used herein, the term “antiseptic” refers to a chemical agent that inhibits the growth of disease-carrying biological contaminants (such as microorganisms). In one aspect of the present methods and systems, the biological contaminants are pathogenic microorganisms.

As used herein, the term “sanitary” means of or relating to the restoration or preservation of health, typically by removing, preventing or controlling an agent that may be injurious to health. As used herein, the term “sanitize” means to make sanitary. As used herein, the term “sanitizes” refers to a sanitizing agent. As used herein the term “sanitization” refers to act or process of sanitizing.

As used herein, the term “virucide” refers to an agent that inhibits or destroys viruses, and is synonymous with “viricide”. An agent that exhibits the ability to inhibit or destroy viruses is described as having “virucidal” activity. Peracids can have virucidal activity. Typical alternative virucides known in the art which may be suitable for use with the present invention include, for example, alcohols, ethers, chloroform, formaldehyde, phenols, beta propiolactone, iodine, chlorine, mercury salts, hydroxylamine, ethylene oxide, ethylene glycol, quaternary ammonium compounds, enzymes, and detergents.

As used herein, the term “biocide” refers to a chemical agent, typically broad spectrum, which inactivates or destroys microorganisms. A chemical agent that exhibits the ability to inactivate or destroy microorganisms is described as having “biocidal” activity. Peracids can have biocidal activity. Typical alternative biocides known in the art, which may be suitable for use in the present invention include, for example, chlorine, chlorine dioxide, chloroisocyanurates, hypochlorites, ozone, acrolein, amines, chlorinated phenolics, copper salts, organo-sulphur compounds, and quaternary ammonium salts.

As used herein, the phrase “minimum biocidal concentration” refers to the minimum concentration of a biocidal agent that, for a specific contact time, will produce a desired lethal, irreversible reduction in the viable population of the targeted microorganisms. The effectiveness can be measured by the log₁₀ reduction in viable microorganisms after treatment. In one aspect, the targeted reduction in viable microorganisms after treatment is at least a 3-log reduction, more preferably at least a 4-log reduction, and most preferably at least a 5-log reduction. In another aspect, the minimum biocidal concentration is at least a 6-log reduction in viable microbial cells.

As used herein, the term “benefit agent” refers to something that promotes or enhances a useful advantage or favorable effect. In one embodiment, methods and systems are provided whereby a benefit agent, such as a composition comprising a peroxycarboxylic acid, is applied to an article of clothing or textile to achieve a desired benefit, such as disinfecting, sanitizing, bleaching, stain removal, deodorizing/odor reduction, and any combination thereof.

As used herein, the terms “peroxygen source” and “source of peroxygen” refer to compounds capable of providing hydrogen peroxide at a concentration of about 1 mM or more when in an aqueous solution including, but not limited to hydrogen peroxide, hydrogen peroxide adducts (e.g., urea-hydrogen peroxide adduct (carbamide peroxide)), perborates, and percarbonates. As described herein, the concentration of the hydrogen peroxide provided by the peroxygen compound in the aqueous reaction formulation is initially at least 1 mM or more upon combining the reaction components. In one embodiment, the hydrogen peroxide concentration in the aqueous reaction formulation is at least 10 mM. In another embodiment, the hydrogen peroxide concentration in the aqueous reaction formulation is at least 100 mM. In another embodiment, the hydrogen peroxide concentration in the aqueous reaction formulation is at least 200 mM. In another embodiment, the hydrogen peroxide concentration in the aqueous reaction formulation is 500 mM or more. In yet another embodiment, the hydrogen peroxide concentration in the aqueous reaction formulation is 1000 mM or more. The molar ratio of the hydrogen peroxide to enzyme substrate, e.g. triglyceride, (H₂O₂:substrate) in the aqueous reaction formulation may be from about 0.002 to 20, preferably about 0.1 to 10, and most preferably about 0.5 to 5.

In some embodiments of the presently disclosed methods and systems, the enzyme catalyst comprises a perhydrolase having a structure belonging to the CE-7 carbohydrate esterase family. In other embodiments, the perhydrolase catalyst is structurally classified as a cephalosporin C deacetylase. In other embodiments, the perhydrolase catalyst is structurally classified as an acetyl xylan esterase. The terms “enzyme catalyst”, “enzyme catalyst having perhydrolysis activity”, and “perhydrolase catalyst” are used herein interchangeably.

In some embodiments of the present methods and systems, the perhydrolase catalyst comprises an enzyme having a CE-7 signature motif that aligns with a reference sequence SEQ ID NO: 2 using CLUSTALW, said CE-7 signature motif comprising:

i) an RGQ motif at amino acid positions 118-120 of SEQ ID NO:2;

ii) a GXSQG motif at amino acid positions 179-183 of SEQ ID NO:2; and

iii) an HE motif at amino acid positions 298-299 of SEQ ID NO:2;

wherein said enzyme also comprises at least 30% amino acid identity to SEQ ID NO:2.

In other embodiments of the present methods and systems, the signature motif additional comprises a fourth conserved motif defined as an LXD motif at amino acid residues 267-269 when aligned to reference sequence SEQ ID NO: 2 using CLUSTALW.

In additional embodiments of the present methods and systems, the perhydrolase catalyst may comprise an enzyme having perhydrolase activity selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 54, SEQ ID NO: 60, SEQ ID NO: 64, SEQ ID NO: 68, SEQ ID NO: 72, SEQ ID NO: 73, and SEQ ID NO: 74 or a substantially similar enzyme having perhydrolase activity derived by substituting, deleting or adding one or more amino acids to said amino acid sequence.

In other embodiments of the present methods and systems, substantially similar enzyme having perhydrolase activity is at least 30%, preferably at least 33%, more preferably at least 40%, more preferably at least 50%, even more preferably at least 60%, yet even more preferable at least 70%, yet even more preferably at least 80%, yet even more preferably at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to one or more amino acid sequences selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 54, SEQ ID NO: 60, SEQ ID NO: 64, SEQ ID NO: 68, SEQ ID NO: 72, SEQ ID NO: 73, and SEQ ID NO: 74.

In other embodiments of the present methods and systems, the perhydrolase catalyst comprises an enzyme having an amino acid sequence encoded by a nucleic acid molecule that hybridizes to a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5; SEQ ID NO: 7; SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO: 25; SEQ ID NO:29; SEQ ID NO:48, SEQ ID NO: 51, SEQ ID NO: 53, SEQ ID NO: 56, SEQ ID NO: 57, SEQ ID NO: 58, SEQ ID NO: 59, SEQ ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 63, SEQ ID NO: 65, SEQ ID NO: 66, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 70, and SEQ ID NO: 71 under stringent hybridization conditions.

In other embodiments of the present methods and systems, the perhydrolase catalyst comprises an enzyme having an amino acid sequence selected from the group consisting of SEQ ID NO: 14 and SEQ ID NO: 73 (i.e., the wild type Thermotoga neapolitana and Thermotoga neapolitana variants having an amino acid substitution at amino acid residue 277).

In other embodiments of the present methods and systems, the perhydrolase catalyst comprises an enzyme having an amino acid sequence selected from the group consisting of SEQ ID NO: 16 and SEQ ID NO: 74 (i.e., the wild type Thermotoga maritima and Thermotoga maritima variants having an amino acid substitution at amino acid residue 277).

In other embodiments of the present methods and systems, the perhydrolase catalyst comprises an enzyme having at least 30%, preferably at least 36%, amino acid identity to a contiguous signature motif defined as SEQ ID NO: 49 wherein the conserved motifs described above (i.e., RGQ, GXSQG, and HE, and optionally, LXD) are conserved.

With respect to the presently disclosed methods and systems, suitable carboxylic acid ester substrates include esters provided by the following formula:

[X]_(m)R₅

-   -   wherein X=an ester group of the formula R₆C(O)O

R₆=C1 to C7 linear, branched or cyclic hydrocarbyl moiety, optionally substituted with hydroxyl groups or C1 to C4 alkoxy groups, wherein R₆ optionally comprises one or more ether linkages for R₆=C2 to C7;

R₅=a C1 to C6 linear, branched, or cyclic hydrocarbyl moiety optionally substituted with hydroxyl groups: wherein each carbon atom in R₅ individually comprises no more than one hydroxyl group or no more than one ester group; wherein R₅ optionally comprises one or more ether linkages;

m=1 to the number of carbon atoms in R₅ ⁻, and

wherein said esters have solubility in water of at least 5 ppm at 25° C.

In other embodiments of the present methods and systems, suitable substrates also include esters of the formula:

wherein R₁=C1 to C7 straight chain or branched chain alkyl optionally substituted with a hydroxyl or a C1 to C4 alkoxy group and R₂=C1 to C10 straight chain or branched chain alkyl, alkenyl, alkynyl, aryl, alkylaryl, alkylheteroaryl, heteroaryl, (CH₂CH₂—O)_(n)H or (CH₂CH(CH₃)—O)_(n)H and n=1 to 10.

In other embodiments of the present methods and systems, suitable carboxylic acid ester substrates include glycerides of the formula:

wherein R₁=C1 to C7 straight chain or branched chain alkyl optionally substituted with a hydroxyl or a C1 to C4 alkoxy group and R₃ and R₄ are individually H or R₁C(O).

In other embodiments of the present methods and systems, R₆ is C1 to C7 linear hydrocarbyl moiety, optionally substituted with hydroxyl groups or C1 to C4 alkoxy groups, optionally comprising one or more ether linkages. In further preferred embodiments, R₆ is C2 to C7 linear hydrocarbyl moiety, optionally substituted with hydroxyl groups, and/or optionally comprising one or more ether linkages.

In other embodiments of the disclosed methods and systems, suitable carboxylic acid ester substrates also include acetylated saccharides selected from the group consisting of acetylated mono-, di-, and polysaccharides. In preferred embodiments, the acetylated saccharides include acetylated mono-, di-, and polysaccharides. In other embodiments, the acetylated saccharides are selected from the group consisting of acetylated xylan, fragments of acetylated xylan, acetylated xylose (such as xylose tetraacetate), acetylated glucose (such as glucose pentaacetate), β-D-ribofuranose-1,2,3,5-tetraacetate, tri-O-acetyl-D-galactal, tri-O-acetyl-D-glucal, and acetylated cellulose. In preferred embodiments, the acetylated saccharide is selected from the group consisting of β-D-ribofuranose-1,2,3,5-tetraacetate, tri-O-acetyl-D-galactal, tri-O-acetyl-D-glucal, and acetylated cellulose. As such, acetylated carbohydrates may be suitable substrates for generating percarboxylic acids using the present methods and systems (i.e., in the presence of a peroxygen source).

In additional embodiments of the present methods and systems, the carboxylic acid ester substrate may be monoacetin; triacetin; monopropionin; dipropionin; tripropionin; monobutyrin; dibutyrin; tributyrin; glucose pentaacetate; xylose tetraacetate; acetylated xylan; acetylated xylan fragments; β-D-ribofuranose-1,2,3,5-tetraacetate; tri-O-acetyl-D-galactal; tri-O-acetyl-glucal; propylene glycol diacetate; ethylene glycol diacetate; monoesters or diesters of 1,2-ethanediol; 1,2-propanediol; 1,3-propanediol; 1,2-butanediol; 1,3-butanediol; 2,3-butanediol; 1,4-butanediol; 1,2-pentanediol; 2,5-pentanediol; 1,6-pentanediol; 1,2-hexanediol; 2,5-hexanediol; 1,6-hexanediol; and mixtures thereof. In preferred embodiments of the present methods and systems, the substrate comprises triacetin.

Preferably, the substrate used in the present methods and systems has solubility in water of less than about 100 mg/mL. In some embodiments of the present methods and systems, the percent by weight of the substrate in the aqueous reaction mixture exceeds the solubility limit of the substrate in water.

In other embodiments of the presently disclosed methods and systems, the substrate may be ethyl acetate; methyl lactate; ethyl lactate; methyl glycolate; ethyl glycolate; methyl methoxyacetate; ethyl methoxyacetate; methyl 3-hydroxybutyrate; ethyl 3-hydroxybutyrate; triethyl 2-acetyl citrate; glucose pentaacetate; gluconolactone; glycerides (mono-, di-, and triglycerides) such as monoacetin, diacetin, triacetin, monopropionin, dipropionin (glyceryl dipropionate), tripropionin (1,2,3-tripropionylglycerol), monobutyrin, dibutyrin (glyceryl dibutyrate), tributyrin (1,2,3-tributyrylglycerol); acetylated saccharides; and mixtures thereof.

In a further preferred aspect of the present methods and systems, the carboxylic acid ester substrates are selected from the group consisting of monoacetin, diacetin, triacetin, monopropionin, dipropionin, tripropionin, monobutyrin, dibutyrin, tributyrin, ethyl acetate, and ethyl lactate. In yet another aspect, the carboxylic acid ester substrates are selected from the group consisting of diacetin, triacetin, ethyl acetate, and ethyl lactate. In preferred aspects, the carboxylic acid ester is a glyceride selected from the group consisting of monoacetin, diacetin, triacetin, and mixtures thereof.

Use of Cosolvents in a Multi-Component Generation System.

The cosolvent for use in the presently disclosed methods and systems comprises an organic solvent having a log P of less than about 2. In some embodiments, the cosolvent is preferably an alcohol. The cosolvent preferably may comprise tripropylene glycol methyl ether, dipropylene glycol methyl ether, propylene glycol methyl ether, diethylene glycol butyl ether, dipropylene triethyleneglycol, 1,2-propanediol, N-ethyl-2-pyrroldinone, isopropanol, ethanol, ethyl lactate, 1,3-propanediol, or any combination thereof. In some embodiments of the present methods and systems, the cosolvent comprises tripropylene glycol methyl ether.

In the present methods for producing peroxycarboxylic acid in a multicomponent system, the multicomponent system may comprise a first component comprising the catalyst composition comprising a cosolvent, and a second component comprising the source of peroxygen and water. The second component may comprise an optional stabilizer to extend the shelf-life of the hydrogen peroxide. The catalyst, substrate, and the cosolvent may be combined to form the first component prior to combination of the first component with the second component. Likewise, with respect to the disclosed methods for disinfecting a surface, the step of combining the catalyst composition and the source of peroxygen may comprise combining a first component comprising the catalyst composition and the cosolvent with a second component comprising the source of peroxygen and water.

In embodiments of the present methods and systems wherein the multicomponent system comprises a first component comprising the catalyst composition comprising the cosolvent, and a second component comprising the source of peroxygen and water, the first component may be combined with the second component in a ratio of about 1:1 to about 1:10 by weight. In one embodiment, the first component is combined with the second component in a ratio of about 1:9 by weight.

Within the range of a 1:1 to a 1:10 mixture of the first and second components as described above, a concentration of enzyme substrate (“activator”) in the first component and a ratio of the second component to the first component are also chosen such that the substrate is soluble in the final mixture of the first component and the second component. For example, the solubility of triacetin in water is reported as 71.7 g/L (Seelig, Chemische Berichte; 24: 3466 (1891)), the solubility of 1,2-propanediol diacetate is reported to be 1 part in 10 parts water (Wurtz, Annales de Chimie; 55:443 (1859); Justus Liebigs Annalen der Chemie; 105:206 (1858)), and the solubility of tributyrin in water is reported to be 0.015% (volume/volume) (Loskit, Zeitschrift fuer Physikalische Chemie, Stoechiometrie and Verwandtschaftslehre; 134:137 (1928)). Given the wide range of solubilities for the enzyme substrates of the present invention, where the enzyme substrates are described below, the cosolvent added to the first component of the present invention may function to aid in the dissolution of an enzyme substrate with poor solubility in water, in addition to its function as a diluent in the first component to enable the mixing of a desired ratio of the first component and the second component. In one embodiment of the invention, the substrate is soluble in the resulting formulation of the first component and the second component at a concentration of at least 25% (weight/weight). In a second embodiment of the present invention, the substrate is soluble in the resulting formulation of the first component and the second component at a concentration of at least 10% (weight/weight). In a third embodiment of the present invention, the substrate is soluble in the resulting formulation of the first component and the second component at a concentration of at least 5% (weight/weight). In a fourth embodiment of the present invention, the substrate is soluble in the resulting formulation of the first component and the second component at a concentration of at least 2% (weight/weight).

In one embodiment, the cosolvent may be present in the first component in an amount of about 20% to about 80% by weight. The substrate may be present in the first component in an amount of about 10% to about 60% by weight. In some embodiments, the first component may comprise about 55% by weight substrate, about 40% by weight cosolvent, about 0.3% by weight enzyme catalyst, and about 2.5% by weight filler, and the second component may comprise about 95% by weight water, about 1.5% by weight sodium bicarbonate, and about 1% by weight of a source of peroxygen. In other instances, the first component may comprise about 55.5% by weight triacetin, about 41% by weight tripropylene glycol methyl ether, about 0.9% sodium bicarbonate, about 0.3% by weight of a spray dried enzyme powder comprising Thermotoga neapolitana or Thermotoga maritima perhydrolase or a variant derivative of Thermotoga neapolitana or Thermotoga maritima perhydrolase having one or more point mutations that improve the perhydrolysis activity, and about 2.5% by weight fumed silica, and the second component may comprise about 96% by weight water, about 0.2% by weight hydrogen peroxide stabilizer (e.g., (1-hydroxyethylidene)bisphosphonic acid)), and about 3.2% by weight of a solution comprising 30% hydrogen peroxide.

With respect to the present methods and systems, the carboxylic acid ester substrate may be used at a concentration sufficient to produce the desired concentration of peracid upon enzyme-catalyzed perhydrolysis. The carboxylic acid ester need not be completely soluble in the reaction formulation, but preferably has sufficient solubility to permit conversion of the ester by the perhydrolase catalyst to the corresponding peracid. The carboxylic acid ester is present in the aqueous reaction formulation at a concentration of about 0.0005 wt % to about 40 wt % of the reaction formulation, preferably at a concentration of 0.1 wt % to 20 wt % of the aqueous reaction formulation, and more preferably at a concentration of 0.5 wt % to 10 wt % of the aqueous reaction Formulation. The wt % of carboxylic acid ester may be greater than the solubility limit of the carboxylic acid ester. Not all of the added carboxylic acid ester must immediately dissolve in the aqueous reaction formulation, and after an initial mixing of all reaction components, additional continuous or discontinuous mixing is optional.

In the present methods and systems, the peroxygen source may include, but is not limited to, hydrogen peroxide, hydrogen peroxide adducts (such as urea-hydrogen peroxide adduct (carbamide peroxide)), perborate salts and percarbonate salts. The concentration of peroxygen compound in the aqueous reaction formulation may range from 0.0033 wt % to about 50 wt %, preferably from 0.033 wt % to about 40wt %, more preferably from 0.33 wt % to about 30 wt %.

The reaction formulation of the present methods and systems may comprise from about 5 mM to about 250 mM substrate, from about 5 mM to about 250 mM of the source of peroxygen, and from about 0.0001 mg/mL to about 10 mg/mL, preferably about 0.01 mg/mL to about 2.0 mg/mL, of the enzyme catalyst. In such instances, the substrate may comprise triacetin, the source of peroxygen may comprise hydrogen peroxide, and enzyme catalyst may comprise Thermotoga neapolitana or Thermotoga maritima perhydrolase. In another embodiment, the enzyme catalyst may comprise a Thermotoga neapolitana or Thermotoga maritima variant as defined by SEQ ID NO: 73 or SEQ ID NO: 74, where the wild type cysteine at amino acid residue position 277 is substituted with alanine, valine, serine, or threonine.

Many perhydrolase catalysts (such as whole cells, permeabilized whole cells, and partially purified whole cell extracts) have been reported to have catalase activity (EC 1.11.1.6). Catalases catalyze the conversion of hydrogen peroxide into oxygen and water. In one aspect of the presently disclosed methods and systems, the perhydrolysis catalyst lacks catalase activity. In another aspect, a catalase inhibitor is added to the reaction formulation. Examples of catalase inhibitors include, but are not limited to, sodium azide and hydroxylamine sulfate. One of skill in the art can adjust the concentration of catalase inhibitor as needed. The concentration of the catalase inhibitor typically ranges from 0.1 mM to about 1 M; preferably about 1 mM to about 50 mM; more preferably from about 1 mM to about 20 mM. In one aspect, sodium azide concentration typically ranges from about 20 mM to about 60 mM while hydroxylamine sulfate is concentration is typically about 0.5 mM to about 30 mM, preferably about 10 mM.

In other embodiments of the present methods and systems, the enzyme catalyst lacks significant catalase activity or is engineered to decrease or eliminate catalase activity. The catalase activity in a host cell can be down-regulated or eliminated by disrupting expression of the gene(s) responsible for the catalase activity using well known techniques including, but not limited to, transposon mutagenesis, RNA antisense expression, targeted mutagenesis, and random mutagenesis. In some embodiments of the present methods and systems, the gene(s) encoding the endogenous catalase activity are down-regulated or disrupted (i.e., a “knocked-out”). As used herein, a “disrupted” gene is one where the activity and/or function of the protein encoded by the modified gene is no longer present. Means to disrupt a gene are well-known in the art and may include, but are not limited to, insertions, deletions, or mutations to the gene so long as the activity and/or function of the corresponding protein is no longer present. In a further preferred embodiment, the production host is an E. coli production host comprising a disrupted catalase gene selected from the group consisting of katG (SEQ ID NO: 35) and katE (SEQ ID NO: 44). In other embodiments, the production host is an E. coli strain comprising a down-regulation and/or disruption in both katg1 and katE catalase genes. An E. coli strain comprising a double-knockout of katG and katE is provided herein (E. coli strain KLP18).

The catalase negative E. coli strain KLP18 described herein has been demonstrated to be a superior host for large scale (10-L and greater) production of perhydrolase enzymes compared to the catalase negative strain UM2 (E. coli Genetic Stock Center #7156, Yale University, New Haven Conn.), as determined by growth under fermenter conditions. Although both KLP18 and UM2 are catalase-negative strains, UM2 is known to have numerous nutritional auxotrophies, and therefore requires media that is enriched with yeast extract and peptone. Even when employing enriched media for fermentation, UM2 grew poorly and to a limited maximum cell density (OD). In contrast, KLP18 had no special nutritional requirements and grew to high cell densities on mineral media alone or with additional yeast extract.

The concentration of the catalyst in the aqueous reaction formulation of the present methods and systems depends on the specific catalytic activity of the catalyst and is chosen to obtain the desired rate of reaction. The weight of catalyst in perhydrolysis reactions typically ranges from 0.0001 mg to 10 mg per mL of total reaction volume, preferably from 0.010 mg to 2.0 mg per mL. The catalyst may also be immobilized on a soluble or insoluble support using methods well-known to those skilled in the art; see for example, Immobilization of Enzymes and Cells; Gordon F. Bickerstaff, Editor; Humana Press, Totowa, N.J., USA; 1997. The use of immobilized catalysts permits the recovery and reuse of the catalyst in subsequent reactions. The enzyme catalyst may be in the form of whole microbial cells, permeabilized microbial cells, microbial cell extracts, partially-purified or purified enzymes, or mixtures thereof.

In one aspect of the present methods and systems, the concentration of peracid generated by the combination of chemical perhydrolysis and enzymatic perhydrolysis of the carboxylic acid ester is sufficient to provide an effective concentration of peracid for bleaching, sanitization or disinfection at a desired pH. In another aspect, the present methods and systems provide combinations of enzymes and enzyme substrates to produce the desired effective concentration of peracid, where, in the absence of added enzyme, there is a significantly lower concentration of peracid produced. Although there may in some cases be substantial chemical perhydrolysis of the enzyme substrate by direct chemical reaction of inorganic peroxide with the enzyme substrate, there may not be a sufficient concentration of peracid generated to provide an effective concentration of peracid in the desired applications, and a significant increase in total peracid concentration is achieved by the addition of an appropriate perhydrolase catalyst to the reaction formulation.

In connection with the present systems and methods, the concentration of peracid generated (e.g., peracetic acid) by the perhydrolysis of at least one carboxylic acid ester may be at least about 2 ppm, preferably at least 20 ppm, preferably at least 100 ppm, more preferably at least about 200 ppm peracid, more preferably at least 300 ppm, more preferably at least 500 ppm, more preferably at least 700 ppm, more preferably at least about 1000 ppm peracid, most preferably at least 2000 ppm peracid within 10 minutes, preferably within 5 minutes, and most preferably within 1 minute of initiating the perhydrolysis reaction. The product formulation comprising the peracid may be optionally diluted with water, or a solution predominantly comprised of water, to produce a formulation with the desired lower concentration of peracid. In one aspect of the present methods and systems, the reaction time required to produce the desired concentration of peracid is not greater than about two hours, preferably not greater than about 30 minutes, more preferably not greater than about 10 minutes, even more preferably not greater than about 5 minutes, and most preferably in about 1 minute or less. In other aspects of the present methods for disinfecting a surface, a surface, including a hard surface or inanimate object, contaminated with a biological contaminant(s) is contacted with the peracid formed in accordance with the processes described herein within about 1 minute to about 168 hours of combining said reaction components, or within about 1 minute to about 48 hours, or within about 1 minute to 2 hours of combining said reaction components, or any such time interval therein,

The temperature of the reaction is chosen to control both the reaction rate and the stability of the enzyme catalyst activity. The temperature of the reaction may range from just above the freezing point of the reaction formulation (approximately 0° C.) to about 95° C., preferably about 5° C. to about 75° C., with a more preferred range of reaction temperature of from about 5° C. to about 55° C.

The pH of the final reaction formulation containing peracid is from about 2 to about 9, preferably from about 3 to about 8, more preferably from about 5 to about 8, even more preferably about 6 to about 8, and yet even more preferably about 6.5 to about 7.5. In another embodiment, the pH of the reaction formulation is acidic (pH<7). The pH of the reaction, and of the final reaction formulation, may optionally be controlled by the addition of a suitable buffer, including, but not limited to, bicarbonate, citrate, acetate, phosphate, pyrophosphate, methylphosphonate, succinate, malate, fumarate, tartrate, or maleate. The concentration of buffer, when employed, is typically from 0.1 mM to 1.0 M, preferably from 1 mM to 300 mM, most preferably from 10 mM to 100 mM.

In another aspect of the present methods and systems, the enzymatic perhydrolysis product may contain additional components that provide desirable functionality. In a further aspect, the present methods and systems are used in laundry care applications for the treatment of an article of clothing. Examples of additional components include, but are not limited to, buffers, detergent builders, thickening agents, emulsifiers, surfactants, wetting agents, corrosion inhibitors (such as benzotriazole), enzyme stabilizers, and hydrogen peroxide stabilizers (such as metal ion chelating agents). Many of the additional components are well known in the detergent industry (such as those described in U.S. Pat. No. 5,932,532; hereby incorporated by reference). Examples of emulsifiers include, but are not limited to, polyvinyl alcohol or polyvinylpyrrolidone. Examples of thickening agents include, but are not limited to, LAPONITE® RD, corn starch, PVP, CARBOWAX®, CARBOPOL®, CABOSIL®, polysorbate 20, PVA, and lecithin. Examples of buffering systems include, but are not limited to, sodium phosphate monobasic/sodium phosphate dibasic; sulfamic acid/triethanolamine; citric acid/triethanolamine; tartaric acid/triethanolamine; succinic acid/triethanolamine; and acetic acid/triethanolamine. Examples of surfactants include, but are not limited to, (a) non-ionic surfactants such as block copolymers of ethylene oxide or propylene oxide, ethoxylated or propoxylated linear and branched primary and secondary alcohols, and aliphatic phosphine oxides; (b) cationic surfactants such as quaternary ammonium compounds, particularly quaternary ammonium compounds having a C8-C20 alkyl group bound to a nitrogen atom additionally bound to three C1-C2 alkyl groups; (c) anionic surfactants such as alkane carboxylic acids (e.g., C8-C20 fatty acids), alkyl phosphonates, alkane sulfonates (e.g., sodium dodecylsulphate “SDS”) or linear or branched alkyl benzene sulfonates, alkene sulfonates; and (d) amphoteric and zwitterionic surfactants such as aminocarboxylic acids, aminodicarboxylic acids, alkybetaines, and mixtures thereof. Additional components may include fragrances, dyes, stabilizers of hydrogen peroxide (e.g., metal chelators such as 1-hydroxyethylidene-1,1-diphosphonic acid (DEQUEST® 2010, Solutia Inc., St. Louis, Mo. and ethylenediaminetetraacetic acid (EDTA)), TURPINAL® SL (CAS#2809-21-4), DEQUEST® 0520, DEQUEST® 0531, stabilizers of enzyme activity (e.g., polyethylene glycol (PEG)), and detergent builders.

In another aspect of the present methods of disinfecting a surface, the enzymatic perhydrolysis product may be pre-mixed to generate the desired concentration of peroxycarboxylic acid prior to contacting the surface to be disinfected. In another aspect of the present methods of disinfecting a surface, the enzymatic perhydrolysis product is not pre-mixed to generate the desired concentration of peroxycarboxylic acid prior to contacting the surface (such as a hard surface or inanimate object) to be disinfected, but instead, the components of the reaction formulation that generate the desired concentration of percarboxylic acid are contacted with the surface to be disinfected, sanitized, bleached, destained, deodorized or any combination thereof, generating the desired concentration of peroxycarboxylic acid. In some embodiments, the components of the reaction formulation combine or mix at the locus. In some embodiments, the reaction components are delivered or applied to the locus and subsequently mix or combine to generate the desired concentration of peroxycarboxylic acid.

In any embodiment of the present methods of disinfecting a surface or delivering a benefit to an article of clothing or textile (stain removal, order reduction, bleaching, sanitization, and/or disinfection), the aqueous reaction formulation may be applied to the surface by spraying, pouring, sprinkling, wiping, or by any other suitable technique, of which numerous other examples will be appreciated among those skilled in the art.

In Situ Production of Peracids Using a Perhydrolase Catalyst

Cephalosporin C deacetylases (EC. 3.1.1.41; systematic name cephalosporin C acetylhydrolases; CAHs) are enzymes having the ability to hydrolyze the acetyl ester bond on cephalosporins such as cephalosporin C, 7-aminocephalosporanic acid, and 7-(thiophene-2-acetamido)cephalosporanic acid (Abbott, B. and Fukuda, D., Appl. Microbiol. 30(3):413-419 (1975)). CAHs belong to a larger family of structurally related enzymes referred to as the carbohydrate esterase family seven (CE-7; see Coutinho, P. M., Henrissat, B. “Carbohydrate-active enzymes: an integrated database approach” in Recent Advances in Carbohydrate Bioengineering, H. J. Gilbert, G. Davies, B. Henrissat and B. Svensson eds., (1999) The Royal Society of Chemistry, Cambridge, pp. 3-12.)

The CE-7 family includes both CAHs and acetyl xylan esterases (AXEs; E.C. 3.1.1.72). CE-7 family members share a common structural motif and are quite unusual in that they typically exhibit ester hydrolysis activity for both acetylated xylooligosaccharides and cephalosporin C, suggesting that the CE-7 family represents a single class of proteins with a multifunctional deacetylase activity against a range of small substrates (Vincent et al., supra). Vincent et al. describes the structural similarity among the members of this family and defines a signature sequence motif characteristic of the CE-7 family (“the CE-7 signature motif”).

Members of the CE-7 family are found in plants, fungi (e.g., Cephalosporidium acremonium), yeasts (e.g., Rhodosporidium toruloides, Rhodotorula glutinis), and bacteria such as Thermoanaerobacterium sp.; Norcardia lactamdurans, and various members of the genus Bacillus (Politino et al., Appl. Environ. Microbiol., 63(12):4807-4811 (1997); Sakai et al., J. Ferment Bioeng. 85:53-57 (1998); Lorenz, W. and Wiegel, J., J. Bacterial 179:5436-5441 (1997); Cardoza at al., Appl. Microbiol. Biotechnol., 54(3):406-412 (2000); Mitsushima et al., supra, Abbott, B. and Fukuda, D., Appl. Microbial. 30(3):413-419 (1975); Vincent et al., supra, Takami at al., NAR, 28(21):4317-4331 (2000); Rey at al., Genome Biol., 5(10): article 77 (2004); Degrassi et al., Microbiology., 146:1585-1591 (2000); U.S. Pat. No. 6,645,233; U.S. Pat. No. 5,281,525; U.S. Pat. No. 5,338,676; and WO 99/03984. A non-comprehensive list of CE-7 carbohydrate esterase family members having significant homology to SEQ ID NO: 2 are provided in Table 1.

TABLE 1 Examples of CE-7 Enzymes Having Significant Homology to SEQ ID NO: 2. % Amino Source Organism Acid (GENBANK ® Nucleotide Amino Acid Identity to Accession No. of Sequence Sequence SEQ ID the CE-7 enzyme) (SEQ ID NO:) (SEQ ID NO:) NO: 2. Reference B. subtilis  1 2 100 B. subtilis ATCC ® 31954 ™ SHS 0133 Mitsushima et al., supra B. subtilis subsp.  3 4 98 Kunst et al., subtilis str. 168 supra. (NP_388200) WO99/03984 B. subtilis BE1010 Payne and Jackson, J. Bacteriol. 173: 2278-2282 (1991)) B. subtilis  5 6 96 U.S. Pat. No. ATCC ® 6633 6,465,233 (YP_077621.1) B. subtilis 25 26 96 Abbott and ATCC ® 29233 ™ Fukuda, supra B. licheniformis  7 8 77 Rey et al., supra ATCC ® 14580 ™ (YP_077621.1) B. pumilus PS213 9, 48 10 76 Degrassi et al., (CAB76451.2) supra Clostridium 11 12 57 Copeland et al. thermocellum US Dept. of ATCC ® 27405 ™ Energy Joint (ZP_00504991) Genome Institute (JGI- PGF) Direct Submission GENBANK ® ZP_00504991 Thermotoga 13, 29 14 42 See neapolitana GENBANK ® (AAB70869.1) AAB70869.1 Thermotoga 15, 56 16 42 Nelson et al., maritima MSB8 Nature 399 (NP_227893.1) (6734): 323-329 (1999) Bacillus sp. 19 20 40 Siefert et al. NRRL B-14911 J. Craig Venter (ZP_01168674) Institute. Direct Submission Under GENBANK ® ZP_01168674 Thermoanaerobacterium 17 18 37 Lorenz and sp. Wiegel, supra (AAB68821.1) Bacillus 21 22 36 Takami et al., halodurans C-125 supra (NP_244192) Thermoanearobacterium 53 54 35 Lee, Y. E. and saccharolyticum Zeikus, J. G., J (S41858) Gen Microbiol. (1993), 139 Pt 6: 1235-1243 Bacillus clausii 23, 51 24 33 Kobayashi et KSM-K16 al., Appl. (YP_175265) Microbiol. Biotechnol. 43 (3), 473-481 (1995) Thermotoga 57, 58, and 59 60 37 Copeland et al. lettingae US Dept. of (CP000812) Energy Joint Genome Institute Direct Submission GENBANK ® CP000812 Thermotoga 61, 62, and 63 64 41 Copeland et al. Petrophila US Dept. of (CP000702) Energy Joint Genome Institute Direct Submission GENBANK ® CP000702 Thermotoga sp. 65, 66, and 67 68 42 Copeland et al. RQ2 US Dept. of “RQ2(a)” Energy Joint (CP000969) Genome Institute Direct Submission GENBANK ® CP000969 Thermotoga sp. 69, 70, and 71 72 42 Copeland et al. RQ2 US Dept. of “RQ2(b)” Energy Joint (CP000969) Genome Institute Direct Submission GENBANK ® CP000969

The perhydrolases for use in the present methods and systems are preferably all members of the CE-7 carbohydrate esterase family. The enzyme catalyst may comprise Thermotoga neapolitana perhydrolase defined by SEQ ID NO:14. In other embodiments of the present methods and systems, the enzyme catalyst may comprise Thermotoga maritima perhydrolase defined by SEQ ID NO:16. As described by Vincent et al. (supra), members of the family share a common signature motif that is characteristic of this family. A CLUSTALW alignment of the present perhydrolases illustrates that all of the members belong to the CE-7 carbohydrate esterase family (FIG. 1, sheets A-F). A comparison of the overall percent amino acid identity among several of CE-7 perhydrolases is provided in Table 2,

TABLE 2 Percent Amino Acid Identity Between Perhydrolases¹ 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1 100 2 99 100 3 99 99 100 4 96 96 97 100 5 77 76 77 76 100 6 76 76 76 76 68 100 7 57 57 57 56 56 56 100 8 42 43 43 43 43 42 41 100 9 42 43 42 43 43 42 42 72 100 10 42 43 43 43 44 42 43 71 91 100 11 41 43 43 43 45 42 43 71 97 91 100 12 41 42 42 42 43 41 42 71 98 91 97 100 13 37 37 37 36 39 38 38 64 65 67 66 65 100 14 34 36 35 36 35 36 33 36 32 34 34 33 36 100 15 33 34 33 33 32 34 32 30 30 32 31 31 32 34 100 ¹= Percent Identity determined using blast2seq algorithm using BLOSUM62, gap open = 11, gap extension = 1, x_drop = 0, expect = 10, and wordsize = 3. Tatiana A. Tatusova, Thomas L. Madden (1999), “Blast 2 sequences—a new tool for comparing protein and nucleotide sequence”, FEMS Microbial Lett. 174: 247-250

1. B. subtilis ATCC® 31954™

2. B. subtilis BE1010

3. B. subtilis ATCC® 29233™

4. B. subtilis ATCC® 6633™

5. B. licheniformis 14580

6. B. pumilus PS213

7. C. thermocellum ATCC® 27405™

8. Thermotoga sp. RQ2(b)

9. Thermotoga sp. RQ2(a)

10. T. neapolitana

11. T. maritima

12. T. petrophila

13. T. lettingae

14. T. saccharolyticum

15. B. clausii

Although variation is observed in terms of overall percent amino acid identity (i.e., the Clostridium thermocellum ATCC® 27405™ perhydrolase; SEQ ID NO: 12 shares only 57% amino acid identity with the Bacillus subtilis ATCC® 31954™ perhydrolase; SEQ ID NO: 2, while the Bacillus clausii perhydrolase (SEQ ID NO: 24) shares only 33% identity with SEQ ID NO: 2), each of the present perhydrolase enzymes share the CE-7 signature motif. Accordingly, the perhydrolase catalyst of the present invention is an enzyme structurally classified as belonging to the CE-7 carbohydrate esterase family. Each of the present perhydrolase enzymes comprises the CE-7 signature motif.

In one embodiment of the present methods and systems, suitable perhydrolytic enzymes can be identified by the presence of the CE-7 signature motif (Vincent et al., supra). In preferred embodiments, perhydrolases comprising the CE-7 signature motif are identified using a CLUSTALW alignment against the Bacillus subtilis ATCC® 31954™ perhydrolase (SEQ ID NO: 2; the reference sequence used for relative amino acid position numbering). As per the amino acid residue numbering of SEQ ID NO: 2, the CE-7 signature motif comprises 3 conserved motifs defined as: a) Arg118-Gly119-Gln120; b) Gly179-Xaa180-Ser181-Gln182-Gly183; and c) His298-Glu299. Thus, the enzyme catalyst used in the present systems and methods may be comprise three sequence motifs defined as Arg118-Gly119-Gln120; Gly179-Xaa180-Ser181-Gln182-Gly183; and His298-Glu299 relative to SEQ ID NO:2. Alignments of the respective signature motifs are provided in Table 3. Typically, the Xaa at amino acid residue position 180 is glycine, alanine, proline, tryptophan, or threonine. Two of the three amino acid residues belonging to the catalytic triad are in bold. In one embodiment, the Xaa at amino acid residue position 180 is selected from the group consisting of glycine, alanine, proline, tryptophan, and threonine.

Further analysis of the conserved motifs within the GE-7 carbohydrate esterase family indicates the presence of an additional motif (LXD at amino acid positions 267-269 of SEQ ID NO: 2) that may be used to further define a perhydrolase belonging to the CE-7 carbohydrate esterase family. In a further embodiment of the present methods and systems, the signature motif defined above may include a fourth conserved motif defined as Leu267-Xaa268-Asp269 relative to reference SEQ ID NO: 2. The Xaa at amino acid residue position 268 is typically isoleucine, valine, or methionine. The fourth motif includes the aspartic acid residue that is the third member of the catalytic triad (Ser181-Asp269-His298).

Any number of well-known global alignment algorithms may be used to align two or more amino acid sequences (representing enzymes having perhydrolase activity) to determine the existence of the present signature motif (for example, CLUSTALW or Needleman and Wunsch (J. Mol. Biol., 48:443-453 (1970)). The aligned sequence(s) is compared to the reference sequence (SEQ ID NO: 2). In one embodiment, a CLUSTAL alignment (e.g., CLUSTALW; for example version 1.83)) using a reference amino acid sequence (as used herein the CAH sequence (SEQ ID NO: 2) from the Bacillus subtilis ATCC® 31954™) is used to identify perhydrolases belonging to the CE-7 esterase family. The relative numbering of the conserved amino acid residues is based on the residue numbering of the reference amino acid sequence to account for small insertions or deletions (typically 5 amino acids or less) within the aligned sequence.

A comparison of the overall percent identity among perhydrolases exemplified herein indicates that enzymes having as little as 33% identity to SEQ ID NO: 2 (while retaining the signature motif) exhibit significant perhydrolase activity and are structurally classified as CE-7 carbohydrate esterases. In some embodiments of the present methods and systems, the present perhydrolases include enzymes comprising the present signature motif and at least 30%, preferably at least 33%, more preferably at least 40%, even more preferably at least 42%, even more preferably at least 50%, even more preferably at least 60%, even more preferably at least 70%, even more preferably at least 80%, even more preferably at least 90%, and most preferably at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% amino acid identity to SEQ ID ND: 2.

Examples of CE-7 enzymes comprised of the above signature motif are provided in Table 3.

TABLE 3 Conserved motifs from several CE-7 carbohydrate esterases. GXSQG HE motif^(a) Perhydrolase RGQ motif^(a) motif^(a) LXD motif^(b) (Residue Sequence (Residue #s) (Residue #s) (Residue #s) #s) SEQ ID NO: 2 118-120 179-183 267-269 298-299 SEQ ID NO: 4 118-120 179-183 267-269 298-299 SEQ ID NO: 6 118-120 179-183 267-269 298-299 SEQ ID NO: 8 119-121 180-184 268-270 299-300 SEQ ID NO: 10 118-120 179-183 267-269 298-299 SEQ ID NO: 12 119-121 181-185 269-271 300-301 SEQ ID NO: 14 118-120 186-190 272-274 303-304 SEQ ID NO: 16 118-120 186-190 272-274 303-304 SEQ ID NO: 18 117-119 180-184 270-272 301-302 SEQ ID NO: 20 118-120 178-182 267-269 304-305 SEQ ID NO: 22 118-119 181-185 271-273 302-303 SEQ ID NO: 24 117-119 180-184 270-272 301-302 SEQ ID NO: 26 118-120 179-183 267-269 298-299 SEQ ID NO: 54 117-119 180-184 270-272 301-302 SEQ ID NO: 60 118-120 186-190 272-274 303-304 SEQ ID NO: 64 118-120 186-190 272-274 303-304 SEQ ID NO: 68 118-120 186-190 272-274 303-304 RQ2(a) SEQ ID NO: 72 119-121 187-191 273-275 304-305 RQ2(b) SEQ ID NO: 73 118-120 186-190 272-274 303-304 SEQ ID NO: 74 118-120 186-190 272-274 303-304 ^(a)= Conserved motifs defined by Vincent et al., supra used to define the signature motif. ^(b)= an additional motif that may be useful in further defining the signature motif defined by Vincent et al., supra.

Alternatively, a contiguous signature motif (SEQ ID NO: 49) comprising the 3 conserved motifs identified by Vincent of at, supra, (RGQ, GXSQG, and HE; Amino acids residues 118-299 of SEQ ID NO: 2; an optional 4^(th) motif, LXD, is also underlined) may also be used as a contiguous signature motif to identify CE-7 carbohydrate esterases (FIG. 1, panels A-F). As such, suitable enzymes expected to have perhydrolase activity may also be identified as having at least 30% amino acid identify, preferably at least 36%, more preferably at least 40%, even more preferably at least 50%, yet more preferably at least 60%, yet even more preferably at least 70%, yet even more preferably at least 80%, yet even more preferably at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% amino acid identity to SEQ ID NO: 49 (the 4 conserved motifs found in CE-7 carbohydrate esterases are underlined).

(SEQ ID NO: 49) RGQQSSEDTSISLHGHALGWMTKGILDKDTYYYRGVYLDAVRALEVISS FDEVDETRIGVTGGSQGGGLTIAAAALSDIPKAAVADYPYLSNFERAID VALEQPYLEINSFFRRNGSPETEVQAMKTLSYFDIMNLADRVKVPVLMS IGLIDKVTPPSTVFAAYNHLETEKELKVYRYFGHE.

A comparison using the contiguous signature sequence against several CE-7 esterases having perhydrolase activity is provided in Table 4. BLASTP using default parameters was used.

TABLE 4 Percent Amino Acid Identity of Various CE-7 Carbohydrate Esterases having Perhydrolysis Activity Versus the Contiguous Signature Sequence (SEQ ID NO: 49). Perhydrolase % Identity using E-score Sequence BLASTP (expected) SEQ ID NO: 2 100 3e−92 SEQ ID NO: 4 98 6e−91 SEQ ID NO: 6 98 4e−98 SEQ ID NO: 8 78 1e−78 SEQ IDNO: 10 80 3e−76 SEQ ID NO: 12 63 2e−56 SEQ ID NO: 14 51 1e−41 SEQ ID NO: 16 50 6e−35 SEQ ID NO: 24 36 7e−21 SEQ ID NO: 26 99 2e−90 SEQ ID NO: 54 40 2e−26 SEQ ID NO: 60 40 3e−30 SEQ ID NO: 64 46 6e−35 SEQ ID NO: 68 46 6e−35 SEQ ID NO: 72 48 9e−36

Alternatively, the percent amino acid identity to the complete length of one or more of the perhydrolases for use in the present methods and systems may also be used. Accordingly, suitable enzymes having perhydrolase activity have at least 30%, preferably at least 33%, preferably at least 40%, preferably at least 40%, more preferably at least 50%, more preferably at least 60%, more preferably at least 70%, even more preferably at least 80%, yet even more preferably at least 90%, and most preferably at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% amino acid identity to SEQ ID NO: 2. In further embodiments of the present methods and systems, suitable perhydrolase catalysts comprise an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO: 26, SEQ ID NO: 54, SEQ ID NO: 60, SEQ ID NO: 64, SEQ ID NO: 68, SEQ ID NO: 72, SEQ ID NO: 73, and SEQ ID NO: 74. In another embodiment, the amino acid residue at position 277 of either SEQ ID NO: 73 or SEQ ID NO: 74 is selected from the group consisting of alanine, valine, serine, and threonine. In preferred embodiments, suitable enzymes having perhydrolase activity having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% amino acid identity to SEQ ID NO: 14 or to SEQ ID NO: 16 may be used. In further preferred embodiments, suitable enzymes having perhydrolase activity have an amino acid sequence selected from the group consisting of SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 73, and SEQ ID NO: 74.

Suitable carbohydrate esterase family 7 (CE-7) enzymes having perhydrolysis activity for use in the present methods and systems may also include enzymes having one or more deletions, substitutions, and/or insertions to one of the present perhydrolase enzymes (such as SEQ ID NOs, 14 or 16). As shown in Table 2, CE-7 carbohydrates esterases having perhydrolase activity share as little as 31% overall amino acid identity. Additional enzymes having perhydrolase activity structurally classified as belonging to the CE-7 carbohydrate esterase family may have even lower percent identity, so long as the enzyme retains the conserved signature motif. As such, the numbers of deletions, substitutions, and/or insertions may vary so long as the conserved signature motifs (see Table 3) are found in their relative positions within the enzyme.

Additionally, it is well within one of skill in the art to identity suitable enzymes according to the structural similarity found within the corresponding nucleic acid sequence. Hybridization techniques can be used to identity similar gene sequences. Accordingly, suitable perhydrolase catalysts of the present invention comprise an amino acid sequence encoded by a nucleic acid molecule that hybridizes under highly stringent conditions to a nucleic acid molecule having a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO:3, SEQ ID NO:5; SEQ ID NO: 7; SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO: 17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO: 25; SEQ ID NO:29; SEQ ID NO:48, SEQ ID NO: 51, SEQ ID NO: 53, SEQ ID NO: 56, SEQ ID NO: 57, SEQ ID NO: 58, SEQ ID NO: 59, SEQ ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 63, SEQ ID NO: 65, SEQ ID NO: 66, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 70, and SEQ ID NO: 71.

Several variant CE-7 enzymes have recently been identified having enhanced perhydrolysis activity relative to the corresponding wild-type enzymes from which they were derived (see co-owned, co-filed, and copending U.S. patent application having attorney docket number CL4392 US NA; herein incorporated by reference). Specifically, several wild-type CE-7 enzymes from the genus Thermotoga were modified to produce several variants having enhanced perhydrolysis activity. Sequences of the variants are provided as SEQ ID NOs: 73 and 74 wherein the amino acid residue at position 277 of either SEQ ID NO: 73 or SEQ ID NO: 74 is selected from the group consisting of alanine, valine, serine, and threonine.

Enzymatic Multi-Component Peracid Generation Systems

The present methods and systems may be used in the production of industrially useful, efficacious concentrations of peracids in situ under aqueous reaction conditions using the perhydrolase activity of an enzyme belonging to carbohydrate esterase family 7. In some embodiments, the enzyme having perhydrolase activity is also classified structurally and functionally as a cephalosporin C deacetylase (CAH). In other embodiments, the enzyme having perhydrolase activity is classified structurally and functionally as an acetyl xylan esterase (AXE).

The peracids produced in accordance with the present methods and systems are quite reactive and may decrease in concentration over extended periods of time, depending on variables including, but are not limited to, temperature and pH. As such, it may be desirable to keep the various reaction components separated, especially for liquid formulations. In one aspect, the hydrogen peroxide source is separate from either the substrate or the perhydrolase catalyst, preferably from both. This can be accomplished using a variety of techniques including, but not limited to, the use of multi-compartment chambered dispensers (such as U.S. Pat. No. 4,585,150) and at the time of use physically combining the perhydrolase catalyst with an inorganic peroxide and the present substrates to initiate the aqueous enzymatic perhydrolysis reaction. The perhydrolase catalyst may optionally be immobilized within the body of reaction chamber or separated (such as filtered) from the reaction product comprising the peracid prior to contacting the surface and/or object targeted for treatment. The perhydrolase catalyst may be in a liquid matrix or in a solid form (such as a powder or tablet) or embedded within a solid matrix that is subsequently mixed with the substrates to initiate the enzymatic perhydrolysis reaction. In a further aspect, the perhydrotase catalyst may be contained within a dissolvable or porous pouch that may be added to the aqueous substrate matrix to initiate enzymatic perhydrolysis. In additional further aspects, a powder comprising the enzyme catalyst is suspended in the carboxylic acid ester substrate (such as triacetin) and at time of use is mixed with a source of peroxygen in water. In some embodiments, a two-compartment spray bottle such as a dual-liquid fixed ratio sprayer (Model DLS100, Take 5 Corp., Rogue River, Oreg.) or a dual-liquid variable ratio sprayer (Model DLS200, Take; 5 Corp.) is utilized (U.S. Pat. No. 5,152,461 and U.S. Pat. No. 5,532,157; herein incorporated by reference). In another embodiment, a single bottle containing two separate compartments separated by a breakable seal is employed. In some embodiments, the ratio of the volume of the two separate compartments is 1:1, or 5:1 or 10:1. Examples of multi-component delivery systems may also be found in co-owned, co-filed, and copending U.S. patent application Attorney Docket No. CL4400 US NA (incorporated herein by reference in its entirety).

The presently disclosed systems for producing peroxycarboxylic acid may comprise a first component comprising an enzyme catalyst (such as an enzyme powder), a carboxylic acid ester substrate (substantially free of water), and the cosolvent, and a second component comprising the source of peroxygen and water. The presence of excess water in the first component may lead to storage instability. As such, the phrase “substantially free of water” will refer to a concentration of water in the component comprising the carboxylic acid ester substrate/enzyme/cosolvent that does not adversely impact the storage stability of enzyme when present in the first component. In one embodiment, “substantially free of water” may mean less than 2000 ppm, preferably less than 1000 ppm, more preferably less than 500 ppm, and even more preferably less than 250 ppm of water in the carboxylic acid ester substrate containing component. In such embodiments, the systems may further comprise a first vessel/container for storing the first component and a second vessel/container for storing the second component. As used herein, the term “container” may be used generically to describe vessels, compartments, bottles, packets, and other packing systems suitable for holding and/or transporting the present materials. The present systems may also comprise a mixing compartment for receiving at least some of the first component from the first container and at least some of the second component from the second container, thereby permitting the formation of a formulation comprising at least some of the first component and at least some of the second component. In such instances, the systems may further comprise a nozzle for dispensing the formulation from the mixing compartment.

In other embodiments of the present systems, the system comprising a first and second vessel may further comprise a nozzle for receiving at least some of the first component from the first vessel and at least some of the second component from the second vessel, and for dispensing at least some of the first component contemporaneously with at least some of the second component. As used herein, “contemporaneously” means that during at least part of the time that at least some of the first component is dispensed, at least some of the second component is also dispensed. Thus, where some of the first component is dispensed for a total duration of one second, dispensing the second component for 1 second after dispensing the first component and for 0.1 seconds during the dispensing of the first component will be considered to have been contemporaneous with the dispensing of the first component.

Methods for Determining the Concentration of Peracid and Hydrogen Peroxide.

A variety of analytical methods may be used in the present methods to analyze the reactants and products including, but not limited to, titration, high performance liquid chromatography (HPLC), gas chromatography (GC), mass spectroscopy (MS), capillary electrophoresis (CE), the analytical procedure described by U. Karst et al., (Anal. Chem., 69(17)3623-3627 (1997)), and the 2,2′-azino-bis(3-ethylbenzothazoline)-6-sulfonate (ABTS) assay (S. Minning, et al., Analytica Chimica Acta 378:293-298 (1999) and WO 2004/058961 A1) as described in the present examples.

Determination of Minimum Biocidal Concentration of Peracids

The method described by Gabrielson, of al. (J. Microbiol. Methods 50: 63-73 (2002)) may be employed for determination of the Minimum Biocidal Concentration (MBC) of peracids, or of hydrogen peroxide and enzyme substrates. The assay method is based on XTT reduction inhibition, where XTT ((2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-5-[(phenylamino)carbonyl]-2H-tetrazolium, inner salt, monosodium salt) is a redox dye that indicates microbial respiratory activity by a change in optical density (OD) measured at 490 nm or 450 nm. However, there are a variety of other methods available for testing the activity of disinfectants and antiseptics including, but not limited to, viable plate counts, direct microscopic counts, dry weight, turbidity measurements, absorbance, and bioluminescence (see, for example Brock, Semour S., Disinfection, Sterilization, and Preservation, 5^(th) edition, Lippincott Williams & Wilkins, Philadelphia, Pa., USA; 2001).

Uses of Enzymatically Prepared Peracid Compositions

The enzyme catalyst-generated peroxycarboxylic acid produced according to the present method can be used in a variety of hard surface/inanimate object applications for reduction of concentrations of biological contaminants, such as decontamination of medical instruments (e.g., endoscopes), textiles (e.g., garments, carpets), food preparation surfaces, food storage and food-packaging equipment, materials used for the packaging of food products, chicken hatcheries and grow-out facilities, animal enclosures, and spent process waters that have microbial and/or virucidal activity. The enzyme-generated peroxycarboxylic acids may be used in formulations designed to inactivate prions (e.g., certain proteases) to additionally provide biocidal activity. In a preferred aspect, the present peroxycarboxylic acid compositions are particularly useful as a disinfecting agent for non-autoclavable medical instruments and food packaging equipment. As the peroxycarboxylic acid-containing formulation may be prepared using GRAS or food-grade components (enzyme, enzyme substrate, hydrogen peroxide, and buffer), the enzyme-generated peroxycarboxylic acid may also be used for decontamination of animal carcasses, meat, fruits and vegetables, or for decontamination of prepared foods. The enzyme-generated peroxycarboxylic acid may be incorporated into a product whose final form is a powder, liquid, gel, film, solid or aerosol. The enzyme-generated peroxycarboxylic acid may be diluted to a concentration that still provides an efficacious decontamination.

The compositions comprising an efficacious concentration of peroxycarboxylic acid can be used to disinfect surfaces and/or objects contaminated (or suspected of being contaminated) with biological contaminants by contacting the surface or object with the products produced by the present processes. As used herein, “contacting” refers to placing a disinfecting composition comprising an effective concentration of peroxycarboxylic acid in contact with the surface or inanimate object suspected of contamination with a biological contaminant for a period of time sufficient to clean and disinfect. Contacting includes spraying, treating, immersing, flushing, pouring on or in, mixing, combining, painting, coating, applying, affixing to and otherwise communicating a peroxycarboxylic acid solution or composition comprising an efficacious concentration of peroxycarboxylic acid, or a solution or composition that forms an efficacious concentration of peroxycarboxylic acid, with the surface or inanimate object suspected of being contaminated with a concentration of a biological contaminant. The disinfectant compositions may be combined with a cleaning composition to provide both cleaning and disinfection. Alternatively, a cleaning agent (e.g., a surfactant or detergent) may be incorporated into the formulation to provide both cleaning and disinfection in a single composition.

The compositions comprising an efficacious concentration of peroxycarboxylic acid can also contain at least one additional antimicrobial agent, combinations of prion-degrading proteases, a virucide, a sporicide, or a biocide. Combinations of these agents with the peroxycarboxylic acid produced by the claimed processes can provide for increased and/or synergistic effects when used to clean and disinfect surfaces and/or objects contaminated (or suspected of being contaminated) with biological contaminants. Suitable antimicrobial agents include carboxylic esters (e.g., p-hydroxy alkyl benzoates and alkyl cinnamates); sulfonic acids (e.g., dodecylbenzene sulfonic acid); iodo-compounds or active halogen compounds (e.g., elemental halogens, halogen oxides (e.g., NaOCl, HOCl, HOBr, ClO₂), iodine, interhalides (e.g., iodine monochloride, iodine dichloride, iodine trichloride, iodine tetrachloride, bromine chloride, iodine monobromide, or iodine dibromide), polyhalides, hypochlorite salts, hypochlorous acid, hypobromite salts, hypobromous acid, chloro- and bromo-hydantoins, chlorine dioxide, and sodium chlorite); organic peroxides including benzoyl peroxide, alkyl benzoyl peroxides, ozone, singlet oxygen generators, and mixtures thereof; phenolic derivatives (such as o-phenyl phenol, o-benzyl-p-chlorophenol, tent-amyl phenol and C₁-C₆ alkyl hydroxy benzoates); quaternary ammonium compounds (such as alkyldimethylbenzyl ammonium chloride, dialkyldimethyl ammonium chloride and mixtures thereof); and mixtures of such antimicrobial agents, in an amount sufficient to provide the desired degree of microbial protection. Effective amounts of antimicrobial agents include about 0.001 wt % to about 60 wt % antimicrobial agent, about 0.01 wt % to about 15 wt % antimicrobial agent, or about 0.08 wt % to about 2.5 wt % antimicrobial agent.

In one aspect, the peroxycarboxylic acids formed by the present process can be used to reduce the concentration of viable biological contaminants (such as a viable microbial population) when applied on and/or at a locus. As used herein, a “locus” comprises part or all of a target surface suitable for disinfecting or bleaching. Target surfaces include all surfaces that can potentially be contaminated with biological contaminants. Non-limiting examples include equipment surfaces found in the food or beverage industry (such as tanks, conveyors, floors, drains, coolers, freezers, equipment surfaces, walls, valves, belts, pipes, drains, joints, crevasses, combinations thereof, and the like); building surfaces (such as wails, floors and windows); non-food-industry related pipes and drains, including water treatment facilities, pools and spas, and fermentation tanks; hospital or veterinary surfaces (such as walls, floors, beds, equipment (such as endoscopes), clothing worn in hospital/veterinary or other healthcare settings, including clothing, scrubs, shoes, and other hospital or veterinary surfaces); restaurant surfaces; bathroom surfaces; toilets; clothes and shoes; surfaces of barns or stables for livestock, such as poultry, cattle, dairy cows, goats, horses and pigs; hatcheries for poultry or for shrimp; and pharmaceutical or biopharmaceutical surfaces (e.g., pharmaceutical or biopharmaceutical manufacturing equipment, pharmaceutical or biopharmaceutical ingredients, pharmaceutical or biopharmaceutical excipients). Additional hard surfaces also include food products, such as beef, poultry, pork, vegetables, fruits, seafood, combinations thereof, and the like. The locus can also include water absorbent materials such as infected linens or other textiles. The locus also includes harvested plants or plant products including seeds, corms, tubers, fruit, and vegetables, growing plants, and especially crop growing plants, including cereals, leaf vegetables and salad crops, root vegetables, legumes, berried fruits, citrus fruits and hard fruits.

Non-limiting examples of hard surface materials are metals (e.g., steel, stainless steel, chrome, titanium, iron, copper, brass, aluminum, and alloys thereof), minerals (e.g., concrete), polymers and plastics (e.g., polyolefins, such as polyethylene, polypropylene, polystyrene, poly(meth)acrylate, polyacrylonitrile, polybutadiene, poly(acrylonitrile, butadiene, styrene), poly(acrylonitrile, butadiene), acrylonitrile butadiene; polyesters such as polyethylene terephthalate; and polyamides such as nylon). Additional surfaces include brick, tile, ceramic, porcelain, wood, vinyl, linoleum, and carpet.

The peroxycarboxylic acids formed by the present process may be used to provide a benefit to a textile including, but not limited to, bleaching, destaining, sanitization, disinfection, and deodorizing. The peroxycarboxylic acids formed by the present process may be used in any number of laundry care products including, but not limited to, textile pre-wash treatments, laundry detergents, stain removers, bleaching compositions, deodorizing compositions, and rinsing agents.

Recombinant Microbial Expression

With respect to the present methods and systems, the genes and gene products of the sequences described herein may be produced in heterologous host cells, particularly in the cells of microbial hosts. Preferred heterologous host cells for expression of the genes and nucleic acid molecules are microbial hosts that can be found within the fungal or bacterial families and which grow over a wide range of temperature, pH values, and solvent tolerances. For example, it is contemplated that any of bacteria, yeast, and filamentous fungi may suitably host the expression of the present nucleic acid molecules. The perhydrolase may be expressed intracellularly, extracellularly, or a combination of both intracellularly and extracellularly, where extracellular expression renders recovery of the desired protein from a fermentation product more facile than methods for recovery of protein produced by intracellular expression. Transcription, translation and the protein biosynthetic apparatus remain invariant relative to the cellular feedstock used to generate cellular biomass; functional genes will be expressed regardless. Examples of host strains include, but are not limited to, bacterial, fungal or yeast species such as Aspergillus, Trichoderma, Saccharomyces, Pichia, Phaffia, Candida, Hansenula, Yarrowia, Kluyveromyces, Salmonella, Bacillus, Acinetobacter, Zymomonas, Agrobacterium, Erythrobacter, Chlorobium, Chromatium, Flavobacterium, Cytophaga, Rhodobacter, Rhodococcus, Streptomyces, Brevibacterium, Corynebacteria, Mycobacterium, Deinococcus, Escherichia, Erwinia, Pantoea, Pseudomonas, Sphingomonas, Methylomonas, Methylobacter, Methylococcus, Methylosinus, Methylomicrobium, Methylocystis, Alcaligenes, Synechocystis, Synechococcus, Anabaena, Thiobaciflus, Methanobacterium, Klebsiella, and Myxococcus. In one embodiment, bacterial host strains include Kluyveromyces, Escherichia, Bacillus, and Pseudomonas. In a preferred embodiment, the bacterial host cell is Escherichia coli.

Large-scale microbial growth and functional gene expression may use a wide range of simple or complex carbohydrates, organic acids and alcohols or saturated hydrocarbons, such as methane or carbon dioxide in the case of photosynthetic or chemoautotrophic hosts, the form and amount of nitrogen, phosphorous, sulfur, oxygen, carbon or any trace micronutrient including small inorganic ions. The regulation of growth rate may be affected by the addition, or not, of specific regulatory molecules to the culture and which are not typically considered nutrient or energy sources.

Vectors or cassettes useful for the transformation of suitable host cells are well known in the art. Typically the vector or cassette contains sequences directing transcription and translation of the relevant gene, a selectable marker, and sequences allowing autonomous replication or chromosomal integration. Suitable vectors comprise a region 5′ of the gene which harbors transcriptional initiation controls and a region 3′ of the DNA fragment which controls transcriptional termination. It is most preferred when both control regions are derived from genes homologous to the transformed host cell and/or native to the production host, although such control regions need not be so derived.

Initiation control regions or promoters, which are useful to drive expression of the present cephalosporin C deacetylase coding region in the desired host cell are numerous and familiar to those skilled in the art. Virtually any promoter capable of driving these genes is suitable for the present invention including but not limited to CYC1, HIS3, GAL1, GAL10, ADH1, PGK, PHO5, GAPDH, ADC1, TRP1, URA3, LEU2, ENO, TPI (useful for expression in Saccharomyces); AOX1 (useful for expression in Pichia); and lac, araB, tet, trp, IP_(L), IP_(R), T7, tac, and trc (useful for expression in Escherichia coli) as well as the amy, apr, npr promoters and various phage promoters useful for expression in Bacillus.

Termination control regions may also be derived from various genes native to the preferred host cell. In one embodiment, the inclusion of a termination control region is optional. In another embodiment, the chimeric gene includes a termination control region derived the preferred host cell.

Industrial Production

A variety of culture methodologies may be applied to produce the present perhydrolase catalysts in accordance with the present methods and systems. For example, large-scale production of a specific gene product overexpressed from a recombinant microbial host may be produced by both batch and continuous culture methodologies.

A classical batch culturing method is a closed system where the composition of the media is set at the beginning of the culture and not subject to artificial alterations during the culturing process. Thus, at the beginning of the culturing process, the media is inoculated with the desired organism or organisms and growth or metabolic activity may occur without adding anything further to the system. Typically, however, a “batch” culture is batch with respect to the addition of carbon source, and attempts are often made to control factors such as pH and oxygen concentration. In batch systems the metabolite and biomass compositions of the system change constantly up to the time the culture is terminated. Within batch cultures cells moderate through a static lag phase to a high growth log phase and finally to a stationary phase where growth rate is diminished or halted. If untreated, cells in the stationary phase will eventually die. Cells in log phase are often responsible for the bulk of production of end product or intermediate in some systems. Stationary or post-exponential phase production can be obtained in other systems.

A variation on the standard batch system is the fed-batch system. Fed-batch culture processes are also suitable in the present invention and comprise a typical batch system except that the substrate is added in increments as the culture progresses. Fed-batch systems are useful when catabolite repression is apt to inhibit the metabolism of the cells and where it is desirable to have limited amounts of substrate in the media. Measurement of the actual substrate concentration in fed-batch systems is difficult and is estimated on the basis of the changes of measurable factors such as pH, dissolved oxygen and the partial pressure of waste gases such as CO₂. Batch and fed-batch culturing methods are common and well known in the art and examples may be found in Thomas D. Brock in Biotechnology: A Textbook of Industrial Microbiology, Second Edition, Sinauer Associates, Inc., Sunderland, Mass. (1989) and Deshpande, Mukund V., Appl. Biochem. Biotechnol., 38:227-234 (1992).

Commercial production of the desired perhydrolase catalysts may also be accomplished with a continuous culture. Continuous cultures are an open system where a defined culture media is added continuously to a bioreactor and an equal amount of conditioned media is removed simultaneously for processing. Continuous cultures generally maintain the cells at a constant high liquid phase density where cells are primarily in log phase growth. Alternatively, continuous culture may be practiced with immobilized cells where carbon and nutrients are continuously added and valuable products, by-products or waste products are continuously removed from the cell mass. Cell immobilization may be performed using a wide range of solid supports composed of natural and/or synthetic materials.

Continuous or semi-continuous culture allows for the modulation of one factor or any number of factors that affect cell growth or end product concentration. For example, one method will maintain a limiting nutrient such as the carbon source or nitrogen level at a fixed rate and allow all other parameters to moderate. In other systems a number of factors affecting growth can be altered continuously while the cell concentration, measured by media turbidity, is kept constant. Continuous systems strive to maintain steady state growth conditions, and thus the cell loss due to media being drawn off must be balanced against the cell growth rate in the culture. Methods of modulating nutrients and growth factors for continuous culture processes as well as techniques for maximizing the rate of product formation are well known in the art of industrial microbiology and a variety of methods are detailed by Brock, supra.

Fermentation media in the present invention must contain suitable carbon substrates. Suitable substrates may include, but are not limited to, monosaccharides such as glucose and fructose, disaccharides such as lactose or sucrose, polysaccharides such as starch or cellulose or mixtures thereof and unpurified mixtures from renewable feedstocks such as cheese whey permeate, cornsteep liquor, sugar beet molasses, and barley malt. Additionally, the carbon substrate may also be one-carbon substrates such as carbon dioxide, methane or methanol (for example, when the host cell is a methylotrophic microorganism). Similarly, various species of Candida will metabolize alanine or oleic acid (Sulter et al., Arch. Microbiol., 153:485-489 (1990)). Hence, it is contemplated that the source of carbon utilized in the present invention may encompass a wide variety of carbon-containing substrates and will only be limited by the choice of organism,

Recovery of the desired perhydrolase catalysts from a batch fermentation, fed-batch fermentation, or continuous culture, may be accomplished by any of the methods that are known to those skilled in the art. For example, when the enzyme catalyst is produced intracellularly, the cell paste is separated from the culture medium by centrifugation or membrane filtration, optionally washed with water or an aqueous buffer at a desired pH, then a suspension of the cell paste in an aqueous buffer at a desired pH is homogenized to produce a cell extract containing the desired enzyme catalyst. The cell extract may optionally be filtered through an appropriate filter aid such as celite or silica to remove cell debris prior to a heat-treatment step to precipitate undesired protein from the enzyme catalyst solution. The solution containing the desired enzyme catalyst may then be separated from the precipitated cell debris and protein by membrane filtration or centrifugation, and the resulting partially-purified enzyme catalyst solution concentrated by additional membrane filtration, then optionally mixed with an appropriate carrier (for example, maltodextrin, phosphate buffer, citrate buffer, or mixtures thereof) and spray-dried to produce a solid powder comprising the desired enzyme catalyst.

Applicants specifically incorporate the entire contents of all cited references in this disclosure. Further, when an amount, concentration, or other value or parameter is given either as a range, preferred range, or a list of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. Where a range of numerical values is recited herein, unless otherwise stated, the range is intended to include the endpoints thereof, and all integers and fractions within the range. It is not intended that the scope be limited to the specific values recited when defining a range.

GENERAL METHODS

The following examples are provided to demonstrate preferred aspects of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the present inventors to function well in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the present invention.

All reagents and materials were obtained from DIFCO Laboratories (Detroit, Mich.), GIBCO/BRL (Gaithersburg, Md.), TCI America (Portland, Oreg.), Roche Diagnostics Corporation (Indianapolis, Ind.) or Sigma/Aldrich Chemical Company (St. Louis, Mo.), unless otherwise specified.

The following abbreviations in the specification correspond to units of measure, techniques, properties, or compounds as follows: “sec” or “s” means second(s), “min” means minute(s), “h” or “hr” means hour(s), “μL” means microliter(s), “mL” means milliliter(s), “L” means liter(s), “mM” means millimolar, “M” means molar, “mmol” means millimole(s), “ppm” means part(s) per million, “wt” means weight, “wt %” means weight percent, “g” means gram(s), “mg” means milligram(s), “μg” means microgram(s), “ng” means nanogram(s), “g” means gravity, “HPLC” means high performance liquid chromatography, “dd H₂O” means distilled and deionized water, “dcw” means dry cell weight, “ATCC” or “ATCC®” means the American Type Culture Collection (Manassas, Va.), “U” means unit(s) of perhydrolase activity, “rpm” means revolution(s) per minute, and “EDTA” means ethylenediaminetetraacetic acid.

Example 1

Construction of a katG Catalase Disrupted E. coli Strain

The coding region of the kanamycin resistance gene (kan; SEQ ID NO: 30) was amplified from the plasmid pKD13 (SEQ ID NO: 31) by PCR (0.5 min at 94° C., 0.5 min at 55° C., 1 min at 70° C., 30 cycles) using primers identified as SEQ ID NO: 32 and SEQ ID NO: 33 to generate the PCR product identified as SEQ ID NO: 34. The katG nucleic acid sequence is provided as SEQ ID NO: 35 and the corresponding amino acid sequence is SEQ ID NO: 36. E. coli MG1655 (ATCC® 47076™) was transformed with the temperature-sensitive plasmid pKD46 (SEQ ID NO: 37), which contains the λ-Red recombinase genes (Datsenko and Wanner, 2000, PAPAS USA 97:6640-6645), and selected on LB-amp plates for 24 h at 30° C. MG1655/pKD46 was transformed with 50-500 ng of the PCR product by electroporation (BioRad Gene Pulser, 0.2 cm cuvette, 2.5 kV, 200 W, 25 μF), and selected on LB-kan plates for 24 h at 37° C. Several colonies were streaked onto LB-kan plates and incubated overnight at 42° C. to cure the pKD46 plasmid. Colonies were checked to confirm a phenotype of kanR/ampS. Genomic DNA was isolated from several colonies using the PUREGENE® DNA purification system (Gentra Systems, Inc., Minneapolis, Minn.), and checked by PCR to confirm disruption of the katG gene using primers identified as SEQ ID NO: 38 and SEQ ID NO: 39. Several katG-disrupted strains were transformed with the temperature-sensitive plasmid pCP20 (SEQ ID NO: 40), which contains the FLP recombinase, used to excise the kan gene, and selected on LB-amp plates for 24 h at 37° C., Several colonies were streaked onto LB plates and incubated overnight at 42° C. to cure the pCP20 plasmid. Two colonies were checked to confirm a phenotype of kanS/ampS, and called MG1655 KatG1 and MG1655 KatG2.

Example 2 Construction of a katE Catalase Disrupted E. coli Strain

The kanamycin resistance gene (SEQ ID NO: 30) was amplified from the plasmid pKD13 (SEQ ID NO: 31) by PCR (0.5 min at 94° C., 0.5 min at 55° C., 1 min at 70° C., 30 cycles) using primers identified as SEQ ID NO: 41 and SEQ ID NO: 42 to generate the PCR product identified as SEQ ID NO: 43. The katE nucleic acid sequence is provided as SEQ ID NO: 44 and the corresponding amino acid sequence is SEQ ID NO: 45. E. coli MG1655 (ATCC® 47076™) was transformed with the temperature-sensitive plasmid pKD46 (SEQ ID NO: 37), which contains the λ-Red recombinase genes, and selected on LB-amp plates for 24 h at 30° C. MG1655/pKD46 was transformed with 50-500 ng of the PCR product by electroporation (BioRad Gene Pulser, 0.2 cm cuvette, 2.5 kV, 200 W, 25 μF), and selected on LB-kan plates for 24 h at 37° C. Several colonies were streaked onto LB-kan plates and incubated overnight at 42° C. to cure the pKD46 plasmid. Colonies were checked to confirm a phenotype of kanR/ampS. Genomic DNA was isolated from several colonies using the PUREGENE® DNA purification system, and checked by PCR to confirm disruption of the katE gene using primers identified as SEQ ID NO: 46 and SEQ ID NO: 47. Several katE-disrupted strains were transformed with the temperature-sensitive plasmid pCP20 (SEQ ID NO: 40), which contains the FLP recombinase, used to excise the kan gene, and selected on LB-amp plates for 24 h at 37° C. Several colonies were streaked onto LB plates and incubated overnight at 42° C. to cure the pCP20 plasmid. Two colonies were checked to confirm a phenotype of kanS/ampS, and named MG1655 KatE1 and MG1655 KatE2.

Example 3 Construction of a katG Catalase and katE Catalase Disrupted E. coli Strain (KLP18)

The kanamycin resistance gene (SEQ ID NO: 30) was amplified from the plasmid pKD13 (SEQ ID NO: 31) by PCR (0.5 min at 94° C., 0.5 min at 55° C., 1 min at 70° C., 30 cycles) using primers identified as SEQ ID NO: 41 and SEQ ID NO: 42 to generate the PCR product identified as SEQ ID NO: 43. E. coli MG1655 KatG1 was transformed with the temperature-sensitive plasmid pKD46 (SEQ ID NO: 37), which contains the λ-Red recombinase genes, and selected on LB-amp plates for 24 h at 30° C. MG1655 KatG1/pKD46 was transformed with 50-500 ng of the PCR product by electroporation (BioRad Gene Pulser, 0.2 cm cuvette, 2.5 kV, 200 W, 25 μF), and selected on LB-kan plates for 24 h at 37° C. Several colonies were streaked onto LB-kan plates and incubated overnight at 42° C. to cure the pKD46 plasmid. Colonies were checked to confirm a phenotype of kanR/ampS. Genomic DNA was isolated from several colonies using the PUREGENE® DNA purification system, and checked by PCR to confirm disruption of the katE gene using primers identified as SEQ ID NO: 46 and SEQ ID NO: 47. Several katE-disrupted strains (A katE) were transformed with the temperature-sensitive plasmid pCP20 (SEQ ID NO: 40), which contains the FLP recombinase, used to excise the kan gene, and selected on LB-amp plates for 24 h at 37° C. Several colonies were streaked onto LB plates and incubated overnight at 42° C. to cure the pCP20 plasmid. Two colonies were checked to confirm a phenotype of kanS/ampS, and named MG1655 KatG1KatE18.1 and MG1655 KatG1KatE23. MG1655 KatG1KatE18.1 is designated E. coli KLP18.

Example 4 Cloning and Expression of Perhydrolase from Thermotoga neapolitana

The coding region of the gene encoding acetyl xylan esterase from Thermotoga neapolitana as reported in GENBANK® (Accession No. AAB70869) was synthesized using codons optimized for expression in E, coil (DNA 2.0, Menlo Park, Calif.), The coding region of the gene was subsequently amplified by PCR (0.5 min at 94° C., 0.5 min at 55° C., 1 min at 70° C., 30 cycles) using primers identified as SEQ ID NO: 27 and SEQ ID NO: 28. The resulting nucleic acid product (SEQ ID NO: 29) was subcloned into pTrcHis2-TOPO® to generate the plasmid identified as pSW196. The plasmid pSW196 was used to transform E. coli KLP18 to generate the strain KLP18/pSW196, KLP18/pSW196 was grown in LB media at 37° C. with shaking up to OD_(600nm)=0.4-0.5, at which time IPTG was added to a final concentration of 1 mM, and incubation continued for 2-3 h, Cells were harvested by centrifugation and SDS-PAGE was performed to confirm expression of the perhydrolase at 20-40% of total soluble protein.

Example 5 Fermentation of E. coli KLP18 Transformants Expressing Perhydrolase

A fermentor seed culture was prepared by charging a 2-L shake flask with 0.5 L seed medium containing yeast extract (Amberex 695, 5.0 g/L), K₂HPO₄ (10.0 g/L), KH₂PO₄ (7.0 g/L), sodium citrate dihydrate (1.0 g/L), (NH₄)₂SO₄ (40 g/L), MgSO₄ heptahydrate (1.0 g/L) and ferric ammonium citrate (0.10 g/L). The pH of the medium was adjusted to 6.3 and the medium was sterilized in the flask. Post sterilization additions included glucose (50 wt %, 10.0 mL) and 1 mL ampicillin (25 mg/mL) stock solution. The seed medium was inoculated with a 1-mL culture of E. coli KLP18/pSW196 in 20% glycerol, and cultivated at 35° C. and 300 rpm. The seed culture was transferred at ca. 1-2 OD₅₅₀ to a 14 L fermenter (Braun) with 8 L of medium at 35° C. containing KH₂PO₄ (3.50 g/L), FeSO₄ heptahydrate (0.05 g/L), MgSO₄ heptahydrate (2.0 g/L), sodium citrate dihydrate (1.90 g/L), yeast extract (Amberex 695, 5.0 g/L), Biospumex153K antifoam (0.25 mL/L, Cognis Corporation), NaCl (1.0 g/L), CaCl₂ dihydrate (10 g/L), and NIT trace elements solution (10 mL/L). The trace elements solution contained citric acid monohydrate (10 g/L), MnSO₄ hydrate (2 g/L), NaCl (2 g/L), FeSO₄ heptahydrate (0.5 g/L), ZnSO₄ heptahydrate (0.2 g/L), CuSO₄ pentahydrate (0.02 g/L) and NaMoO₄ dihydrate (0.02 g/L). Post sterilization additions included glucose solution (50% w/w, 80.0 g) and ampicillin (25 mg/mL) stock solution (16.00 mL). Glucose solution (50% w/w) was used for fed batch. Glucose feed was initiated when glucose concentration decreased to 0.5 g/L, starting at 0.31 g feed/min and increasing progressively each hour to 0.36, 0.42, 0.49, 0.57, 0.66, 0.77, 0.90, 1.04, 1.21, 1.41 1.63 g/min respectively; the rate remained constant afterwards. Glucose concentration in the medium was monitored and if the concentration exceeded 0.1 g/L the feed rate was decreased or stopped temporarily. Induction was initiated between OD₅₅₀=56 and OD₅₅₀=80 with addition of 16 mL IPTG (0.5 M) for the various strains. The dissolved oxygen (DO) concentration was controlled at 25% of air saturation. The DO was controlled first by impeller agitation rate (400 to 1400 rpm) and later by aeration rate (2 to 10 slpm). The pH was controlled at 6.8. NH₄OH (29% w/w) and H₂SO₄ (20% w/v) were used for pH control. The head pressure was 0.5 bars. The cells were harvested by centrifugation 16 h post IPTG addition.

Example 6 Preparation of Spray-Dried Thermotoga neapolitana Perhydrolase

Thermotoga neapolitana cell paste prepared as described in Example 5 was suspended at a final concentration of 200 g wet cell weight/L in 50 mM sodium phosphate buffer at pH 7.4. The cells in the suspension were lysed in a single pass through an APV1000 homogenizer operated at 12,000 psi (˜82.74 MPa) inlet pressure. The resulting lysate was heat treated at 65° C. for approximately 30 minutes, and the homogenate cooled to room temperature, and the resulting solids removed by centrifugation. The supernatant from the centrifugation was filtered using a 0.1 micron filter, and the resulting filtrate containing perhydrolase was concentrated using a 30K NMWCO filter to a final protein concentration of 34 mg protein/mL. To the protein solution was added maltodextrin to a concentration of ca. 3-fold by weight that of the concentration of protein, and the resulting solution spray-dried using an inlet temperature of 225° C. and a dryer exit temperature of 76° C. The protein concentration in the resulting powder was 20.3 wt %, and the dry solids content was 93.2 wt %.

Example 7 Effect of Added Solvent on Peracetic Acid Production by Thermotoga neapolitana Perhydrolase

A first mixture of 90.0 g of deionized water, 0.350 g of TURPINAL® SL ((1-hydroxy-1-phosphonoethyl)phosphonic acid, 60 wt % in water; Thermphos International, Hague, Netherlands), and 3.20 g of 30 wt % hydrogen peroxide in water was adjusted to pH 7.2 with 50% aqueous sodium hydroxide, and the final weight of the mixture adjusted to 100.0 g with deionized water. A second mixture of 55.76 g triacetin, 4.20 g of sodium bicarbonate, 2.50 g of CAB-O-SIL® M5 fumed silica (Cabot, Boston, Mass.), 0.270 g of spray-dried Thermotoga neapolitana perhydrolase (Example 6), and 37.43 g of one organic solvent selected from the group consisting of tripropylene glycol methyl ether (DOWANOL® PM; Dow Chemical Corporation, Midland, Mich.), dipropylene glycol methyl ether (DOWANOL® DPM), propylene glycol methyl ether (DOWANOL® PM), diethylene glycol butyl ether (DOWANOL® DB), dipropylene glycol (DOWANOL® DPG), triethylene glycol, 1,2-propanediol, N-ethyl-2-pyrroldinone, isopropanol, ethanol, ethyl lactate, and 1,3-propanediol was prepared. A 1.0 g aliquot of the second mixture was removed with rapid stirring (to suspend the undissolved solids) and mixed with 9.00 mL of the first mixture of hydrogen peroxide, and TURPINAL® SL in water (pH 7.2) was added thereto with stirring at 25° C.; the resulting mixture contained 255 mM triacetin, 254 mM hydrogen peroxide and 0.055 mg protein/mL of spray-dried perhydrolase. A control reaction for each solvent was also run to determine the concentration of peracetic acid produced by chemical perhydrolysis of triacetin by hydrogen peroxide in the absence of added protein.

Determination of the concentration of peracetic acid in the reaction mixtures was performed according to the method described by Karst et al., supra. Aliquots (0.040 mL) of the reaction mixture were removed at predetermined times and mixed with 0.960 mL of 5 mM phosphoric acid in water; adjustment of the pH of the diluted sample to less than pH 4 immediately terminated the reaction. The resulting solution was filtered using an ULTRAFREE® MC-filter unit (30,000 Normal Molecular Weight Limit (NMWL), Millipore Corp., Billerica, Mass.; cat #UFC3LKT 00) by centrifugation for 2 min at 12,000 rpm. An aliquot (0.100 mL) of the resulting filtrate was transferred to 1.5-mL screw cap HPLC vial (Agilent Technologies, Palo Alto, Calif.; #5182-0715) containing 0.300 mL of deionized water, then 0.100 mL of 20 mM MTS (methyl-p-tolyl-sulfide) in acetonitrile was added, the vials capped, and the contents briefly mixed prior to a 10 min incubation at ca. 25° C. in the absence of light. To each vial was then added 0.400 mL of acetonitrile and 0.100 mL of a solution of triphenylphosphine (TPP, 40 mM) in acetonitrile, the vials re-capped, and the resulting solution mixed and incubated at ca. 25° C. for 30 min in the absence of light. To each vial was then added 0.100 mL of 10 mM N,N-diethyl-m-toluamide (DEET; HPLC external standard) and the resulting solution analyzed by HPLC as described below.

HPLC Method:

Supelco Discovery C8 column (10-cm×4.0-mm, 5 μm) (cat. #569422-U) w/precolumn Supelco Supelguard Discovery C8 (Sigma-Aldrich; cat #59590-U); 10 microliter injection volume; gradient method with CH₃CN (Sigma-Aldrich; #270717) and deionized water at 1.0 mL/min and ambient temperature:

Time (min:sec) (% CH₃CN) 0:00 40 3:00 40 3:10 100 4:00 100 4:10 40 7:00 (stop) 40

The peracetic acid concentrations produced in 0.5 min, 1 min, 2 min, 5 min and 10 min for the reactions described above are listed in Table 5, below.

TABLE 5 Dependence of peracetic acid (PAA) concentration on solvent addition using triacetin (255 mM), hydrogen peroxide (254 mM) and 0.055 mg/mL of spray-dried Thermotoga neapolitana perhydrolase. Enzyme PAA (ppm) Solvent (μg/mL) 0.5 min 1 min 2 min 5 min 10 min DOWANOL ® PM 0 39 54 52 81 137 DOWANOL ® DPM 0 136 41 106 386 ND DOWANOL ® TPM 0 23 25 111 93 180 DOWANOL ® DB 0 107 102 105 157 218 DOWANOL ® DPG 0 19 40 101 156 207 Triethylene glycol 0 36 53 110 76 307 1,2-propanediol 0 101 96 122 226 347 N-ethyl-2- 0 37 49 60 77 133 pyrroldinone isopropanol 0 70 13 147 150 242 ethanol 0 68 33 150 356 479 ethyl lactate 0 88 91 98 121 137 1,3-propanediol 0 54 48 48 62 107 DOWANOL ® PM 55 355 1327 1632 3156 5378 DOWANOL ® DPM 55 846 972 1587 3209 4494 DOWANOL ® TPM 55 439 539 1303 2710 3740 DOWANOL ® DB 55 475 827 1719 3222 4863 DOWANOL ® DPG 55 583 769 1211 2784 4522 Triethylene glycol 55 325 834 1634 3229 5116 1,2-propanediol 55 507 903 1428 2921 4364 N-ethyl-2- 55 243 837 1470 3033 4839 pyrroldinone isopropanol 55 326 656 1175 2229 2860 ethanol 55 408 584 1109 2235 2858 ethyl lactate 55 180 337 5736 1420 2554 1,3-propanediol 55 163 269 510 1086 1657

To demonstrate the stability of the spray-dried enzyme in a mixture of triacetin and an organic solvent, the mixtures of triacetin, sodium bicarbonate, CAB-O-SIL® M5 (Cabot), spray-dried Thermotoga neapolitana perhydrolase (Example 6), and either tripropylene glycol methyl ether (DOWANOL® TPM) or 1,2-propanediol described above were stored for 24 h at ambient temperature, then a 1.0 g aliquot of each of these mixtures was removed with rapid stirring (to suspend the undissolved solids) and mixed with 9.0 mL of a freshly prepared (as described above) mixture of hydrogen peroxide and TURPINAL® SL in water (pH 7.2) with stirring at 25° C.; the resulting mixture contained 255 mM triacetin, 254 mM hydrogen peroxide and 0.055 mg protein/mL of spray-dried perhydrolase. A control reaction for each solvent was also run to determine the concentration of peracetic acid produced by chemical perhydrolysis of triacetin by hydrogen peroxide in the absence of added protein. Determination of the concentration of peracetic acid in the reaction mixtures was performed according to the method described by Karst et al. (Table 6).

TABLE 6 Stability of perhydrolase in triacetin/solvent suspension, measured in reactions containing triacetin (255 mM) and hydrogen peroxide (254 mM). Enzyme PAA (ppm) Solvent (μg/mL) 0.5 min 1 min 2 min 5 min 10 min DOWANOL ® TPM 0 0 95 58 172 276 1,2-propanediol 0 16 38 35 171 397 DOWANOL ® TPM 55 386 557 1078 2014 2717 1,2-propanediol 55 566 768 1467 3093 4649

Example 8 Comparison of Peracetic Acid Production by Thermotoga Neapolitana Perhydrolase in Presence or Absence of Added Solvent

A first mixture of 40.0 g of deionized water, 0.1575 g of TURPINAL® SL ((1-hydroxy-1-phosphonoethyl)phosphonic acid, 60 wt % in water; Thermphos International), and 1.44 g of 30 wt % hydrogen peroxide in water was adjusted to pH 7.2 with 50% aqueous sodium hydroxide, and the final weight of the mixture adjusted to 46.87 g with deionized water. A second mixture of 2.78 g triacetin, 0.210 g of sodium bicarbonate, 0.125 g of CAB-O-SIL® M5 (Cabot) and 0.0135 g of spray-dried Thermotoga neapolitana perhydrolase (Example 6) was prepared, and the first mixture of hydrogen peroxide and TURPINAL® SL in water (pH 7.2) was added to the second mixture with stirring at 25° C.; the resulting mixture contained 255 mM triacetin, 254 mM hydrogen peroxide and 0.055 mg protein/mL of spray-dried perhydrolase. Determination of the concentration of peracetic add in the reaction mixtures was performed according to the method described by Karst et al., supra. A control reaction was also run to determine the concentration of peracetic acid produced by chemical perhydrolysis of triacetin by hydrogen peroxide in the absence of added perhydrolase.

The reaction described above was repeated, where 1.872 g of either propylene glycol monomethyl ether (DOWANOL® PM) or dipropyleneglycol monomethyl ether (DOWANOL® DPM), was substituted for an equivalent weight of water in the reaction mixture. A first mixture of 40.0 g of deionized water, 0.175 g of TURPINAL® SL, and 1.60 g of 30 wt % hydrogen peroxide in water was adjusted to pH 7.2 with 50% aqueous sodium hydroxide, and the final weight of the mixture adjusted to 50.0 g with deionized water. A second mixture of 2.78 g triacetin, 1.872 g of either propylene glycol monomethyl ether (DOWANOL® PM) or dipropyleneglycol monomethyl ether (DOWANOL® DPM), 0.210 g of sodium bicarbonate, 0.125 g of CAB-O-SIL® M5 (Cabot) and 0.0135 g of spray-dried Thermotoga neapolitana perhydrolase (Example 6) was prepared, and 45.0 g of the first mixture of hydrogen peroxide and TURPINAL® SL in water (pH 7.2) was added to the second mixture with stirring at 25° C.; the resulting mixture (pH 6.5) contained 255 mM triacetin, 254 mM hydrogen peroxide and 0.055 mg protein/mL of spray-dried perhydrolase. A control reaction was also run to determine the concentration of peracetic acid produced by chemical perhydrolysis of triacetin by hydrogen peroxide in the absence of added extract protein. The peracetic acid concentrations produced in 0.5 min, 1 min, 2 min, 5 min and 10 min for the three reactions described above are listed in Table 7, below.

TABLE 7 Dependence of peracetic acid (PAA) concentration on solvent addition using triacetin (255 mM), hydrogen peroxide (254 mM) and 55 μg/mL of spray-dried Thermotoga neapolitana perhydrolase. Enzyme PAA (ppm) Solvent (μg/mL) 0.5 min 1 min 2 min 5 min 10 min none 0 ND ND ND ND ND DOWANOL ® PM 0 89 90 205 318 498 DOWANOL ® DPM 0 104 178 184 373 535 none 55 629 1359 2020 4274 6019 DOWANOL ® PM 55 807 1390 2331 4439 5917 DOWANOL ® DPM 55 787 1373 2566 5122 6528

Example 9 Use of Solvent for In Situ Peracid Generation Using a Two-Compartment Spray-Bottle Compared to Stirred Reactions

A first mixture of 100 g of 0.20 M sodium citrate buffer containing 2000 ppm TURPINAL® SL ((1-hydroxy-1-phosphonoethyl)phosphonic acid, 60 wt % in water; Thermphos International), 280 g of deionized water, and 5.20 g of 30 wt % hydrogen peroxide in water was adjusted to pH 7.2 with 50% aqueous sodium hydroxide, and the final weight of the mixture adjusted to 400 g with deionized water. A second mixture was separately prepared, containing 83.4 g of triacetin, 3.75 g of CAB-O-SIL® M5 (Cabot), 0.750 g of spray-dried Thermotoga neapolitana perhydrolase (Example 6), and 62.1 g of a single

solvent selected from: propylene glycol methyl ether (DOWANOL° PM), tripropylene glycol methyl ether (DOWANOL® TPM), diethylene glycol methyl ether (DOWANOL® DM), propylene glycol n-butyl ether (DOWANOL® PNB), propylene glycol n-propyl ether (DOWANOL® PnP), propylene glycol monomethyl ether acetate (DOWANOL® PMA), dipropylene glycol, ethanol, isopropanol, and 1,2-propanediol. In a first reaction at 25° C., 1.0 g of the first mixture was stirred with 9.0 g of the second mixture for the first 30-60 seconds of the reaction (reaction pH of 6.5-6.0), and samples were withdrawn and analyzed for peracetic acid production; the resulting reaction mixture contained 255 mM triacetin, 103 mM hydrogen peroxide and 100 μg protein/mL of spray-dried perhydrolase. Determination of the concentration of peracetic acid in the reaction mixtures (TABLE 8, below) was performed according to the method described by Karst et al., supra. A control reaction was also run to determine the concentration of peracetic acid produced by chemical perhydrolysis of triacetin by hydrogen peroxide in the absence of added perhydrolase.

The first mixture and second mixture prepared as described above were each separately charged to one of the two compartments of a two-compartment spray bottle (Custom Dual-Liquid Variable-Ratio Sprayer, Model DLS 200, manufactured by Take5 (Rogue River, Oreg.)), where the bottle was set up to spray a mixture of 9 parts by weight of the first mixture with 1 part by weight of the second mixture. The two mixtures were sprayed into a 12.5 cm diameter crystallizing dish, and the resulting reaction mixture (reaction pH of 6.5-6.0) contained 255 mM triacetin, 100 mM hydrogen peroxide and 0.100 mg protein/mL of spray-dried perhydrolase. The sprayed reaction mixture was sampled at predetermined times and analyzed for peracetic acid (TABLE B, below) according to the method described by Karst et al., supra.

TABLE 8 Dependence of peracetic acid (PAA) concentration on solvent addition using triacetin (255 mM), hydrogen peroxide (103 mM) and 0.100 mg/mL of spray- dried Thermotoga neapolitana perhydrolase in stirred batch reactions and in a sprayed two-component mixture. Enzyme PAA (ppm) Solvent (μg/mL) 20 sec 40 sec 60 sec 120 sec 300 sec 600 sec DOWANOL ® PM, 0 101 106 106 82 90 166 stirred reaction DOWANOL ® PM, 100 319 587 622 648 889 976 stirred reaction DOWANOL ® PM, 100 375 454 515 671 873 994 sprayed reaction DOWANOL ® TPM, 0 0 72 19 25 44 69 stirred reaction DOWANOL ® TPM, 100 445 548 726 980 1378 1560 stirred reaction DOWANOL ® TPM, 100 433 575 1385 806 1089 1250 sprayed reaction DOWANOL ® DM, 0 287 261 287 261 255 234 stirred reaction DOWANOL ® DM, 100 667 875 927 1410 1640 1876 stirred reaction DOWANOL ® DM, 100 540 613 866 914 1112 1276 sprayed reaction DOWANOL ® PNB, 0 76 70 40 58 0 11 stirred reaction DOWANOL ® PNB, 100 344 488 654 932 1166 1357 stirred reaction DOWANOL ® PNB, 100 394 514 586 715 963 1141 sprayed reaction DOWANOL ® PnP, 0 173 163 223 215 213 253 stirred reaction DOWANOL ® PnP, 100 611 716 857 1277 1468 1516 stirred reaction DOWANOL ® PnP, 100 371 657 737 928 1090 1195 sprayed reaction DOWANOL ® PMA, 0 0 0 14 0 128 166 stirred reaction DOWANOL ® PMA, 100 335 510 756 1218 2178 3132 stirred reaction DOWANOL ® PMA, 100 541 745 1042 1472 ND 3236 sprayed reaction dipropylene glycol, 0 26 54 73 79 40 38 stirred reaction dipropylene glycol, 100 318 539 708 1423 1241 946 stirred reaction dipropylene glycol, 100 371 414 464 618 756 863 sprayed reaction ethanol, 0 144 184 152 161 167 170 stirred reaction ethanol, 100 398 553 694 919 1227 1311 stirred reaction ethanol, 100 504 677 685 766 968 1125 sprayed reaction isopropanol, 0 149 167 180 207 180 236 stirred reaction isopropanol, 100 564 691 783 1114 1395 1533 stirred reaction isopropanol, 100 621 767 882 1014 1239 1435 sprayed reaction 1,2-propanediol, 0 32 14 19 33 ND 108 stirred reaction 1,2-propanediol, 100 427 665 921 1485 1941 3466 stirred reaction 1,2-propanediol, 100 376 554 704 1376 1873 2517 sprayed reaction cyclohexanone, 0 136 133 153 138 152 114 stirred reaction cyclohexanone, 100 97 153 185 351 329 459 stirred reaction cyclohexanone, 100 128 196 338 368 416 489 sprayed reaction

Example 10 Peroxycarboxylic Acid Production Using Thermotoga maritima Perhydrolase as an Enzyme Catalyst

Cloning and expression of perhydrolase from Thermotoga maritima is accomplished in accordance with the methods described in preceding Examples 1-4. Fermentation of bacterial transformants expressing Thermotoga maritima perhydrolase is performed in accordance with preceding Example 5, and preparation of spray-dried Thermotoga maritima perhydrolase is accomplished using methods described in Example 6. Additional information regarding techniques for cloning, expressing, and preparation of Thermotoga maritima perhydrolase is available in Published U.S. Patent Application No. 2009/0005590; herein incorporated by reference.

A comparison of peracetic acid production by Thermotoga maritima perhydrolase in the presence and absence of added solvent is performed. A first mixture of 40.0 g of deionized water, 0.1575 g of TURPINAL® SL ((1-hydroxy-1-phosphonoethyl)phosphonic acid, 60 wt % in water; Thermphos International), and 1.44 g of 30 wt % hydrogen peroxide in water is adjusted to pH 7.2 with 50% aqueous sodium hydroxide, and the final weight of the mixture adjusted to 46.87 g with deionized water. A second mixture of 2.78 g triacetin, 0.210 g of sodium bicarbonate, 0.125 g of CAB-O-SIL® M5 (Cabot) and 0.0135 g of spray-dried Thermotoga maritima perhydrolase is prepared, and the first mixture of hydrogen peroxide and TURPINAL® SL in water (pH 7.2) is added to the second mixture with stirring at 25° C.; the resulting mixture containing 255 mM triacetin, 254 mM hydrogen peroxide and 0.055 mg protein/mL of spray-dried perhydrolase. Determination of the concentration of peracetic acid in the reaction mixtures is performed according to the method described by Karst et al., supra. A control reaction is also run to determine the concentration of peracetic acid produced by chemical perhydrolysis of triacetin by hydrogen peroxide in the absence of added perhydrolase.

The reaction described above is repeated, where 1.872 g of either propylene glycol monomethyl ether (DOWANOL® PM) or dipropyleneglycol monomethyl ether (DOWANOL® DPM), is substituted for an equivalent weight of water in the reaction mixture. A first mixture of 40.0 g of deionized water, 0.175 g of TURPINAL® SL, and 1.60 g of 30 wt % hydrogen peroxide in water is adjusted to pH 7.2 with 50% aqueous sodium hydroxide, and the final weight of the mixture adjusted to 50.0 g with deionized water. A second mixture of 2.78 g triacetin, 1.872 g of either propylene glycol monomethyl ether (DOWANOL® PM) or dipropyleneglycol monomethyl ether (DOWANOL® DPM), 0.210 g of sodium bicarbonate, 0.125 g of CAB-O-SIL® M5 (Cabot) and 0.0135 g of spray-dried Thermotoga maritima perhydrolase is prepared, and 45.0 g of the first mixture of hydrogen peroxide and TURPINAL® SL in water (pH 7.2) is added to the second mixture with stirring at 25° C.; the resulting mixture (pH 6.5) contained 255 mM triacetin, 254 mM hydrogen peroxide and 0.055 mg protein/mL of spray-dried perhydrolase. A control reaction is also run to determine the concentration of peracetic acid produced by chemical perhydrolysis of triacetin by hydrogen peroxide in the absence of added extract protein. The peracetic acid concentrations produced in 0.5 min, 1 min, 2 min, 5 min and 10 min for the three reactions described above are measured and recorded.

Example 11 Use of Solvent for In Situ Peracid Generation Using Two-Compartment Spray Device Compared to Stirred Reaction and Using Thermotoga maritima Perhydrolase

A first mixture of 100 g of 0.20 M sodium citrate buffer containing 2000 ppm TURPINAL® SL ((1-hydroxy-1-phosphonoethyl)phosphonic acid, 60 wt % in water; Thermphos International), 280 g of deionized water, and 5.20 g of 30 wt % hydrogen peroxide in water is adjusted to pH 7.2 with 50% aqueous sodium hydroxide, and the final weight of the mixture adjusted to 400 g with deionized water. A second mixture is separately prepared, containing 83.4 g of triacetin, 3.75 g of CAB-O-SIL® M5 (Cabot), 0.750 g of spray-dried Thermotoga maritima perhydrolase (Example 10), and 62.1 g of a single solvent selected from propylene glycol methyl ether (DOWANOL® PM), tripropylene glycol methyl ether (DOWANOL® TPM), diethylene glycol methyl ether (DOWANOL®DM), propylene glycol n-butyl ether (DOWANOL® PNB), propylene glycol n-propyl ether (DOWANOL® PnP), propylene glycol monomethyl ether acetate (DOWANOL® PMA), dipropylene glycol, ethanol, isopropanol, and 1,2-propanediol. In a first reaction at 25° C., 1.0 g of the first mixture is stirred with 9.0 g of the second mixture for the first 30-60 seconds of the reaction (reaction pH of 6.5-6.0), and samples are withdrawn and analyzed for peracetic acid production; the resulting reaction mixture containing 255 mM triacetin, 103 mM hydrogen peroxide and 100 μg protein/mL of spray-dried perhydrolase. Determination of the concentration of peracetic acid in the reaction mixtures is performed according to the method described by Karst et al., supra. A control reaction is also run to determine the concentration of peracetic acid produced by chemical perhydrolysis of triacetin by hydrogen peroxide in the absence of added perhydrolase.

The first mixture and second mixture prepared as described above are each separately charged to one of the two compartments of a two-compartment spray bottle (Custom Dual-Liquid Variable-Ratio Sprayer, Model DLS 200, manufactured by Take5 (Rogue River, Oreg.)), where the bottle is set up to spray a mixture of 9 parts by weight of the first mixture with 1 part by weight of the second mixture. The two mixtures are sprayed into a 12.5 cm diameter crystallizing dish, and the resulting reaction mixture (reaction pH of 6.5-6.0) containing 255 mM triacetin, 100 mM hydrogen peroxide and 0.100 mg protein/mL of spray-dried perhydrolase. The sprayed reaction mixture is sampled at predetermined times and analyzed for peracetic acid according to the method described by Karst et al., supra.

Example 12 Exemplary Two-Component System

One example of a two-component in situ peracid disinfectant formulation is described below.

mol/L grams Component A triacetin 0.100 21.82 T. neapolitana perhydrolase/excipient 0.20 sodium bicarbonate 0.050 4.20 Component B H₂O₂ (30 wt %): 0.100 11.33 TURPINAL ® SL (60 wt %, 0.1% final) 1.67 water (deionized) 960.78 Total weight (grams) 1000.00

For the two-component in situ peracid disinfectant formulation described above, Component A comprises ca. 2.6 wt % of the combined weight of Components A and B, and the weight ratio of Component B to Component A is ca. 38:1. In certain applications for a two-component in situ peracid disinfectant formulation, it may be desirable for the ratio of Component B to Component A to be within a range of from 1:1 to 10:1, where from 10 parts to 1 part (by weight) of Component B is mixed with one part (by weight) of Component A to produce a peracid at a concentration efficacious for disinfection. For example, in a first application a two-compartment spray bottle such as a dual-liquid fixed ratio sprayer (Model DLS100, Take5) or a dual-liquid variable ratio sprayer (Model DLS200, Take5) is utilized, where a maximum ratio of Component B to Component A of 10:1 is employed. In a second application, a single bottle containing two separate compartments separated by a breakable seal is employed, where the ratio of the volume of the two separate compartments is 1:1, or 5:1 or 10:1. In each of these applications, the two-component formulation cannot be mixed at the desired ratio of Component A to Component B to provide the desired concentration of reactants and final concentration of products.

Example 13 Perhydrolysis of Propylene Glycol Diacetate or Ethylene Glycol Diacetate Using Bacillus subtilis ATCC® 31954™ Perhydrolase

A homogenate of a transformant expressing wild-type perhydrolase from Bacillus subtilis ATCC® 31954™ (KLP18/pSW194) was prepared from a suspension of cell paste (20 wt % wet cell weight) in 0.05 M potassium phosphate buffer (pH 7.0) containing dithiothreitol (1 mM). The crude homogenate was centrifuged to remove cellular debris, producing a clarified cell extract that was heat-treated at 65° C. for 30 min. The resulting mixture was centrifuged, and the heat-treated supernatant concentrated on a 30K MWCO (molecular weight cutoff) membrane to a concentration of 32 mg/mL total dissolved solids; a SOS-PAGE of the clarified, heat-treated cell extract indicated that the perhydrolase was at least 85-90% pure. To this concentrate was then added 2.06 grams of NaH₂PO₄ and 1.17 grams Na₂HPO₄ per gram of solids was added to this concentrate to produce an approximate 3:1 ratio (wt/wt) of phosphate buffer to heat-treated cell extract protein. This solution was diluted by 30 wt % with deionized water, then spray-dried (180° C. inlet temperature, 70° C. exit temperature) using a Buchi B-290 laboratory spray dryer); the resulting spray-dried powder contained 25.5 wt % protein (Bradford protein assay) and was 94.3 wt % dry solids.

Reactions (10 mL total volume) were run at 23° C. in 50 mM sodium bicarbonate buffer (initial pH 7.2) containing propylene glycol diacetate (PGDA) or ethylene glycol diacetate (EGDA), hydrogen peroxide (100 mM) and 123 μg/mL of a heat-treated extract protein from the spray-dried E. coli KLP18/pSW194 (expressing Bacillus subtilis ATCC® 31954™ wild-type perhydrolase) (prepared as described above). A control reaction for each reaction condition was run to determine the concentration of peracetic acid produced by chemical perhydroiysis of triacetin by hydrogen peroxide in the absence of added heat-treated extract protein. The reactions were sampled at 1, 5, and 30 minutes and the samples analyzed for peracetic acid using the Karst derivatization protocol (Karst at al., supra); aliquots (0.040 mL) of the reaction mixture were removed and mixed with 0.960 mL of 5 mM phosphoric acid in water; adjustment of the pH of the diluted sample to less than pH 4 immediately terminated the reaction. The resulting solution was filtered using an ULTRAFREE® MC-filter unit (30,000 Normal Molecular Weight Limit (NMWL), Millipore cat #UFC3LKT 00) by centrifugation for 2 min at 12,000 rpm. An aliquot (0.100 mL) of the resulting filtrate was transferred to 1,5-mL screw cap HPLC vial (Agilent Technologies, Palo Alto, Calif.; #5182-0715) containing 0.300 mL of deionized water, then 0.100 mL of 20 mM MTS (methyl-p-tolyl-sulfide) in acetonitrile was added, the vials capped, and the contents briefly mixed prior to a 10 min incubation at ca 25° C. in the absence of light. To each vial was then added 0.400 mL of acetonitrile and 0.100 mL of a solution of triphenylphosphine (TPP, 40 mM) in acetonitrile, the vials re-capped, and the resulting solution mixed and incubated at ca. 25° C. for 30 min in the absence of light. To each vial was then added 0.100 mL of 10 mM N,N-diethyl-m-toluamide (DEET; HPLC external standard) and the resulting solution analyzed by HPLC. The peracetic acid concentrations produced in 1 min, 5 min and 30 min are listed in Table 9.

TABLE 9 Peracetic acid (PAA) concentration produced in reactions utilizing propylene glycol diacetate (PGDA) or ethylene glycol diacetate (EGDA) and hydrogen peroxide (100 mM) in sodium bicarbonate buffer (50 mM, initial pH 7.2) at 23° C. using 123 μg/mL of heat-treated extract protein from E. coli KLP18/pSW194 (Bacillus subtilis ATCC ® 31954 ™ perhydrolase). PAA, PAA, PAA, perhydrolase substrate 1 min 5 min 30 min (50 μg/mL) (100 mM) (ppm) (ppm) (ppm) no enzyme (control) PGDA 0 64 241 B. subtilis ATCC ® 31954 PGDA 666 781 815 no enzyme (control) EGDA 0 18 141 B. subtilis ATCC ® 31954 EGDA 747 931 963

Example 14 Perhydrolysis of Propylene Glycol Diacetate or Ethylene Glycol Diacetate Using T. maritima and T. neapolitana Wild-Type and Variant Perhydrolases

Cell extracts of transformants expressing Thermotoga neapolitana wild-type perhydrolase (KLP18/pSW196), Thermotoga neapolitana C277S variant perhydrolase (KLP18/pSW196/C277S), Thermotoga neapolitana C277T variant perhydrolase (KLP18/pSW196/C277T), Thermotoga maritima wild-type perhydrolase (KLP18/pSW228), Thermotoga maritima C277S variant perhydrolase (KLP18/pSW228/C277S), and Thermotoga maritima C277T variant perhydrolase (KLP18/pSW228/C277T) were each prepared by passing a suspension of cell paste (20 wt % wet cell weight) in 0.05 M potassium phosphate buffer (pH 7.0) containing dithiothreitol (1 mM) twice through a French press having a working pressure of 16,000 psi (˜110 MPa). The lysed cells were centrifuged for 30 minutes at 12,000×g, producing a clarified cell extract that was assayed for total soluble protein (Bradford assay). The supernatant was heated at 75° C. for 20 minutes, followed by quenching in an ice bath for 2 minutes. Precipitated protein was removed by centrifugation for 10 minutes at 11,000×g, SDS-PAGE of the resulting heat-treated extract protein supernatant indicated that the CE-7 enzyme comprised approximately 85-90% of the total protein in the preparation. The heat-treated extract protein supernatant was frozen in dry ice and stored at −80° C. until use

A first set of reactions (10 mL total volume) were run at 20° C. in 10 mM sodium bicarbonate buffer (initial pH 8.1) containing propylene glycol diacetate (PGDA) or ethylene glycol diacetate (EGDA) (100 mM), hydrogen peroxide (100 mM) and 25 μg/mL of heat-treated extract protein from one of E. coli KLP18/pSW195 (Thermotoga neapolitana wild-type perhydrolase), E. coli KLP18/pSW196/C277S (Thermotoga neapolitana C277S variant perhydrolase), E. coli KLP18/pSW196/C277T (Thermotoga neapolitana C277T variant perhydrolase), E. coli KLP18/pSW228 (Thermotoga maritima wild-type perhydrolase), E. coli KLP18/pSW228/C277S (Thermotoga maritima C277S variant perhydrolase), and E. coli KLP18/pSW228/C277T (Thermotoga maritima C277T variant perhydrolase) (prepared as described above). A control reaction for each reaction condition was run to determine the concentration of peracetic acid produced by chemical perhydrolysis of triacetin by hydrogen peroxide in the absence of added extract protein. The reactions were sampled at 1, 5, and 30 minutes and the samples analyzed for peracetic acid using the Karst derivatization protocol (Karst et al., supra) and HPLC analytical method (supra). The peracetic acid concentrations produced in 1 min, 5 min and 30 min are listed in Table 10.

TABLE 10 Peracetic acid (PAA) concentration produced utilizing T. maritima and T. neapolitana wild-type and variant perhydrolases in reactions at 20° C. in sodium bicarbonate buffer (10 mM, initial pH 8.1) containing propylene glycol diacetate (PGDA) (100 mM) or ethylene glycol diacetate (EGDA) (100 mM), hydrogen peroxide (100 mM) and 25 μg/mL of heat-treated extract protein. substrate PAA, PAA, PAA, sub- conc. H₂O₂ 1 min 5 min 30 min perhydrolase strate (mM) (mM) (ppm) (ppm) (ppm) no enzyme PGDA 100 100 0 15 165 (control) T. maritima PGDA 100 100 534 1104 1695 WT T. maritima PGDA 100 100 647 1320 1864 C277S T. maritima PGDA 100 100 656 1174 1418 C277T T. neapolitana PGDA 100 100 513 1052 1946 WT T. neapolitana PGDA 100 100 875 1327 1707 C277S T. neapolitana PGDA 100 100 724 1325 1864 C277T no enzyme EGDA 100 100 0 70 229 (control) T. maritima EGDA 100 100 765 1182 1595 WT T. maritima EGDA 100 100 725 1240 1724 C277S T. maritima EGDA 100 100 802 1218 1734 C277T T. neapolitana EGDA 100 100 603 1132 1643 WT T. neapolitana EGDA 100 100 680 1305 1698 C277S T. neapolitana EGDA 100 100 688 1164 1261 C277T

A second set of reactions (10 mL total volume) were run at 20° C. in 10 mM sodium bicarbonate buffer (initial pH 8.1) containing propylene glycol diacetate (PGDA) or ethylene glycol diacetate (EGDA) (2 mM), hydrogen peroxide (10 mM) and 10 μg/mL of heat-treated extract protein from one of E. coli KLP18/pSW196 (Thermotoga neapolitana wild-type perhydrolase), E. coli KLP18/pSW196/C277S (Thermotoga neapolitana C277S variant perhydrolase), E. coli KLP18/pSW196/C277T (Thermotoga neapolitana C277T variant perhydrolase), E. coli KLP18/pSW228 (Thermotoga maritima wild-type perhydrolase), E. coli KLP18/pSW228/C277S (Thermotoga maritima C277S variant perhydrolase), and E. coli KLP18/pSW228/C277T (Thermotoga maritima C277T variant perhydrolase) (prepared as described above). A control reaction for each reaction condition was run to determine the concentration of peracetic acid produced by chemical perhydrolysis of triacetin by hydrogen peroxide in the absence of added extract protein. The reactions were sampled at 5 minutes and the samples analyzed for peracetic acid using the Karst derivatization protocol (Karst et al., supra) and HPLC analytical method (supra). The peracetic acid concentrations produced in 5 min are listed in Table 11.

TABLE 11 Peracetic acid (PAA) concentration produced utilizing T. maritima and T. neapolitana wild-type and variant perhydrolases in reactions at 20° C. in sodium bicarbonate buffer (10 mM, initial pH 8.1) containing propylene glycol diacetate (PGDA) (2 mM) or ethylene glycol diacetate (EGDA) (2 mM), hydrogen peroxide (10 mM) and 10 μg/mL of heat-treated extract protein. substrate PAA, conc. H₂O₂ 5 min perhydrolase substrate (mM) (mM) (ppm) no enzyme (control) PGDA 2 10 3.6 T. maritima WT PGDA 2 10 5.0 T. maritima C277S PGDA 2 10 7.2 T. maritima C277T PGDA 2 10 7.9 T. neapolitana WT PGDA 2 10 5.7 T. neapolitana C277S PGDA 2 10 7.9 T. neapolitana C277T PGDA 2 10 3.9 no enzyme (control) EGDA 2 10 3.3 T. maritima WT EGDA 2 10 9.9 T. maritima C277S EGDA 2 10 13.6 T. maritima C277T EGDA 2 10 22.9 T. neapolitana WT EGDA 2 10 6.6 T. neapolitana C277S EGDA 2 10 18.4 T. neapolitana C277T EGDA 2 10 20.2

Example 15 Expression of Thermotoga neapolitana Acetyl Xylan Esterase Variants in E. coil KLP18

Plasmids comprising acetyl xylan esterase mutations as described in co-owned, co-filed, and copending U.S. patent application Attorney Docket No. CL4392 US NA were prepared from wild type Thermotoga neapolitana perhydrolase (SEQ ID NO: 14) by substituting at amino acid residue position 277 an Ala, Val, Ser, or Thr (SEQ ID NO: 73). The plasmids were used to transform E. coli KLP18 (Example 3). Transformants were plated onto LB-ampicillin (100 mg/mL) plates and incubated overnight at 37° C. Cells were harvested from a plate using 2.5 mL LB media supplemented with 20% (v/v) glycerol, and 1.0 mL aliquots of the resulting cell suspension frozen at −80° C. One mL of the thawed cell suspension was transferred to a 1-L APPLIKON® Bioreactor (Applikon® Biotechnology, Foster City, Calif.) with 0.7 L medium containing KH₂PO₄ (5.0 g/L), FeSO₄ heptahydrate (0.05 g/L), MgSO₄ heptahydrate (1.0 g/L), sodium citrate dihydrate (1.90 g/L), yeast extract (Amberex 695, 5.0 g/L), Biospumex 153K antifoam (0.25 mL/L, Cognis Corporation), NaCl (1.0 WO, CaCl₂ dihydrate (0.1 g/L), and NIT trace elements solution (10 mL/L). The trace elements solution contained citric acid monohydrate (10 g/L), MnSO₄ hydrate (2 g/L), NaCl (2 g/L), FeSO₄ heptahydrate (0.5 g/L), ZnSO₄ heptahydrate (0.2 g/L), CuSO₄ pentahydrate (0.02 g/L) and NaMoO₄ dihydrate (0.02 g/L). Post sterilization additions included glucose solution (50% w/w, 6.5 g) and ampicillin (25 mg/mL) stock solution (2.8 mL). Glucose solution (50% w/w) was also used for fed batch, Glucose feed was initiated 40 min after glucose concentration decreased below 0.5 g/L, starting at 0.03 g feed/min and increasing progressively each hour to 0.04, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.12, and 0.14 g/min respectively; the rate remaining constant afterwards. Glucose concentration in the medium was monitored, and if the concentration exceeded 0.1 g/L the feed rate was decreased or stopped temporarily. Induction was initiated at OD₅₅₀=50 with addition of 0.8 mL IPTG (0.05 M). The dissolved oxygen (DO) concentration was controlled at 25% of air saturation, first by agitation (400-1000 rpm), and following by aeration (0.5-2 slpm). The temperature was controlled at 37° C., and the pH was controlled at 6.8; NH₄OH (29% w/w) and H₂SO₄ (20% w/v) were used for pH control. The cells were harvested by centrifugation (5,000×g for 15 minutes) at 20 h post IPTG addition. A cell culture of E. coli KLP18/pSW196 (Thermotoga neapolitana wild-type perhydrolase) was grown as described in Example 2.

Example 16 Preparation of Cell Lysates Containing Semi-Purified Wild Type T. Neapolitana Acetyl Xylan Esterase or T. Neapolitana Variant Acetyl Xylan Esterases

A cell culture of E. coli KLP18/pSW196 (Thermotoga neapolitana wild-type perhydrolase) was grown as described in Example 5. The resulting cell paste was resuspended (20% w/v) in 50 mM phosphate buffer pH 7.0 supplemented with 1.0 mM DTT. Resuspended cells were passed through a French pressure cell twice to ensure>95% cell lysis, Lysed cells were centrifuged for 30 minutes at 12,000×g, and the supernatant was heated at 75° C. for 20 minutes, followed by quenching in an ice bath for 2 minutes. Precipitated protein was removed by centrifugation for 10 minutes at 11,000×g. SOS-PAGE indicated that the CE-7 enzyme comprised approximately 85-90% of the total protein in the heat-treated extract supernatant.

Cell cultures of E. coli KLP18/pSW196/C2778 (Thermotoga neapolitana C277S variant perhydrolase), E. coli KLP18/pSW196/C2771/(Thermotoga neapolitana C277V variant perhydrolase), E. coli KLP18/pSW196/C277A (Thermotoga neapolitana C277A variant perhydrolase), and E. coli KLP18/pSW196/C277T (Thermotoga neapolitana C277T variant perhydrolase) were each grown as described in Example 15. The resulting cell pastes were resuspended (20% w/v) in 50 mM phosphate buffer pH 7.0 supplemented with 1.0 mM DTT. Resuspended cells were passed through a French pressure cell twice to ensure>95% cell lysis. Lysed cells were centrifuged for 30 minutes at 12,000×g, and the supernatant was heated at 75° C. for 20 minutes, followed by quenching in an ice bath for 2 minutes. Precipitated protein was removed by centrifugation for 10 minutes at 11,000×g. SDS-PAGE indicated that the CE-7 enzyme comprised approximately 85-90% of the total protein in the heat-treated extract supernatant.

Example 17

Specific Activity and Perhydrolysis/Hydrolysis Ratio of T. Neapolitana Acetyl Xylan Wild-Type Esterase and 0277 Esterase Variants

Reactions (40 mL total volume) were run at 25° C. in phosphate buffer (50 mM, pH 7.2) containing triacetin (100 mM), hydrogen peroxide (100 mM) and one of the following acetyl xylan esterase variants: T. neapolitana C277S variant perhydrolase (0.010 mg/mL of heat-treated extract total protein from E. coli KLP18/pSW196/C277S), T. neapolitana C277T variant perhydrolase (0.010 mg/mL of heat-treated extract total protein from E. coli KLP18/pSW196/C277T), T. neapolitana C277A variant perhydrolase (0.0125 mg/mL of heat-treated extract total protein from E. coli KLP18/pSW196/C277A), and T. neapolitana C277V variant perhydrolase (0.0125 mg/mL of heat-treated extract total protein from E. coli KLP18/pSW196/C277V) (prepared as described in Example 16). Reactions were stirred for only the first 30 seconds of reaction to initially mix the reactants and enzyme.

A reaction was also run under identical conditions to that described immediately above using 0.050 mg/ml of heat-treated extract total protein isolated from E. coli KLP18/pSW196 (expressing Thermotoga neapolitana wild-type acetyl xylan esterase (Example 1)), where the heat-treated extract supernatant was prepared according to the procedure of Example 16.

Two samples from each of the reaction mixtures described above were simultaneously withdrawn after the first minute of each reaction, and every two minutes thereafter for fifteen minutes, where one of the two samples was analyzed for peracetic acid, and the second sample was analyzed for total acetic acid produced from both enzymatic hydrolysis of triacetin and from subsequent conversion of peracetic acid in sample to acetic acid by reaction with methyl-p-tolyl sulfide (MTS, see below).

Measurement of the rate of peracetic acid production in the reaction mixture was performed using a modification of the method described by Karst et al., supra. A sample (0.040 mL) of the reaction mixture was removed at a predetermined time and immediately mixed with 0.960 mL of 5 mM phosphoric acid in water to terminate the reaction by adjusting the pH of the diluted sample to less than pH 4. The resulting solution was filtered using an ULTRAFREE® MC-filter unit (30,000 Normal Molecular Weight Limit (NMWL), Millipore Corp., Billerica, Mass.; cat #UFC3LKT 00) by centrifugation for 2 min at 12,000 rpm. An aliquot (0.100 ml) of the resulting filtrate was transferred to a 1.5-mL screw cap HPLC vial (Agilent Technologies, Palo Alto, Calif.; #5182-0715) containing 0.300 mL of deionized water, then 0.100 mL of 20 mM MTS (methyl-p-tolyl sulfide) in acetonitrile was added, the vial capped, and the contents briefly mixed prior to a 10 min incubation at ca. 25° C. in the absence of light. To the vial was then added 0.400 mL of acetonitrile and 0.100 mL of a solution of triphenylphosphine (TPP, 40 mM) in acetonitrile, the vial recapped, and the resulting solution mixed and incubated at ca. 25° C. for 30 min in the absence of light. To the vial was then added 0.100 mL of 10 mM N,N-diethyl-m-toluamide (DEET; HPLC external standard) and the resulting solution analyzed by HPLC for MTSO (methyl-p-tolyl sulfoxide), the stoichiometric oxidation product produced by reaction of MTS with peracetic acid. A control reaction was run in the absence of added extract protein or triacetin to determine the rate of oxidation of MTS in the assay mixture by hydrogen peroxide, for correction of the rate of peracetic acid production for background MTS oxidation. HPLC method: Supelco Discovery C8 column (10-cm×4.0-mm, 5 μm) (catalog #569422-U) with Supelco Supelguard Discovery C8 precolumn (Sigma-Aldrich; catalog #59590-U); 10 microliter injection volume; gradient method with CH₃CN (Sigma-Aldrich; catalog #270717) and deionized water at 1.0 mL/min and ambient temperature (Table 4).

TABLE 12 HPLC Gradient for analysis of peracetic acid. Time (min:sec) (% CH₃CN) 0:00 40 3:00 40 3:10 100 4:00 100 4:10 40 7:00 (stop) 40

For determination of the rate of perhydrolase-catalyzed acetic acid production in the reaction, a sample (0.900 mL) of the reaction mixture was removed at a predetermined time and immediately added to a 1.5 mL-microcentrifuge tube containing 0.040 mL of 0.75 M H₃PO₄, and the resulting solution briefly mixed to terminate the reaction at pH 3.0-4.0. To the tube was then added 0.020 mL of a solution of 10 mg/mL of Aspergillus niger catalase (Sigma-Aldrich; C3515) in 50 mM phosphate buffer pH (7.2), and the resulting solution mixed and allowed to react for 15 minutes at ambient temperature to disproportionate unreacted hydrogen peroxide to water and oxygen. To the tube was then added 0.040 mL of 0.75 M H₃PO₄ and the resulting solution mixed and filtered using an ULTRAFREE® MC-filter unit (30,000 Normal Molecular Weight Limit (NMWL), Millipore Corp., cat # UFC3LKT 00) by centrifugation for 2 min at 12,000 rpm. An aliquot (0.100 mL) of the resulting filtrate was mixed with 0.150 mL of 20 mM MTS (methyl-p-tolyl sulfide) in acetonitrile, and the resulting solution was incubated for 10 min at ca. 25° C. in the absence of light. The concentration of acetic acid in the sample produced by both enzymatic hydrolysis of triacetin and conversion of peracetic acid to acetic acid by reaction with MTS was determined using a gas chromatograph (GC) equipped with a flame ionization detector (FID) and a DB-FFAP column (length, 15 m; ID, 0.530 mm; film thickness, 1.00 μm); a fresh injection port liner was employed for each rate determination (total of eight sample analyses) to avoid build up of phosphoric acid in the injection port liner over time.

The Thermotoga neapolitana acetyl xylan esterase variants had a significantly-higher specific activity for perhydrolysis of triacetin than the wild-type esterase (Table 13). The perhydrolysis/hydrolysis ratios for the T. neapolitana acetyl xylan esterase variants were determined by dividing the rate of FAA production (perhydrolysis rate) by the rate of hydrolysis of triacetin to acetic acid (hydrolysis rate) (calculated from the rate of total acetic acid production in the assay method from both PM and acetic acid, and corrected for the rate of peracetic acid production); the P/H ratio of the T. neapolitana acetyl xylan esterase variants were ca. equal to or greater than the P/H ratio for the T. neapolitana wild-type acetyl xylan esterase (Table 13).

TABLE 13 specific Thermotoga enzyme perhydrolysis hydrolysis activity neapolitana concen. rate rate P/H (U/mg perhydrolase (μg/mL) (mM/min) (mM/min) ratio protein) wild type 50 3.61 1.22 3.0 72 C277S 10 4.40 1.61 2.7 440 C277T 10 4.24 0.81 5.2 424 C277A 12.5 4.14 1.43 2.9 331 C277V 12.5 3.70 0.88 4.2 296

Example 18 Expression of Thermotoga maritima Acetyl Xylan Esterase Variants in E. coli KLP18

Plasmids comprising acetyl xylan esterase mutations as described in co-owned, co-filed, and copending U.S. patent application Attorney Docket No. CL4392 US NA were prepared from wild type Thermotoga martima (SEQ ID NO: 16) by substituting at amino acid residue position 277 an Ala, Val, Ser, or Thr (SEQ ID NO: 74). The plasmids were used to transform E. coli/KLP18 (Example 3). Transformants were grown in LB media at 37° C. with shaking up to OD_(600nm)=0.4-0.5, at which time IPTG was added to a final concentration of 1 mM, and incubation continued for 2-3 h. Cells were harvested by centrifugation and SDS-PAGE was performed to confirm expression of the acetyl xylan esterase at 20-40% of total soluble protein.

Example 19 Preparation of Cell Lysates Containing Semi-Purified T. Maritima Acetyl Xylan Esterase Variants

Cell cultures (prepared as described in Example 16) were grown using a fermentation protocol similar to that described in Example 15 at a 1-L scale (Applikon). Cells were harvested by centrifugation at 5,000×g for 15 minutes then resuspended (20% w/v) in 50 mM phosphate buffer pH 7.0 supplemented with 1.0 mM DTT. Resuspended cells were passed through a French pressure cell twice to ensure>95% cell lysis. Lysed cells were centrifuged for 30 minutes at 12,000×g, and the supernatant was heated at 75° C. for 20 minutes, followed by quenching in an ice bath for 2 minutes. Precipitated protein was removed by centrifugation for 10 minutes at 11,000×g. SDS-PAGE indicated that the CE-7 enzyme comprised approximately 85-90% of the total protein in the preparation.

Example 20 Specific Activity and Perhydrolysis/Hydrolysis Ratio of T. maritima Acetyl Xylan Wild-Type Esterase and C277 Esterase Variants

Reactions (40 mL total volume) were run at 25° C. in phosphate buffer (50 mM, pH 7.2) containing triacetin (100 mM), hydrogen peroxide (100 mM) and one of the following acetyl xylan esterase variants: T. maritima C277S variant perhydrolase (0.010 mg/mL of heat-treated extract total protein from E. coli KLP18/pSW228/C277S), T. maritima C277T variant perhydrolase (0.010 mg/mL of heat-treated extract total protein from E. coli KLP18/pSW228/C277T), T. maritima C277A variant perhydrolase (0.0125 mg/mL of heat-treated extract total protein from E. coli/KLP18/pSW228/C277A), and T. maritima C277V variant perhydrolase (0.0125 mg/mL of heat-treated extract total protein from E. coli/KLP18/pSW228/C277V) (prepared as described in Example 19). Reactions were stirred for only the first 30 seconds of reaction to initially mix the reactants and enzyme.

A reaction was also run under identical conditions to that described immediately above using 0.050 mg/mL of heat-treated extract total protein isolated from E. coli KLP18/pSW228 (expressing Thermotoga maritima wild-type acetyl xylan esterase), where the heat-treated extract supernatant was prepared according to the procedure of Example 19.

Two samples from each of the reaction mixtures described above were simultaneously withdrawn after the first minute of each reaction, and every two minutes thereafter for fifteen minutes, where one of the two samples was analyzed for peracetic acid using a modification of the method described by Karst et al., supra, and the second sample was analyzed for total acetic acid produced from both enzymatic hydrolysis of triacetin and from subsequent conversion of peracetic acid in sample to acetic acid by reaction with methyl-p-tolyl sulfide (MTS).

The Thermotoga maritima acetyl xylan esterase variants had a significantly-higher specific activity for perhydrolysis of triacetin than the wild type esterase (Table 14). The perhydrolysis/hydrolysis ratios for the T. maritima acetyl xylan esterase variants were determined by dividing the rate of PAA production (perhydrolysis rate) by the rate of hydrolysis of triacetin to acetic acid (hydrolysis rate) (calculated from the rate of total acetic acid production in the assay method from both FAA and acetic acid, and corrected for the rate of peracetic acid production); the P/H ratio of the T. maritima acetyl xylan esterase variants were ca. equal to or greater than the P/H ratio for the T. neapolitana wild-type acetyl xylan esterase (Table 14).

TABLE 14 specific Thermotoga enzyme perhydrolysis hydrolysis activity maritima concen. rate rate P/H (U/mg perhydrolase (μg/mL) (mM/min) (mM/min) ratio protein) wild type 50 3.06 0.47 6.5 61 C277S 10 7.77 0.48 16 777 C277T 10 6.93 1.05 6.6 693 C277A 10 4.27 0.088 48 427 C277V 10 4.25 0.062 68 425

Example 21 Peracetic Add Production Using Perhydrolases

Reactions (100 mL total volume) containing triacetin (2 mM), hydrogen peroxide (10 mM) and from 0.1 μg/mL to 2.0 μg/mL heat-treated cell extract protein (prepared as described above, where the heat-treatment was performed at 85° C. for 20 min) were run in 10 mM sodium bicarbonate buffer (initial pH 8.1) at 20° C. Determination of the concentration of peracetic acid in the reaction mixtures was performed according to the method described by Karst at al., supra. The peracetic acid concentrations produced in 1 min, 5 min, 20 min, 40 min and 60 min are listed in Table 15.

TABLE 15 Dependence of peracetic acid (PAA) concentration on perhydrolase concentration in reactions containing triacetin (2 mM) and hydrogen peroxide (10 mM) in sodium bicarbonate buffer (10 mM, initial pH 8.1) at 20° C., using heat-treated extract protein from E. coli KLP18/pSW228 (Thermotoga maritima wild-type perhydrolase) or E. coli KLP18/pSW228/C277S (Thermotoga maritima C277S variant perhydrolase) (duplicate reactions). Thermotoga enzyme PAA, PAA, PAA, maritima triacetin concen. 1 min 5 min 20 min PAA, 40 min PAA, 60 min perhydrolase (mM) (μg/mL) (ppm) (ppm) (ppm) (ppm) (ppm) no enzyme 2 0 0 0 1 1 3 wild type 2 0.2 0 2 7 13 19 wild type 2 0.2 0 1 5 11 15 wild type 2 0.5 0 2 12 19 25 wild type 2 0.5 0 2 12 21 26 wild type 2 1.0 0 5 20 29 31 wild type 2 1.0 0 5 19 30 31 wild type 2 2.0 1 11 24 24 20 wild type 2 2.0 1 11 29 29 21 C277S 2 0.2 0 4 18 18 18 C277S 2 0.2 0 4 18 17 18 C277S 2 0.5 1 12 39 54 64 C277S 2 0.5 1 10 34 52 64 C277S 2 1.0 18 26 59 69 63 C277S 2 1.0 18 25 60 70 64 C277S 2 2.0 9 38 66 60 48 C277S 2 2.0 9 34 69 61 49

Example 22 Peracetic Acid Production Using Perhydrolases

Reactions (100 mL total volume) containing triacetin (20 mM), hydrogen peroxide (10 mM) and from 0.1 μg/mL to 2.0 μg/mL heat-treated cell extract protein (prepared as described above, where the heat-treatment was performed at 85° C. for 20 min) were run in 10 mM sodium bicarbonate buffer (initial pH 8.1) at 20° C. Determination of the concentration of peracetic acid in the reaction mixtures was performed according to the method described by Karst et al., supra. The peracetic acid concentrations produced in 1 min, 5 min, 20 min, 40 min and 60 min are listed in Table 16.

TABLE 16 Dependence of peracetic acid (PAA) concentration on perhydrolase concentration in reactions containing triacetin (20 mM) and hydrogen peroxide (10 mM) in sodium bicarbonate buffer (10 mM, initial pH 8.1) at 20° C., using heat-treated extract protein from E. coli KLP18/pSW228 (Thermotoga maritima wild-type perhydrolase) or E. coli KLP18/pSW228/C277S (Thermotoga maritima C277S variant perhydrolase) (duplicate reactions). Thermotoga enzyme PAA, PAA, PAA, maritima triacetin concen. 1 min 5 min 20 min PAA, 40 min PAA, 60 min perhydrolase (mM) (μg/mL) (ppm) (ppm) (ppm) (ppm) (ppm) no enzyme 20 0 2 3 3 7 9 wild-type 20 0.2 3 10 15 27 35 wild-type 20 0.2 4 9 19 32 41 wild-type 20 0.5 3 9 21 39 52 wild-type 20 0.5 3 8 22 39 54 wild-type 20 1.0 4 13 35 62 82 wild-type 20 1.0 4 12 37 67 wild-type 20 2.0 9 20 52 91 122 wild-type 20 2.0 10 20 52 87 114 C277S 20 0.2 7 16 67 109 148 C277S 20 0.2 9 24 67 112 144 C277S 20 0.5 16 43 140 202 260 C277S 20 0.5 17 48 148 228 272 C277S 20 1.0 24 75 230 289 353 C277S 20 1.0 26 97 232 297 372 C277S 20 2.0 32 130 318 402 443 C277S 20 2.0 37 135 323 401 430

Example 23 Peracetic Acid Production Using Perhydrolases

Reactions (40 mL total volume) were run at 25° C. in phosphate buffer (50 mM, pH 7.2) containing triacetin (100 mM), hydrogen peroxide (100 mM) and from 10 μg/mL to 50 μg/mL of heat-treated T. neapolitana or T. maritima wild-type or C277 variant perhydrolases (as heat-treated cell extract protein prepared as described above, where the heat-treatment was performed at 75° C. for 20 min). Reactions were stirred for only the first 30 seconds of reaction to initially mix the reactants and enzyme. Determination of the concentration of peracetic acid in the reaction mixtures was performed according to the method described by Karst et al., supra. The peracetic acid concentrations produced in 1 min, 5 min, and 30 min are listed in Table 17.

TABLE 17 Peracetic acid (PAA) production in reactions containing triacetin 100 mM) and hydrogen peroxide (100 mM) in phosphate buffer (50 mM, pH 7.2) at 25° C., using heat-treated T. neapolitana or T. maritima wild-type or C277 variant perhydrolases. enzyme PAA, PAA, PAA, triacetin H₂O₂ concen. 1 min 5 min 30 min perhydrolase (mM) (mM) (μg/mL) (ppm) (ppm) (ppm) no enzyme 100 100 0 63 54 80 T. maritima 100 100 50 529 1790 3785 wild-type T. maritima 100 100 10 979 3241 4635 C277S T. maritima 100 100 10 933 2882 3527 C277T T. maritima 100 100 10 442 2018 2485 C277A T. maritima 100 100 10 577 1931 2278 C277V T. neapolitana 100 100 50 514 1837 3850 wild-type T. neapolitana 100 100 10 606 2237 4609 C277S T. neapolitana 100 100 10 634 2198 3918 C277T T. neapolitana 100 100 12.5 516 2041 3735 C277A T. neapolitana 100 100 12.5 451 1813 2758 C277V

Example 24 Peracetic Add Production Using Perhydrolases

Reactions (10 mL total volume) were run at 25° C. in sodium bicarbonate buffer (1 mM, initial pH 6.0) containing triacetin (100 mM or 150 mM), hydrogen peroxide (100 mM, 250 mM or 420 mM) and heat-treated T. neapolitana or T. maritima wild-type, C277S or C277T variant perhydrolases. Reactions run using 420 mM hydrogen peroxide additionally contained 500 ppm TRUPINAL® SL. Reactions were stirred for only the first 30 seconds of reaction to initially mix the reactants and enzyme. Determination of the concentration of peracetic acid in the reaction mixtures was performed according to the method described by Karst et al., supra. The peracetic acid concentrations produced in 1 min, 5 min, and 30 min are listed in Table 18.

TABLE 18 Peracetic acid (PAA) production in reactions containing triacetin and hydrogen peroxide in bicarbonate buffer (1 mM at pH 6.0 or 100 mM at pH 8.1) or in deionized water (pH 5.0) at 25° C. using heat-treated T. maritima wild- type, C277S or C277T variant perhydrolases. Thermotoga NaHCO₃ PAA, PAA, PAA, maritima triacetin H₂O₂ buffer enzyme concen. 1 min 5 min 30 min perhydrolase (mM) (mM) (mM) (μg/mL) (ppm) (ppm) (ppm) no enzyme 100 100 1.0 0 28 78 141 wild-type 100 100 1.0 75 434 494 608 wild-type 100 100 1.0 100 449 667 643 C277S 100 100 1.0 15 989 1554 1476 C277S 100 100 1.0 20 1301 2139 2131 C277T 100 100 1.0 15 1062 1513 1393 C277T 100 100 1.0 20 996 1430 1516 no enzyme 100 250 0 0 13 71 71 wild-type 100 250 0 75 512 535 533 wild-type 100 250 0 100 576 668 654 C277S 100 250 0 15 653 671 675 C277S 100 250 0 20 943 927 903 C277T 100 250 0 15 717 711 765 C277T 100 250 0 20 730 755 743 no enzyme 150 420 100 0 417 810 848 wild-type 150 420 100 500 6303 8627 9237 C277S 150 420 100 100 7822 10349 10197 

1-22. (canceled)
 23. A multi-component system for producing a peroxycarboxylic acid comprising (a) providing a first component comprising: (i) a carboxylic acid ester substrate; (ii) an enzyme catalyst having perhydrolysis activity, wherein said enzyme catalyst comprises an enzyme having a CE-7 signature motif that aligns with SEQ ID NO: 2 using CLUSTALW, said signature motif comprising: (1) an RGQ motif at amino acid positions aligning with 118-120 of SEQ ID NO:2; (2) a GXSQG motif at amino acid positions aligning with 179-183 of SEQ ID NO:2; and (3) an HE motif at amino acid positions aligning with 298-299 of SEQ ID NO:2; said enzyme comprising at least 90% amino acid identity to SEQ ID NO: 2, 14, 16, 73 or 74; and (iii) at least one cosolvent comprising an organic solvent having a log P of less than about 2, wherein log P is defined as the logarithm of the partition coefficient of a substance between octanol and water, expressed as P=[solute]_(octanol)/[solute]_(water) and wherein the cosolvent is not a substrate for said enzyme catalyst; wherein said first component is a substantially non-aqueous formulation of (i)-(iii); and (b) providing a second component comprising a source of peroxygen in water; wherein said first component and said second component are combined to produce an aqueous reaction formulation comprising a peroxycarboxylic acid and wherein said cosolvent solubilizes the carboxylic acid ester substrate in the aqueous reaction formulation without substantial loss of perhydrolytic activity of the enzyme catalyst.
 24. The system according to claim 23 further comprising a first compartment for storing said first component and a second compartment for storing said second component.
 25. The system according to claim 24 further comprising a mixing compartment for receiving at least some of said first component from said first compartment and at least some of said second component from said second compartment, thereby permitting the formation of a mixture comprising at least some of said first component and at least some of said second component.
 26. The system according to claim 25 wherein the at least some of said first component is mixed with the at least some of said second component in a ratio of about 1:1 to about 1:10 by weight.
 27. The system according to claim 26 further comprising a nozzle for dispensing the aqueous reaction formulation from said mixing compartment.
 28. The system according to claim 27 further comprising a nozzle for receiving at least some of said first component from said first compartment and at least some of said second component from said second compartment, and for dispensing at least some of said first component contemporaneously with at least some of said second component.
 29. The system according to claim 23 wherein the first component and the second component are delivered to a surface with a nozzle permitting the mixing of the first component and the second component on the surface.
 30. The method according to claim 23 wherein said first component is combined with said second component at a ratio of about 1:1 to about 1:10 by weight.
 31. The method according to claim 23 wherein said cosolvent is present in said first component in an amount of about 20% to about 80% by weight.
 32. The method according to claim 23 wherein said substrate is present in said first component in an amount of about 10% to about 60% by weight of that component.
 33. The method according to claim 32 wherein said carboxylic acid ester substrate comprises triacetin, said source of peroxygen comprises hydrogen peroxide, and said enzyme catalyst comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 16 and SEQ ID NO:
 74. 34. The method according to claim 23 wherein said first component comprises about 55% by weight carboxylic acid ester substrate, about 40% by weight cosolvent, about 0.3% by weight enzyme catalyst, and about 2.5% by weight filler, and wherein said second component comprises about 95% by weight water, about 1.5% by weight sodium bicarbonate, and about 1% by weight of a source of peroxygen.
 35. The method according to claim 34 wherein said first component comprises about 55.5% by weight triacetin, about 41% by weight tripropylene glycol methyl ether, about 0.3% by weight of a spray dried enzyme powder comprising Thermotoga neapolitana or Thermotoga maritima perhydrolase or a variant derivative of Thermotoga neapolitana or Thermotoga maritima perhydrolase having one or more point mutations that improve the perhydrolysis activity, and about 2.5% by weight fumed silica, and wherein said second component comprises about 96% by weight water, about 0.2% by weight hydrogen peroxide stabilizer, and about 3.2% by weight of a solution comprising 30% hydrogen peroxide.
 36. The method according to claim 23 wherein, upon combining said first component and said second component to form an aqueous reaction formulation, the initial concentration of said carboxylic acid ester substrate is about 100 mM, the initial concentration of said source of peroxygen is about 100 mM, and the initial concentration of said enzyme catalyst is about 0.1 mg/mL.
 37. The method according to claim 23 wherein, upon combining said first component and said second component to form an aqueous reaction formulation, the initial concentration of said carboxylic acid ester substrate is about 10 mM, the initial concentration of said source of peroxygen is about 10 mM, and the initial concentration of said enzyme catalyst is about 0.01 mg/mL.
 38. The method according to claim 23 wherein said carboxylic acid ester substrate has solubility in water of less than about 100 mg/mL.
 39. The method according to claim 23 wherein said enzyme further comprises an LXD motif at amino acid positions aligning with 179-183 of SEQ ID NO:2.
 40. The method according to claim 23 wherein said enzyme catalyst comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 14 and SEQ ID NO:
 73. 41. The method according to claim 23 wherein said cosolvent comprises tripropylene glycol methyl ether, dipropylene glycol methyl ether, propylene glycol methyl ether, diethylene glycol butyl ether, dipropylene glycol, triethyleneglycol, 1,2-propanediol, N-ethyl-2-pyrroldinone, isopropanol, ethanol, ethyl lactate, 1,3-propanediol, or any combination thereof.
 42. The method according to claim 41 wherein said cosolvent comprises tripropylene glycol methyl ether.
 43. The method according to claim 23 wherein said peroxycarboxylic acid is a C₃-C₁₀ peroxycarboxylic acid.
 44. The method according to claim 23 wherein said peroxycarboxylic acid comprises peracetic acid, perpropionic acid, perbutyric acid, perlactic acid, perglycolic acid, permethoxyacetic acid, per-β-hydroxybutyric acid, or any combination thereof.
 45. The method according to claim 23 wherein enzyme catalyst is provided in the form of a microbial cell, a permeabilized microbial cell, a microbial cell extract, a partially purified enzyme, or a purified enzyme.
 46. The method of claim 45 wherein the enzyme catalyst is immobilized in or on a soluble or an insoluble support.
 47. The method according to claim 23 wherein the enzyme catalyst lacks catalase activity. 